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CN-121975661-A - Multi-source composite enzyme and preparation method and application thereof

CN121975661ACN 121975661 ACN121975661 ACN 121975661ACN-121975661-A

Abstract

The invention provides a multi-source composite ferment, a preparation method and application thereof, and relates to the technical field of biological fermentation. The preparation method of the multi-source composite ferment comprises the steps of ferment booster preparation, primary anaerobic fermentation, secondary anaerobic fermentation and mixing. According to the invention, the ferment booster is prepared by molasses activation culture of the composite microbial agent, and the primary and secondary anaerobic fermentation of plant source, cereal source and animal source raw materials are respectively carried out, and then the ferment booster is compounded in proportion, so that the efficient synergistic fermentation and quality stability control of the multi-source raw materials are realized, and the ferment booster has a good application prospect.

Inventors

  • LIU ZHENG
  • LI SHAOFENG
  • YANG SULI
  • LIU YIZHUO

Assignees

  • 西安煜亿农业科技服务有限责任公司

Dates

Publication Date
20260505
Application Date
20260209

Claims (10)

  1. 1. The preparation method of the multi-source composite ferment is characterized by comprising the following steps of: S1, mixing a composite microbial agent with a molasses matrix in proportion, and performing activation culture to obtain an enzyme booster; S2, mixing molasses, raw materials from different sources and water according to a proportion, adding enzyme booster of S1, and performing anaerobic fermentation in a sealed container; S3, after primary fermentation enters a curing period, adding molasses or brown sugar into different fermentation liquids respectively, and performing secondary anaerobic fermentation to obtain plant source ferment, cereal source ferment and animal source ferment; S4, mixing the plant-derived ferment, the cereal-derived ferment and the animal-derived ferment prepared in the step S3 in proportion to obtain a multi-source composite ferment; In the step S1, the compound microorganisms are saccharomycetes, lactobacillus and acetic acid bacteria, the ratio of the number of the active bacteria of the saccharomycetes, the lactobacillus and the acetic acid bacteria is (2-4): (1-3): (1-2), the temperature of the activation culture is 25-35 ℃, and the time of the activation culture is 24-72 h; In the step S2, the dosage of the ferment booster is 10-30% of the total amount of the mixture, the mass ratio of the molasses to the raw materials to the water is 1 (1.5-4) (5-12), the fermentation temperature of the primary anaerobic fermentation is 20-40 ℃, the fermentation time is 20-40 days, and the stirring is carried out every 5-10 days; In the step S3, the addition amount of molasses or brown sugar is 2% -6% of the fermentation liquor, the fermentation temperature of the secondary anaerobic fermentation is 25-35 ℃, and the fermentation time is 15-30 days; In the step S4, the mass ratio of the plant-derived ferment to the cereal-derived ferment to the animal-derived ferment is (3-5): 2-3): 1-2.
  2. 2. The method for preparing a multi-source composite ferment according to claim 1, wherein in the step S1, the total viable count in the composite microbial agent is 1×10 8 -1×10 10 CFU/g.
  3. 3. The method for preparing the multi-source composite enzyme according to claim 1, wherein in the step S2, the raw materials are plant raw materials, cereal raw materials and animal raw materials, wherein the plant raw materials are selected from one or more of melons, fruits and vegetables, the cereal raw materials are selected from one or more of wheat, corn, soybean and rice, and the animal raw materials are selected from one or more of fish, poultry, meat of livestock or viscera.
  4. 4. The preparation method of the multi-source composite ferment according to claim 1, wherein in the step S2, the anaerobic fermentation is sequentially subjected to a alcoholization period, a acetification period and a ripening period, wherein the pH value of the alcoholization period is 4.0-5.5, the duration is 5-10 days, the pH value of the acetification period is 3.5-4.5, the duration is 5-15 days, the pH value of the ripening period is 3.0-4.0, and the duration is 10-20 days.
  5. 5. The method for preparing the multi-source composite enzyme according to claim 1, wherein in the step S2, the lactobacillus content in the animal-derived enzyme is not less than 3.0X10 6 CFU/g, and the animal-derived enzyme has no fishy smell.
  6. 6. The preparation method of the multi-source composite enzyme according to claim 1, wherein in the step S2, when the raw materials are plant raw materials, the mass ratio of molasses to plant raw materials to water is 1 (2-4): (8-12), the enzyme booster is 10% -20% of the total amount of the mixture, when the raw materials are cereal raw materials, the mass ratio of molasses to cereal raw materials to water is 1 (1.5-2.5): (8-12), the enzyme booster is 10% -20% of the total amount of the mixture, and when the raw materials are animal raw materials, the mass ratio of molasses to animal raw materials to water is 1 (2-4): (5-7), and the enzyme booster is 20% -30% of the total amount of the mixture.
  7. 7. The method for preparing the multi-source composite enzyme according to claim 1, wherein the molasses is cane molasses, and the sugar degree is 40-55 DEG Brix.
  8. 8. The method for preparing the multi-source composite enzyme according to claim 1, further comprising the step S5 of filtering the multi-source composite enzyme obtained in the step S4 to obtain the clarified multi-source composite enzyme.
  9. 9. The multi-source composite enzyme prepared by the method of any one of claims 1 to 8, wherein the pH is 3.0 to 4.5, the total acidity is 15 to 40 g/L, the soluble solids content is 8 to 20 ° Brix, and the total amino acid content is 0.1 to 0.5 g/100 g.
  10. 10. The use of the multi-source composite ferment prepared by the method of any one of claims 1 to 8 in soil remediation, crop pest control and improvement of agricultural product quality.

Description

Multi-source composite enzyme and preparation method and application thereof Technical Field The invention relates to the technical field of biological fermentation, in particular to a multi-source composite ferment, a preparation method and application thereof. Background The ferment is a fermentation product obtained by anaerobic fermentation under the action of microbial metabolism by taking molasses, fruits and vegetables, grains or animal source raw materials and the like as matrixes, contains organic acid, amino acid, vitamins, enzymes and various microbial metabolites, and can be used as an agricultural biological agent in the fields of soil improvement, crop disease and pest control, agricultural product quality improvement and the like. In the prior art, the ferment products are prepared by natural fermentation or single fungus fermentation, and have the defects of slow fermentation, large influence of environmental temperature and raw material difference, easy occurrence of mixed fungus pollution, long fermentation period, poor batch-to-batch stability, no strain activation culture after inoculation, insufficient adaptability and reproductive capacity of the strain, difficult formation of a stable acidification system, insufficient effective fungus content and metabolite accumulation, single source raw materials (such as fruits and vegetables) of the conventional ferment, single carbon-nitrogen ratio and nutrition composition, incomplete spectrum of the metabolite, difficult consideration of the multiple scene requirements of soil restoration, crop health management and the like, easy occurrence of fishy smell, peculiar smell and turbidity precipitation, influence on the sense and application of the product due to higher protein and fat content when the strain system and the process are improperly controlled, easy occurrence of acidity drift, attenuation or layered precipitation of the living fungus, and easy occurrence of spray blockage when suspended matters in liquid are more, and unfavorable popularization in scale. Therefore, it is needed to provide a multi-source composite ferment which can be stably prepared in a short period, has no fishy smell, has balanced components and is stable in storage, and a preparation method thereof, so as to meet application requirements of soil restoration, pest control, improvement of quality of agricultural products and the like in agricultural production. Disclosure of Invention In view of the above, the invention provides a multi-source composite ferment, a preparation method and application thereof. According to the invention, the ferment booster is prepared by molasses activation culture of the composite microbial agent, and the primary and secondary anaerobic fermentation of plant source, cereal source and animal source raw materials are respectively carried out, and then the ferment booster is compounded in proportion, so that the efficient synergistic fermentation and quality stability control of the multi-source raw materials are realized, and the ferment booster has a good application prospect. The first aspect of the invention provides a preparation method of a multi-source composite enzyme, which comprises the following steps: S1, mixing a composite microbial agent with a molasses matrix in proportion, and performing activation culture to obtain an enzyme booster; S2, mixing molasses, raw materials from different sources and water according to a proportion, adding enzyme booster of S1, and performing anaerobic fermentation in a sealed container; S3, after primary fermentation enters a curing period, adding molasses or brown sugar into different fermentation liquids respectively, and performing secondary anaerobic fermentation to obtain plant source ferment, cereal source ferment and animal source ferment; And S4, mixing the plant-derived ferment, the cereal-derived ferment and the animal-derived ferment prepared in the step S3 in proportion to obtain the multi-source composite ferment. Preferably, in the step S1, the compound microorganism is saccharomycetes (Saccharomyces cerevisiae), lactobacillus (Lactobacillus plantarum) and acetic acid bacteria (Acetobacter pasteurianus), the ratio of the number of the living bacteria of the saccharomycetes (Saccharomyces cerevisiae), the lactobacillus (Lactobacillus plantarum) and the acetic acid bacteria (Acetobacter pasteurianus) is (2-4) (1-3) (1-2), the total number of the living bacteria in the compound microorganism microbial inoculum is 1X 10 8-1×1010 CFU/g, the temperature of the activation culture is 25-35 ℃, and the time of the activation culture is 24-72 h. Preferably, in the step S2, the raw materials are plant raw materials, cereal raw materials and animal raw materials, the plant raw materials are one or more of melons, fruits and vegetables, the cereal raw materials are one or more of wheat, corn, soybeans and rice, the animal raw materials are one or more of fish, poultry and livestock bodies