CN-121975666-A - Biocontrol strain BY10 for preventing and treating oat leaf spot disease and application thereof

Abstract

The invention discloses a biocontrol strain BY10 for preventing and treating oat leaf spot disease and application thereof, and belongs to the technical field of functional microorganism screening and application. The biocontrol strain BY10 provided BY the invention belongs to bacillus belicus (Bacillus velezensis), the preservation number is CGMCC No.36208, and the biocontrol strain BY10 has remarkable antagonism on the umbilical vermicularia avenae (DRECHSLERA AVENAE) in oat. The results of the plate counter culture test and the in vitro leaf blade test show that BY10 strain can effectively inhibit the growth and expansion of pathogenic bacteria. The strain has the capabilities of producing protease, producing cellulase and forming a biological film. The invention provides a new strain resource for biological control of oat leaf spot, has the advantages of environmental friendliness, strong targeting property, sustainability and the like, and can be widely applied to control of oat leaf spot.

Inventors

• YAO BO
• CHEN JISHAN
• GAO LIFANG
• ZHU RUIFEN
• LIU CHANG

Assignees

• 重庆市畜牧科学院

Dates

Publication Date

20260505

Application Date

20251221

Claims (7)

1. 1. Bacillus belicus (Bacillus velezensis) is characterized in that the bacillus belicus is a strain BY10, and the preservation number is CGMCC No.36208.
2. 2. Bacillus belgium according to claim 1, wherein strain BY10 is antagonistic to anabrosis avenae (DRECHSLERA AVENAE) and has protease-, cellulase-and biofilm-producing capabilities.
3. 3. Bacillus beleimeris according to claim 1, characterized in that the strain BY10 is isolated from the leaf of oat.
4. 4. The use of bacillus beljalis according to claim 1 for controlling plant diseases, wherein said plant diseases are oat leaf spot.
5. 5. The use according to claim 3, characterized in that it comprises the preparation of a bacterial suspension of said bacillus beijerinus BY10, pre-inoculated on the foliage of oat plants.
6. 6. Use according to claim 3, characterized in that the pathogenic bacteria of the oat leaf spot disease is helminth a umbilical vermicularia (DRECHSLERA AVENAE) in oat.
7. 7. A microbial agent characterized in that the active ingredient is the Bacillus bailii according to claim 1.

Description

Biocontrol strain BY10 for preventing and treating oat leaf spot disease and application thereof Technical Field The invention belongs to the technical field of functional microorganism screening and application, and particularly relates to a biocontrol strain BY10 for preventing and treating oat leaf spot and application thereof. Background Oat (AVENA SATIVA) is a main grain and feed crop in China, and the cultivation area of the feed oat is increased year by year along with the great promotion of policies such as grass husbandry, grain feed change and the like in China. Disease is a major limiting factor in oat production, with leaf spot being one of the major diseases affecting forage oats. The pathogenic bacteria causing leaf spot disease of oat is the vermicularia umbilicifolia (DRECHSLERA AVENAE) in oat, which is particularly serious in low temperature and humidity conditions in the planting area of winter idle fields in southwest. Oat leaf spot disease (Oat leaf spot) is a fungal disease which is easy to occur in a spring humid environment, brown or purple necrotic spots appear on leaves during the occurrence of the disease, and premature senility, reduced photosynthetic capacity and shrunken grains can be caused when the disease is serious. The disease presents a high-onset situation in the major oat producing area of the global temperate zone. Pathogenic bacteria of the oat leaf spot disease are mainly inner navel and child spore bacteria (DRECHSLERA AVENAE), the pathogenic bacteria are transmitted through conidium, and the pathogenic bacteria show strong infectivity at high temperature and high humidity, and seriously influence the oat yield. At present, the prevention and control of oat leaf spot disease is mainly based on chemical bactericides and breeding of disease-resistant varieties. However, long-term use of chemical pesticides has led to increased resistance to pathogenic bacteria and risk of environmental pollution and pesticide residues, while disease resistant varieties have long cultivation periods, high costs, and possible loss of resistance due to genetic variation of pathogenic bacteria. Biological control is becoming a research hotspot in the field of plant disease prevention and control due to the advantages of environmental friendliness, targeting and sustainability. In recent years, research shows that a large number of natural functional strains with biocontrol potential exist in plant phyllosphere and rhizosphere microbial communities, and the biocontrol strains adapting to original host plants can be quickly searched through development of in-situ microbial resources, so that pathogenic bacteria can be effectively prevented and controlled, and meanwhile, the growth of the plants can not be influenced. Therefore, there is a need to screen out a high-efficiency biocontrol strain which can adapt to original host plants, so as to overcome the drug resistance problem and the environmental pollution risk caused by chemical control, solve the limitations of long cultivation period, high cost, easy loss of resistance and the like of disease-resistant varieties, and provide effective guarantee for safe production of oat. Disclosure of Invention The invention aims to provide a biocontrol strain BY10 for preventing and treating oat leaf spot disease and application thereof, and the strain has remarkable antagonism on the oat inner navel vermicularia (DRECHSLERA AVENAE) and can be used for biological prevention and treatment of oat leaf spot disease. In order to achieve the aim of the invention, the invention adopts the following technical scheme: the invention provides bacillus beleiensis (Bacillus velezensis), which is a strain BY10 and is preserved in China general microbiological culture Collection center (CGMCC) with the preservation number of CGMCC No.36208 in the period of 2025 and 10 months and 9 days. Further, the strain BY10 has antagonism to the umbilical vermicularia (DRECHSLERA AVENAE) in oat and has the capabilities of producing protease, producing cellulase and forming biological film. Further, the strain BY10 is isolated from the leaf of Avena sativa Linne. The invention also provides application of the bacillus belicus in preventing and treating plant diseases, wherein the plant diseases are oat leaf spot. Further, the application comprises preparing bacillus beijerinus BY10 into a bacterial suspension and inoculating the bacterial suspension on the leaf surface of the oat plant in advance. Further, the pathogenic bacteria of the oat leaf spot disease is the vermicularia umbilicifolia (DRECHSLERA AVENAE) in oat. The invention also provides a microbial agent, and the active ingredient of the microbial agent is bacillus bailii according to claim 1. The invention has the beneficial effects that: The bacillus belicus BY10 obtained BY screening is obtained BY separating from the leaf of oat, has natural adaptability to original host plants, can effectively inhibit the growth