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CN-121975668-A - Bacillus bailii and application thereof in saline-alkali tolerance and growth promotion of plants

CN121975668ACN 121975668 ACN121975668 ACN 121975668ACN-121975668-A

Abstract

The invention relates to the technical field of microbial fermentation and soil improvement thereof, and particularly discloses bacillus beijerinckii (Bacillus velezensis) and application thereof in plant saline-alkali tolerance and growth promotion. The bacillus belgium (Bacillus velezensis) LAJ1322 has the saline-alkali tolerance, and meanwhile, the fermentation supernatant obtained by fermenting and culturing the strain contains the active ingredients of alkenoic acid and rhodophycin, and potting experiments prove that the fermentation supernatant can reduce the salt-alkali number of soil, improve the content of effective nutrients in the soil, increase the soil fertility, obviously promote the growth of vegetable moss in alkaline lands and effectively improve the biomass and survival of plants.

Inventors

  • LI XIN
  • ZHENG WANNING
  • XU PING
  • PENG DI
  • ZHOU CHI
  • ZHOU MENGQIN
  • HE XIULAN

Assignees

  • 湖南省微生物研究所
  • 河北冠微达生物科技有限公司

Dates

Publication Date
20260505
Application Date
20251222

Claims (11)

  1. 1. The bacillus beleiensis (Bacillus velezensis) LAJ1322 is characterized in that the bacillus beleiensis is preserved in China general microbiological culture collection center (CGMCC) No.36869, and is characterized in that the saline-alkali growth tolerance range is 1% -10% of NaCl concentration and pH7.0-11.
  2. 2. A method for preparing a fermentation supernatant of bacillus belgium (Bacillus velezensis) LAJ1322, the preparation of the fermentation supernatant comprising the steps of: (1) Activating strain, namely inoculating Bacillus bailii (Bacillus velezensis) LAJ1322 to LB solid medium, and culturing at 28-35 ℃ for 1-3 days to obtain activated Bacillus bailii (Bacillus velezensis) LAJ1322; (2) Culturing fermentation seed liquid, namely inoculating activated bacillus beijerinus (Bacillus velezensis) LAJ1322 to an LB liquid culture medium, and culturing in a shaking table at 160-200rpm for 15-20 hours at 25-30 ℃ to obtain seed liquid of bacillus beijerinus (Bacillus velezensis) LAJ 1322; (3) The fermentation supernatant is prepared by inoculating the Bacillus bailii (Bacillus velezensis) LAJ1322 seed solution to a fermentation medium, culturing in a shaking table at 180-220rpm at 33-37deg.C for 24-36 hr, and centrifuging and filtering to obtain fermentation supernatant of Bacillus bailii (Bacillus velezensis) LAJ 1322.
  3. 3. The method according to claim 2, wherein the LB solid medium in the step (1) is formulated of 8-12g/L peptone, 4-6g/L yeast extract, 8-12g/L sodium chloride and 14-16g/L agar powder, pH6.3-6.7.
  4. 4. The method according to claim 2, wherein the LB liquid medium in the step (2) is formulated of 8-12g/L peptone, 4-6g/L yeast extract and 9-11g/L sodium chloride, and has a pH of 6.3-6.7.
  5. 5. The method according to claim 2, wherein the fermentation medium in the step (3) is formulated of molasses 20-25g/L, peptone 8-12g/L, yeast extract 8-10g/L, sodium chloride 6-8g/L, ammonium sulfate 3-5g/L, beef extract 4-6g/L, magnesium sulfate 0.5-0.8g/L, potassium dihydrogen phosphate 1-2g/L and ferric sulfate 0.1-0.3g/L, and has a pH of 6-7.
  6. 6. The method according to claim 2, wherein the inoculation amount in step (3) is 1-5% of the mass of the fermentation medium.
  7. 7. The method according to claim 2, wherein the fermentation supernatant of Bacillus belicus (Bacillus velezensis) LAJ1322 in step (3) comprises the active ingredients enolic acid with CAS number 34422-12-3 and rhodopsin with CAS number 487-79-6.
  8. 8. The method according to claim 7, wherein the mass fraction of the enolic acid and the rhodoic acid in the fermentation broth is 1-3%, and the mass ratio of the enolic acid to the rhodoic acid is 1-7:3-12.
  9. 9. Use of bacillus beijerinus (Bacillus velezensis) LAJ1322 fermentation supernatant according to any one of claims 2-8 in plant salt and alkali tolerance growth promotion.
  10. 10. Use of a fermentation supernatant of bacillus beijerinckii (Bacillus velezensis) LAJ1322 according to any one of claims 2 to 8 for the preparation of a plant salt and alkali tolerant growth promoting product.
  11. 11. Use of a fermentation supernatant of bacillus belgium (Bacillus velezensis) LAJ1322 according to any one of claims 2-8 in soil improvement in saline-alkali soil.

Description

Bacillus bailii and application thereof in saline-alkali tolerance and growth promotion of plants Technical Field The invention relates to the technical field of microbial fermentation and soil improvement thereof, in particular to bacillus bailii and application thereof in the aspect of saline-alkali resistance and growth promotion of plants. Background Saline-alkali soil refers to soil with the content of soluble salt substances in the soil being more than 2g/kg and the pH value being more than 8.0. Soil salinization directly affects the normal physiological metabolism and growth and development of plants, and has become one of the main abiotic stress factors limiting crop productivity. The saline-alkali soil area of China is about 9.9X10 7hm2, which accounts for 10% of the land area of China, and large-area saline-alkali soil is an important backup land resource of China. However, the saline-alkali soil has high Na + content, strong alkalinity, poor air permeability and low fertility, and the soil improvement and utilization difficulty is high. How to improve the saline-alkali soil, promote the adaptive growth of saline-alkali soil plants and increase the crop yield in the saline-alkali soil becomes a research hot spot in recent years. Compared with other improvement measures, the improvement of the saline-alkali soil by utilizing the beneficial functional microorganisms has the advantages of ecological environment protection, no pollution, good effect, long duration and the like. The acid substances, plant growth hormone, permeation regulating substances and the like secreted by the functional microorganisms play an important role in relieving stress environment, improving physical and chemical properties of soil and improving soil fertility, and can achieve the effect of improving the yield and quality of planted crops in saline-alkali soil. However, different microorganisms secrete different metabolites, and the growth promotion effect of the microorganisms on saline-alkali soil crops is greatly different, CN121022683A discloses bacillus subtilis HP26 with salt tolerance and growth promotion effect, a microbial agent and application, but bacillus subtilis HP26 can only grow in the range of 0-6% of NaCl concentration, and CN118344995A discloses application of bacillus subtilis LAJ1322RKN1111 in saline-alkali soil treatment and plant growth promotion, but bacillus subtilis RKN1111 cannot survive in a strong alkaline environment. Therefore, screening of functional strains which can adapt to saline-alkali environment and can synthesize the metabolic products related to improving soil conditions and promoting crop growth is one of key measures for improving the utilization rate of saline-alkali soil. Disclosure of Invention The invention aims to provide bacillus beijerinus (Bacillus velezensis) LAJ1322, a functional metabolite and application thereof in plant saline-alkali tolerance growth promotion. In order to achieve the above purpose, the present invention provides the following technical solutions: The invention provides bacillus beleiensis (Bacillus velezensis) LAJ1322 which is preserved in China center for type culture collection, wherein the preservation number is CGMCC No.36869, the preservation address is the No.3 of the West Song No. 1 of the Chaoyang area North Star of Beijing, and the preservation time is 2025 and 12 months 2. The invention also provides a fermentation supernatant, which is prepared from the bacillus beijerinus (Bacillus velezensis) LAJ 1322. The preparation of the fermentation broth comprises the following steps: (1) Activating strain, namely inoculating Bacillus bailii (Bacillus velezensis) LAJ1322 to LB solid medium, and culturing at 28-35 ℃ for 1-3 days to obtain activated Bacillus bailii (Bacillus velezensis) LAJ1322; (2) Culturing fermentation seed liquid, namely inoculating activated bacillus beijerinus (Bacillus velezensis) LAJ1322 to an LB liquid culture medium, and culturing in a shaking table at 160-200rpm for 15-20 hours at 25-30 ℃ to obtain seed liquid of bacillus beijerinus (Bacillus velezensis) LAJ 1322; (3) The fermentation supernatant is prepared by inoculating the Bacillus bailii (Bacillus velezensis) LAJ1322 seed solution to a fermentation medium, culturing in a shaking table at 180-220rpm at 33-37deg.C for 24-36 hr, and centrifuging and filtering to obtain fermentation supernatant of Bacillus bailii (Bacillus velezensis) LAJ 1322. Further, in the step (1), the formula of the LB solid medium is 8-12g/L peptone, 4-6g/L yeast extract, 8-12g/L sodium chloride and 14-16g/L agar powder, and the pH value is 6.3-6.7. Further, in the step (2), the LB liquid medium has a formula of 8-12g/L peptone, 4-6g/L yeast extract and 9-11g/L sodium chloride, and the pH is 6.3-6.7. Further, the formula of the fermentation medium in the step (3) comprises 20-25g/L of molasses, 8-12g/L of peptone, 8-10g/L of yeast extract powder, 6-8g/L of sodium chloride, 3-5g/L of ammonium sulfa