Search

CN-121975669-A - Deep sea source bacillus paralicheniformis BC20 and application thereof in cosmetics

CN121975669ACN 121975669 ACN121975669 ACN 121975669ACN-121975669-A

Abstract

The invention provides deep sea source bacillus paralicheniformis BC20 and application thereof in cosmetics. The invention obtains the side bacillus licheniformis BC20 with broad-spectrum antibacterial activity from deep sea through research, and has the characteristics of salt resistance, heat resistance and the like. The fermentation product and the antibacterial peptide of the bacillus paratyphenius BC20 have broad-spectrum antibacterial activity and have good inhibition effect on various bacteria and fungi. In particular, the bacillus paratyphenius BC20 has good inhibition effect on Burkholderia pollution for the first time. The bacillus thuringiensis BC20, the fermentation product and the antibacterial peptide can be used as a bacteriostatic agent in fields including cosmetics and the like.

Inventors

  • CHAN ZHUHUA
  • DAI SHUZHEN
  • Du Yuanshuai
  • YI ZHIWEI
  • ZENG RUNYING

Assignees

  • 自然资源部第三海洋研究所

Dates

Publication Date
20260505
Application Date
20251222
Priority Date
20251215

Claims (11)

  1. 1. The bacillus licheniformis (Bacillus paralicheniformis) BC20 is characterized in that the preservation number of the BC20 is CCTCC No. M20232179.
  2. 2. Use of the bacillus paralicheniformis (Bacillus paralicheniformis) BC20 of claim 1, the fermentation product of the bacillus paralicheniformis (Bacillus paralicheniformis) BC20 of claim 1 or the antimicrobial peptide of the bacillus paralicheniformis (Bacillus paralicheniformis) BC20 of claim 1 for the preparation of a bacteriostatic agent for inhibiting bacterial and/or fungal growth.
  3. 3. The use according to claim 2, wherein the bacteria comprise Burkholderia contaminated, escherichia coli, staphylococcus aureus, salmonella, pseudomonas aeruginosa, vibrio alginolyticus, vibrio harveyi, vibrio parahaemolyticus, and/or, The fungi include candida albicans, mucor, aspergillus niger and aspergillus oryzae.
  4. 4. Use of the bacillus paralicheniformis (Bacillus paralicheniformis) BC20 of claim 1, the fermentation product of the bacillus paralicheniformis (Bacillus paralicheniformis) BC20 of claim 1 or the antimicrobial peptide of the bacillus paralicheniformis (Bacillus paralicheniformis) BC20 of claim 1 as a bacteriostatic agent in the preparation of cosmetics.
  5. 5. The use according to claim 4, wherein the cosmetic comprises essence, lotion, cream, face cream, spray.
  6. 6. The use according to any one of claims 2 to 5, wherein the preparation method of the fermentation product comprises the steps of (1) inoculating the bacillus licheniformis (Bacillus paralicheniformis) BC20 into a culture medium, performing fermentation culture at 30-37 ℃, and (2) collecting fermentation liquor, centrifuging, and taking a supernatant to obtain the fermentation product.
  7. 7. The application of any one of claims 2-5, wherein the preparation method of the antibacterial peptide comprises the steps of (1) inoculating the bacillus licheniformis (Bacillus paralicheniformis) BC20 into a culture medium, fermenting and culturing at 30-37 ℃, 2) collecting fermentation liquor, centrifuging, taking supernatant, adjusting pH to 2.0-4.0, uniformly stirring to form suspension, standing at 2-6 ℃ for 8-12 h, 3) discarding supernatant, centrifuging, collecting sticky precipitate, 4) extracting the precipitate with a solvent until the precipitate is fully dissolved in the solvent, and merging the extracts to obtain the antibacterial peptide.
  8. 8. The method according to claim 6 or 7, wherein the medium is LB medium.
  9. 9. The use according to claim 8, wherein the medium further comprises at least one of glucose, glucosamine and glutamic acid; Preferably, the concentration of glucose in the culture medium is 1-2 g/L, and/or, The concentration of the glucosamine in the culture medium is 1-2 g/L, and/or, The concentration of the glutamic acid in the culture medium is 0.2-0.4 g/L.
  10. 10. A bacteriostat, characterized in that the active ingredient of the bacteriostat comprises the bacillus paralicheniformis (Bacillus paralicheniformis) BC20 of claim 1, the fermentation product of the bacillus paralicheniformis (Bacillus paralicheniformis) BC20 of claim 1 and/or the antimicrobial peptide of the bacillus paralicheniformis (Bacillus paralicheniformis) BC20 of claim 1.
  11. 11. A cosmetic product, which is characterized in that, the cosmetic comprising the bacteriostatic agent according to claim 10.

Description

Deep sea source bacillus paralicheniformis BC20 and application thereof in cosmetics Technical Field The invention belongs to the technical field of microorganisms, in particular to marine microorganisms, and particularly relates to deep-sea-source bacillus licheniformis BC20 and application thereof in cosmetics. Background With the development of economy and the improvement of the requirements of people on the good living standard, the requirements of people on the quality of the clothes and the food are gradually improved, and the safety requirements of antibacterial and antiseptic substances used in cosmetics, foods, medicines and feed additives are strict. The cosmetics usually use chemicals such as nipagin ester, phenoxyethanol and the like as preservative, the foods usually use potassium sorbate and sodium benzoate as preservative, and the medical and feed additives produce antibiotics as antibacterial or preservative. However, most of the above antibacterial or antiseptic agents are chemical substances, which have certain toxicity, and excessive or improper use can cause allergic reaction of human body, so that the allowable use range of the antiseptic agent and the use concentration thereof are limited. The current bacteriostasis methods mainly comprise a physical method and a chemical method. Physical heating or irradiation may affect product quality, while chemical/pharmaceutical treatment may lead to incomplete sterilization, and the use of antibiotics is generally limited to medical use and is not applicable to foods and cosmetics. Biological antimicrobial methods, particularly those involving antagonism of microorganisms, are of great interest due to their ecological safety and low risk of resistance. The microbial antibacterial peptides are various, but have few broad-spectrum bacteriostasis and single product application. Therefore, the field needs to develop novel microorganisms with good antibacterial effect and metabolites (including antibacterial peptides) thereof, improve antibacterial activity and meet the demands of different fields. Disclosure of Invention Based on this, the invention aims to provide deep sea-derived Bacillus paratyphilis BC20 and application thereof in cosmetics. In order to achieve the above purpose, the present invention adopts the following technical scheme. In a first aspect of the invention, there is provided a Bacillus paratungstate (Bacillus paralicheniformis) BC20, wherein the preservation number of the BC20 is CCTCC No. M20232179. In a second aspect of the invention there is provided the use of an antimicrobial peptide of Bacillus paratlicheniformis (Bacillus paralicheniformis) BC20 as described above, a fermentation product of Bacillus paratlicheniformis (Bacillus paralicheniformis) BC20 as described above or Bacillus paratlicheniformis (Bacillus paralicheniformis) BC20 as described above in the preparation of a bacteriostatic agent for inhibiting bacterial and/or fungal growth. In some embodiments, the bacteria include burkholderia, escherichia coli, staphylococcus aureus, salmonella, pseudomonas aeruginosa, vibrio alginolyticus, vibrio harveyi, vibrio parahaemolyticus. In some embodiments, the fungus comprises candida albicans, mucor, aspergillus niger, aspergillus oryzae. In a third aspect of the invention there is provided the use of a Bacillus paratlicheniformis (Bacillus paralicheniformis) BC20 as described above, a fermentation product of a Bacillus paratlicheniformis (Bacillus paralicheniformis) BC20 as described above or an antimicrobial peptide of a Bacillus paratlicheniformis (Bacillus paralicheniformis) BC20 as described above as a bacteriostatic agent in the preparation of a cosmetic. In some embodiments, the cosmetic comprises a serum, an emulsion, a skin care cream, a face cream, a spray. In some embodiments, the preparation method of the fermentation product comprises the following steps of (1) inoculating the bacillus paratolus (Bacillus paralicheniformis) BC20 into a culture medium, fermenting and culturing at 30-37 ℃, and (2) collecting fermentation liquid, centrifuging, and taking supernatant to obtain the fermentation product. The preparation method of the antibacterial peptide comprises the following steps of (1) inoculating the bacillus licheniformis (Bacillus paralicheniformis) BC20 into a culture medium, fermenting and culturing at the temperature of 30-37 ℃, 2) collecting fermentation liquor, centrifuging, taking supernatant, adjusting the pH to 2.0-4.0, uniformly stirring to form suspension, standing for 8-12 h at the temperature of 2-6 ℃, 3) discarding supernatant, centrifuging, collecting sticky precipitate, 4) extracting the precipitate by using a solvent until the precipitate is fully dissolved in the solvent, and merging the extracting solution to obtain the antibacterial peptide. In some embodiments, the solvent is selected from methanol and/or ethanol. In some embodiments, the medium is LB medium. In some embodiments, the m