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CN-121975671-A - Bacillus desert B-3 with broad-spectrum antibacterial activity and application thereof

CN121975671ACN 121975671 ACN121975671 ACN 121975671ACN-121975671-A

Abstract

The invention discloses a bacillus desert (Bacillus inaquosorum) B-3 with broad-spectrum antibacterial activity and application thereof. The bacillus desert B-3 is preserved in China general microbiological culture Collection center (CGMCC) with a preservation date of 2025, 8 and 28 days and a preservation number of CGMCC NO.35756. The B3 strain is the first reported desert bacillus with the capability of antagonizing the clubroot, has antagonism on various plant pathogenic bacteria including the botrytis cinerea, the fusarium, the sclerotinia sclerotiorum and the sclerotium rolfsii, can be used as biological pesticide, has good effect on the clubroot, obviously increases the fresh weight of plants infected by the clubroot Pb4 after B-3, and obviously increases the number of plant root systems, thus indicating that the biocontrol strain can effectively prevent and treat the clubroot and has antibacterial and growth promoting effects.

Inventors

  • Che Shu
  • HUANG RONG
  • HU JIANKUN

Assignees

  • 江西省农业科学院植物保护研究所

Dates

Publication Date
20260505
Application Date
20251226

Claims (10)

  1. 1. The bacillus desert (Bacillus inaquosorum) B-3 is characterized by being preserved in China general microbiological culture collection center (CGMCC) with a preservation date of 2025, 8 and 28 days and a preservation number of CGMCC NO.35756.
  2. 2. The microbial inoculum of claim 1 prepared from bacillus desert (Bacillus inaquosorum) B-3.
  3. 3. The microbial inoculum according to claim 2, wherein the microbial inoculum is prepared by inoculating the bacillus desert (Bacillus inaquosorum) B-3 into liquid LB for 3-4 days at 28 ℃ with shaking of 220: 220 rpm.
  4. 4. The supernatant produced by bacillus desert (Bacillus inaquosorum) B-3 of claim 1.
  5. 5. The supernatant according to claim 2, wherein the supernatant is prepared by inoculating the bacillus desert B-3 into a fresh sterile liquid LB, shaking culturing for 3-4 days at 28 ℃ and 220 rpm, taking the cultured bacterial liquid, 6000 rpm, centrifuging 10min, taking the centrifuged supernatant, and filtering the bacterial filter for immediate use.
  6. 6. The use of any one of the following bacillus desert (Bacillus inaquosorum) B-3 of claim 1: (1) Inhibiting any one or more of rhizomatosis bacteria, botrytis cinerea, fusarium, sclerotinia sclerotiorum and sclerotium rolfsii; (2) Preventing and treating plant diseases caused by any one of rhizoctonia, botrytis cinerea, fusarium, sclerotinia sclerotiorum and sclerotium rolfsii; (3) Preparing a biological pesticide, wherein the biological pesticide inhibits any one or more pathogenic bacteria in rhizopus, botrytis cinerea, fusarium, sclerotinia sclerotiorum; (4) Promote plant growth.
  7. 7. Use of the microbial agent of claim 2 or 3 for any of the following: (1) Inhibiting any one or more of rhizomatosis bacteria, botrytis cinerea, fusarium, sclerotinia sclerotiorum and sclerotium rolfsii; (2) Preventing and treating plant diseases caused by any one of rhizoctonia, botrytis cinerea, fusarium, sclerotinia sclerotiorum and sclerotium rolfsii; (3) Preparing a biological pesticide, wherein the biological pesticide inhibits any one or more pathogenic bacteria in rhizopus, botrytis cinerea, fusarium, sclerotinia sclerotiorum; (4) Promote plant growth.
  8. 8. Use of any one of the supernatants of claim 4 or 5: (1) Inhibiting any one or more of rhizomatosis bacteria, botrytis cinerea, fusarium, sclerotinia sclerotiorum and sclerotium rolfsii; (2) Preventing and treating plant diseases caused by any one of rhizoctonia, botrytis cinerea, fusarium, sclerotinia sclerotiorum and sclerotium rolfsii; (3) Preparing a biological pesticide, wherein the biological pesticide inhibits any one or more pathogenic bacteria in rhizopus, botrytis cinerea, fusarium, sclerotinia sclerotiorum; (4) Promote plant growth.
  9. 9. A method for controlling clubroot of crucifer crops, comprising applying the bacillus desert of claim 1B-3 or the microbial inoculum of claim 2 to roots or planting soil of crucifer crops.
  10. 10. The control method according to claim 9, wherein the application mode is one or more of root irrigation, soil spraying or soil mixing, preferably root irrigation, each plant is applied with the microbial inoculum or supernatant liquid of 5 ml, and the application time is within 3-7 days after the plant emergence.

Description

Bacillus desert B-3 with broad-spectrum antibacterial activity and application thereof Technical Field The invention belongs to the field of biological agriculture, and relates to bacillus desert B-3 with broad-spectrum antibacterial activity and application thereof. Background Soil-borne plant diseases form a continuous threat to agricultural production, wherein clubroot caused by brassica radiata is particularly prominent, and the management of the clubroot is called as a worldwide difficult problem. The dormant spores of the pathogenic bacteria can survive for more than ten years in the soil, and the transmission paths are various, so that once the field is polluted, the dormant spores are extremely difficult to eradicate. Currently, chemical pesticides have little effect on dormant spores of this bacterium and are difficult to act on pathogens that have invaded plant roots. Although the soil fumigation and other powerful chemical methods have certain effects, the cost is high, the operation is dangerous, the soil micro-ecology is seriously destroyed, and the method runs counter to the sustainable development of agriculture. Faced with this serious challenge, microbial antagonists are considered a promising green control approach. However, the biocontrol strains of the prior art often show significant limitations when dealing with plasmodiophora. Many strains have a narrow bacteriostasis spectrum and cannot simultaneously cope with secondary diseases caused by clubroot. More importantly, the acid or barren soil environment in which clubroot often occurs can severely restrict the survival, colonization and activity of most biocontrol bacteria, so that the field control effect is unstable, and the laboratory effect is difficult to reproduce. Thus, there is a great need in the art for a biocontrol microbial resource that is specifically adapted to and effective against the unique and persistent pathogen, plasmodium rhizomatous. The ideal new strain should be capable of effectively colonising in the severe soil environment where the clubroot bacteria live, and have broad-spectrum antagonistic activity to cope with compound infection, thereby providing a brand new solution for overcoming the chronic clubroot. The desert bacillus is an important member of bacillus, and is gradually deepened in research and application in the field of plant protection due to the characteristics of strong stress resistance, rich metabolites and the like. The disclosed research shows that the strain has proved to have inhibition effect on a plurality of plant pathogenic bacteria such as fusarium pseudocladi, fusarium graminearum, cotton fusarium wilt and the like, can realize disease prevention and control through mechanisms such as synthesis of antibacterial compounds, competition of ecological niches, induction of plant system resistance and the like, and has the multifunctional characteristics of promoting plant growth, degrading agricultural wastes and the like. The culture conditions are standardized step by step, the detection technology is optimized continuously, a foundation is laid for large-scale application, but up to the present, no public report has been made to confirm that the bacillus desert has the function of preventing and treating clubroot, and targeted strain screening and application research is lacking. Disclosure of Invention The invention aims at providing the bacillus desert B-3 with broad-spectrum antibacterial activity and the application thereof, aiming at the defects of the prior art. The invention also aims to provide a microbial inoculum, supernatant fluid and application thereof prepared by the bacillus desert B-3. It is still another object of the present invention to provide a method for controlling clubroot of cruciferous crops. The aim of the invention can be achieved by the following technical scheme: The bacillus desert (Bacillus inaquosorum) B-3 is preserved in China general microbiological culture Collection center (China center for type culture collection) with a preservation date of 2025, 8 and 28 days and a preservation number of CGMCC No.35756. The desert bacillus (Bacillus inaquosorum) B-3 is separated from eggplant stalks, is an opaque yellowish white circular colony on an LB plate, can grow on LB, PDA and YPDA culture mediums, has an optimal growth temperature of 28 ℃, and can produce protease and cellulase. The bacterial agent prepared by the bacillus desert (Bacillus inaquosorum) B-3. Preferably, the microbial inoculum is prepared by inoculating the bacillus desert bacillus (Bacillus inaquosorum) B-3 into fresh sterile liquid LB for 3-4 days at 28 ℃ and shaking culture of 220: 220 rpm. The supernatant prepared by the bacillus desert (Bacillus inaquosorum) B-3. Preferably, the supernatant is prepared by inoculating the bacillus desert (Bacillus inaquosorum) B-3 into fresh sterile liquid LB, shaking culturing at 28 ℃ for 3-4 days at 220-rpm, collecting the shaking cultured bacterial