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CN-121975673-A - Novel amylase and cellulase producing microbacterium strain and application thereof in hermetia illucens cultivation and growth promotion

CN121975673ACN 121975673 ACN121975673 ACN 121975673ACN-121975673-A

Abstract

A novel micro-bacillus (Microbacterium) strain for promoting the growth of black soldier fly larvae and application thereof are provided, wherein the strain comprises a 16S rDNA sequence shown in SEQ ID NO. 1 or a complementary sequence thereof or a sequence with at least 85% nucleic acid identity compared with SEQ ID NO. 1. The Microbacterium sp.Micr. M175 of the present invention is separated from the insect sand and has the characteristic of decomposing starch and cellulose. The addition of the fungus in the hermetia illucens breeding process can promote the growth of larvae and promote the accumulation of protein and fat of the larvae. After micr.m175 addition, the hermetia illucens larvae grew by 2.94% and 21.19% in length and by 32.41% and 47.74% in accumulation of protein and fat, respectively, as compared to the addition of sterile water. Therefore, the high-quality and high-efficiency feeding of the hermetia illucens is realized, and the industrialized production of the hermetia illucens body is facilitated.

Inventors

  • JU FENG
  • WANG YUMEI
  • FENG SONGHUI
  • TIAN JING

Assignees

  • 牧原实验室
  • 西湖大学

Dates

Publication Date
20260505
Application Date
20251226

Claims (9)

  1. 1. A microbacterium comprising the 16S rDNA sequence set forth in SEQ ID No.1 or its complement or a sequence at least 85% identical to SEQ ID No. 1.
  2. 2. The Microbacterium of claim 1, wherein said Microbacterium has a 16S rDNA sequence as shown in SEQ ID NO. 1, which is Microbacterium sp. Micr. M175 with a preservation number of CGMCC No. 35903, which was preserved in China general microbiological culture Collection center (China general microbiological culture Collection center) at 9 and 12 of 2025.
  3. 3. A method for culturing a microbacterium according to claim 1 or 2, characterized in that it comprises inoculating said microbacterium in a culture medium at a temperature of 25 to 37 ℃, preferably 28 to 30 ℃, most preferably 30 ℃, the pH of said medium being 7.0 to 8.0, preferably 7.4 to 7.8, more preferably 7.6, Optionally, the culture medium is 2216E culture medium, wherein the 2216E culture medium comprises peptone 5.0 g, yeast extract 1.0 g, ferric citrate 0.1 g, sodium chloride 19.45 g, magnesium chloride 5.98 g, sodium sulfate 3.24 g, calcium chloride 1.8 g, potassium chloride 0.55 g, sodium carbonate 0.16 g, potassium bromide 0.08g, strontium chloride 0.034 g, boric acid, 0.022 g, sodium silicate 0.004 g, sodium fluoride 0.0024 g, ammonium nitrate 0.0016 g and disodium hydrogen phosphate 0.008 g in g/L.
  4. 4. A microbial agent comprising the microbacterium of claim 1 or 2 and a microbiologically acceptable adjuvant.
  5. 5. The microbial agent according to claim 5, wherein the microbial agent is in a form selected from the group consisting of wettable powder, water dispersible granules, suspension, aqueous emulsion, granules and combinations thereof.
  6. 6. Use of the microbacterium of claim 1 or 2 or the microbial agent of claim 4 or 5 for promoting the growth of hermetia illucens larvae and/or increasing the accumulation of hermetia illucens protein, fat.
  7. 7. Use of the microbacterium of claim 1 or 2 or the microbial agent of claim 4 or 5 for the conversion of hermetia illucens into organic solid waste.
  8. 8. The method according to claim 7, wherein the organic solid waste comprises wheat bran, kitchen waste, livestock manure, distillers grains, and food processing waste.
  9. 9. Use according to claim 7 or 8, characterized in that it comprises the following steps: (1) Inoculating the microbacterium or the microbial agent into organic solid waste at a ratio of 10 8 cfu/g to obtain a mixed matrix; (2) After the mixed matrix is cultured for 1 day at 28 ℃ and 70% relative humidity, 1200 hermetia illucens larvae at 6 days old are inoculated for continuous culture, so as to obtain biomass.

Description

Novel amylase and cellulase producing microbacterium strain and application thereof in hermetia illucens cultivation and growth promotion Technical Field The application relates to the technical field of microorganisms, in particular to a new strain for producing amylase and cellulase and a new strain for producing cellulase and an application of the new strain in hermetia illucens cultivation and growth promotion. Background With the rapid development of catering industry and large-scale livestock and poultry cultivation in China, the annual production of urban kitchen waste breaks through 1.2 hundred million tons, and the annual production of livestock and poultry manure reaches 38 hundred million tons. The organic solid waste has high water content, is easy to decay, enriches pathogenic bacteria, ova and resistance genes, gives off malodor, breeds mosquitoes and flies if not treated in time, pollutes water along with runoff, causes eutrophication and zoonosis, and becomes a main source of urban and rural surface source pollution. The traditional waste treatment modes such as landfill and incineration have the problems of secondary pollution and resource waste, the composting treatment time is long and is easily influenced by seasons, and the anaerobic fermentation is easy to acidify slag liquid and needs to be reprocessed. Therefore, a new technology for biological treatment of organic solid waste with high efficiency, rapidness, low environmental risk and high product value is needed to break through the bottleneck of the existing technology. As a resource insect, hermetia illucens (Hermetia illucens l.) has been found to have great application potential in environmental management, and its larvae can efficiently take various organic wastes such as kitchen waste, livestock and poultry manure, agricultural waste, etc., and rapidly convert it into own biomass. The process realizes the remarkable reduction and harmlessness of the waste. More notably, the hermetia illucens larvae are rich in protein and fat and are high-quality feed protein sources, so that the recycling and value-added of wastes are realized, and the development concept of recycling economy is met. Some researches show that specific microorganisms can decompose macromolecular organic matters, generate beneficial metabolites and inhibit the growth of pathogenic bacteria, and can theoretically create better intestinal tracts and feed microenvironments for the hermetia illucens, so that the growth and nutrient accumulation of the hermetia illucens, particularly larvae thereof are promoted. Microbacterium is a gram-positive, spore-free, irregular, rod-shaped bacterium formally named 1919 and belongs to the family Microbacteriaceae (Microbacteriaceae) of the phylum actinomycota (Actinomycetota). Microbacteria are widely distributed in soil, water, polar regions, deserts, air and animals and plants, and data are published according to the International Propioneering systems Commission (ICSP) database (https:// lpsn. Dsmz. De /) until month 9 of 2025, the genus microbacteria has been included in 206 species level classification units, of which 168 species are effectively published. At present, the microbacterium has multiple potential of growth promotion stress resistance, pollution degradation, enzyme production pharmacy and film formation corrosion prevention, and becomes a new star microorganism resource for agricultural yield increase, environmental management, medicine development and material protection cross-boundary utilization. In the related art, chinese patent CN118006590B proposes a mutant based on beta-glucosidase from Microbacterium dextranolyticum, the mutant can be used for enzymatic conversion production to improve the production efficiency, and Chinese patent CN108277175B discloses a strain Microbacterium sp.X3 for efficiently degrading 2, 4-dinitrotoluene sulfonate. The strain can efficiently degrade TNT red water and red water polluted soil, has good application prospect in the restoration of nitro compound organic polluted soil, and in addition, chinese patent CN120272385A discloses a dendrobium microbacterium (Microbacterium dendrobii) 2T1 with the functions of siderophore production, potassium decomposition, growth promotion and plant saline-alkali stress alleviation, and the strain is a new strain of Microbacterium and has the capabilities of siderophore secretion and potassium decomposition. Related technology has proved the efficacy of the microbacterium in the fields of growth promotion stress resistance, pollutant degradation, enzyme production pharmacy and the like, and the research on the field of black soldier fly breeding is still blank. The development of the new microbacterium strain capable of promoting the growth of the hermetia illucens and the accumulation of nutrient substances has important significance for promoting the cultivation of the hermetia illucens and the efficient conversion of organic solid wast