CN-121975676-A - Lactobacillus delbrueckii subspecies bulgaricus KFSY10 and application thereof in improving immune function

Abstract

The invention relates to the technical field of microorganisms, and particularly discloses Lactobacillus delbrueckii subspecies bulgaricus KFSY10 and application thereof in improving immune function, wherein the Lactobacillus delbrueckii subspecies bulgaricus KFSY is preserved in the 5 th and 2 nd days of 2018, the preservation address is the China general microbiological culture Collection center, the microbiological research institute of China national academy of sciences No. 3, located in the Guangxi area North Star, beijing, the preservation number is CGMCCNO:15717, and the classification name is Lactobacillus delbrueckii subspecies bulgaricus KFSY10. The Lactobacillus delbrueckii subspecies bulgaricus KSFY10 provided by the invention can directly promote the immune function of an immunosuppressive mouse by protecting and maintaining the normal physiological structure of central and peripheral immune organs and promoting the secretion of serum cytokines and immunoglobulins, and can also indirectly promote the immune function of the mouse by regulating and controlling acute and chronic inflammatory reactions of intestinal tracts, thereby effectively avoiding side effects and adverse reactions caused by using chemical medicines.

Inventors

• ZHAO XIN
• PAN YANNI

Assignees

• 重庆第二师范学院

Dates

Publication Date

20260505

Application Date

20251229

Claims (5)

1. 1. The lactobacillus delbrueckii subspecies bulgaricus KFSY is characterized in that the lactobacillus delbrueckii subspecies bulgaricus KFSY is preserved in the 5 th month 2 of 2018, the preservation address is the China general microbiological center of the culture Collection of microorganisms, and the preservation number is CGMCCNO 15717 and is classified and named as lactobacillus delbrueckii subspecies bulgaricus KFSY10, which is located at the institute of microbiology, national institute of sciences 3, west way 1, the area of facing the sun, beijing.
2. 2. Lactobacillus delbrueckii subsp bulgaricus KFSY10 as claimed in claim 1, wherein the 16S sequence of lactobacillus delbrueckii subsp bulgaricus KFSY10 is shown in SEQ ID No. 1.
3. 3. Use of lactobacillus delbrueckii subsp bulgaricus KFSY10 as claimed in any one of claims 1 to 2 for enhancing immune function.
4. 4. Use of lactobacillus delbrueckii subsp bulgaricus KFSY10 in enhancing immune function according to claim 3, comprising the use of said lactobacillus delbrueckii subsp bulgaricus KFSY and metabolites thereof for the preparation of products for enhancing immune function.
5. 5. The use of lactobacillus delbrueckii subsp bulgaricus KFSY10 for enhancing immune function according to claim 4, wherein the viable bacteria concentration of lactobacillus delbrueckii subsp bulgaricus KFSY10 in said use is 2 x 10 7 CFU/kg-2×10 9 CFU/kg.

Description

Lactobacillus delbrueckii subspecies bulgaricus KFSY10 and application thereof in improving immune function Technical Field The invention relates to the technical field of microorganisms, in particular to lactobacillus delbrueckii subspecies bulgaricus KFSY10 and application thereof in improving immune function. Background The immune system is a strict defense mechanism for resisting pathogen infection, injury and pollutants, and has the functions of identifying and eliminating antigen foreign matters, coordinating with other systems of the body and jointly maintaining the stability and physiological balance of the in-vivo environment. The immune system, the most important protective system of the body, is spread throughout the body and involves a variety of molecules, cells, tissues and organs, a highly complex defense mechanism. Immunosuppression is a transient or long-term immune dysfunction state that can lead to impaired immune surveillance and immune defence functions, disrupting immune homeostasis. Cyclophosphamide (Cyclophosphamide, CTX) can cause immunosuppression in the body and impair immune function, but is widely used as an anticancer agent for treating various cancers because of its anticancer effect, however CTX can interfere with DNA replication, damage mitochondrial and lysosomal membranes, thereby damaging normal tissues, and further leading to increased risk of immunodeficiency and secondary infection. Long-term treatment of CTX may also lead to various side effects such as acute cytotoxicity, immunosuppression and damage to the gastrointestinal mucosal barrier. To improve this situation, some immunoprotectors are increasingly being used to alleviate adverse side effects in patients with chemotherapy. Certain antiparasitic agents have also been reported to have immunomodulatory activity, such as levamisole hydrochloride. Levamisole hydrochloride is a drug widely used to enhance immunity from various human diseases, including leprosy, rheumatoid arthritis, and adjuvant therapy for colorectal cancer. However, intervention of chemical drugs is often accompanied by a degree of adverse reactions and side effects Therefore, lactobacillus delbrueckii subspecies bulgaricus KFSY10 is provided and has important significance in improving the immune function. Disclosure of Invention In view of this, the present invention proposes a lactobacillus delbrueckii subspecies bulgaricus KFSY and its application in enhancing immune function, aiming at solving the problems of adverse reaction and side effects usually accompanied by the intervention of chemical drugs in the above-mentioned background art. In one aspect, the invention provides Lactobacillus delbrueckii subspecies bulgaricus KFSY, wherein Lactobacillus delbrueckii subspecies bulgaricus KFSY is preserved in 2018 on the 5 th month 2 th day, the preservation address is China general microbiological center, the institute of microorganisms of the national academy of sciences 3 of the west way 1 of the Qing dynasty area North Star of Beijing city, the preservation number is CGMCCNO:15717, and the classification name is Lactobacillus delbrueckii subspecies bulgaricus KFSY (Lactobacillus delbrueckii subsp.Bulgaricus). Further, the 16S sequence of Lactobacillus delbrueckii subsp bulgaricus KFSY10 is shown in SEQ ID NO. 1. On the other hand, the invention also provides an application of the Lactobacillus delbrueckii subspecies bulgaricus KFSY10 in improving immune function. Further, the method comprises the application of lactobacillus delbrueckii subspecies bulgaricus KFSY10 and metabolites thereof in preparing products for improving immunity. Further, the viable bacteria concentration of Lactobacillus delbrueckii subsp bulgaricus KFSY in the application is 2X 10 7CFU/kg-2×109 CFU/kg. Compared with the prior art, the Lactobacillus delbrueckii subspecies bulgaricus KSFY has the beneficial effects that the normal physiological structure of central and peripheral immune organs can be protected and maintained, the secretion of serum cytokines and immunoglobulin can be promoted to directly promote the immune function of an immunosuppressive mouse, and the immune function of the mouse can also be indirectly promoted by regulating and controlling acute and chronic inflammatory reactions of intestinal tracts, so that side effects and adverse reactions caused by using chemical medicines can be effectively avoided. Drawings Various other advantages and benefits will become apparent to those of ordinary skill in the art upon reading the following detailed description of the preferred embodiments. The drawings are only for purposes of illustrating the preferred embodiments and are not to be construed as limiting the invention. Also, like reference numerals are used to designate like parts throughout the figures. In the drawings: FIG. 1 is a colony morphology of a strain according to an embodiment of the present invention; FIG. 2 is a graph showing gram stain results