CN-121975681-A - Pseudo-intermediate brucella and application thereof

Abstract

The invention belongs to the technical field of microorganisms, and particularly relates to a pseudo-intermediate Brucella and application thereof. The false middle brucella is deposited in the microorganism strain collection center of Guangdong province in 2025, 3 and 6 days with the preservation number of GDMCC No:65727 and the biological material name of the false middle brucella is Brucella pseudintermedia JQ-1, and the false middle brucella is obtained by separating a strain from a high-temperature Daqu sample, and the strain with good removal capacity for AFB 1 is gram-negative bacillus and has toxin removal capacity for AFB 1 . The removal capacity of the strain on AFB 1 is 89.77%. The addition of Cu 2+ can promote the removal of AFB 1 by fermentation liquor, so that the removal rate is up to 98.68%, and the pseudo-intermediate Brucella is more effective in removing AFB 1 than other strains.

Inventors

• ZHAO YARONG
• WANG XU
• CHEN PEIRONG
• ZENG RUI

Assignees

• 广东省农业科学院农业质量标准与监测技术研究所

Dates

Publication Date

20260505

Application Date

20260115

Claims (10)

1. 1. A pseudo-intermediate brucella, wherein the pseudo-intermediate brucella is deposited in the cantonese province microorganism strain collection center, the deposition date is 2025, 3, 6 and the deposition number is GDMCC No:65727, the biological material name is Brucella pseudintermedia JQ-1, and the taxonomic name is Brucella pseudintermedia; The 16S rRNA gene sequence of the pseudo-intermediate Brucella is shown in a sequence table SEQ ID NO. 1.
2. 2. Use of the pseudo-brucella intermedia of claim 1 in the preparation of a product for removal of mycotoxins.
3. 3. The use according to claim 2, wherein the product comprises a microbial preparation.
4. 4. Use according to claim 2 or 3, wherein the mycotoxin comprises aflatoxin B 1 .
5. 5. A microbial preparation for removing mycotoxins, characterized in that it comprises a pseudo-intermediate brucella strain, a bacterial liquid, a fermentation supernatant, or a metabolite from a fermentation liquid or a fermentation supernatant according to claim 1.
6. 6. The microbial preparation of claim 5, further comprising a metal ion Cu 2 + .
7. 7. A method for preparing a microbial preparation for removing mycotoxins, comprising the steps of: The pseudo-intermediate brucella of claim 1 is cultivated to obtain a seed solution, and then inoculated into a fermentation medium for fermentation to obtain a fermentation broth.
8. 8. The method for preparing a microbial preparation according to claim 7, wherein the cultivation conditions are pH 6.0 to 9.0,30 to 40 ℃ and 150 to 200rpm, and the cultivation is carried out for 24 to 72 hours.
9. 9. The method for preparing a microbial preparation according to claim 7, wherein the fermentation conditions are pH 6.0-9.0, 30-40 ℃, 150-200 rpm, and culturing for 24-72 hours.
10. 10. The method for producing a microbial preparation according to claim 7, further comprising adding Cu 2+ as a metal ion to the fermentation liquid.

Description

Pseudo-intermediate brucella and application thereof Technical Field The invention belongs to the technical field of microorganisms, and particularly relates to pseudo-intermediate Brucella and application thereof. Background Aflatoxin (Aflatoxins, AFs) is a difuranocoumarin derivative which is mainly produced by Aspergillus fungi such as Aspergillus flavus (Aspergillus flavus), aspergillus parasiticus (Aspergillus parasiticus) and the like through metabolism of polyketone biosynthesis, has strong toxicity, carcinogenicity and wide environmental distribution characteristics, and is a mycotoxin pollutant which is focused in the fields of agricultural production and food quality safety. Up to now, more than 20 aflatoxins have been isolated and identified, and aflatoxin B 1, aflatoxin B 2, aflatoxin G 1 and aflatoxin G 2 are common, wherein aflatoxin B 1 is most widely distributed and has strongest toxicity, and is listed as a class I chemical carcinogen by the International cancer research center (International Agency for Research on Cancer, IARC). A large number of in-vitro and in-vivo toxicology studies prove that the carcinogenicity and immunosuppression toxicity of the aflatoxin B 1 to animal organisms are most remarkable in all aflatoxins, and the toxicity is about 10 times of potassium cyanide (KCN) and 68 times of arsenic trioxide (As 2O3). the prior known aflatoxin B 1 removal method mainly comprises physical, chemical, biological and other methods, wherein a physical adsorption method and a chemical degradation method are the main means at present, but the problems of low efficiency, limited large-scale application, secondary pollution and the like possibly exist, and the microbial degradation method removes the aflatoxin B 1 through microbial metabolism of strains and the like, and has the advantages of simple operation, avoiding food nutrition loss, reducing the content of the aflatoxin B 1, short period, high safety, strong specificity, environmental protection and the like. As reported, most of the microorganisms for removing aflatoxin B 1 are yeasts and lactic acid bacteria, and less researches are conducted on Brucella pseudointermedia. Disclosure of Invention In view of the above problems, an object of the present invention is to provide a pseudo-intermediate brucella and its use. The technical content of the invention is as follows: The invention provides a pseudo-intermediate Brucella which is preserved in the microorganism strain preservation center of Guangdong province, the preservation date is 2025, 3, 6 and the preservation number is GDMCC No:65727, the biological material name is Brucella pseudintermedia JQ-1, and the taxonomic name is Brucella pseudintermedia; The 16S rRNA gene sequence of the pseudo-intermediate Brucella is shown in a sequence table SEQ ID NO. 1. The pseudo-middle Brucella is a strain separated from a high-temperature Daqu sample, is in a short rod shape, is gram-negative bacillus, and is named pseudo-middle pallidum (Ochrobactrum pseudintermedium) as an alien strain. The invention also provides application of the pseudo-intermediate brucella in preparing a product for removing mycotoxin; The mycotoxins include aflatoxin B 1. The product comprises a microbial preparation; The mycotoxins include aflatoxin B 1. The invention also provides a microbial preparation for removing mycotoxin, which comprises the pseudo-intermediate Brucella strain, bacterial liquid, fermentation supernatant or active substances (such as extracellular enzymes and the like) such as metabolites of the fermentation liquid or the fermentation supernatant; the microbial preparation also comprises a metal ion Cu 2+. The invention also provides a preparation method of the microbial preparation for removing mycotoxin, which comprises the following steps: Culturing pseudo-intermediate brucella to obtain seed liquid, and inoculating the seed liquid into a fermentation medium for fermentation to obtain fermentation liquid; the cultivation conditions are as follows: a pH of 6.0 to 9.0, a temperature of 30 to 40 ℃, culturing at 150-200 rpm for 24-72 h; The fermentation conditions are as follows: a pH of 6.0 to 9.0, a temperature of 30 to 40 ℃, culturing at 150-200 rpm for 24-72 h; the microbial preparation also comprises a metal ion Cu 2+. The beneficial effects of the invention are as follows: The pseudo-intermediate Brucella is a strain separated from a high-temperature Daqu sample, is gram-negative bacillus, has the removal capacity for aflatoxin B 1, can remove up to 89.77% of aflatoxin B 1 by optimizing culture conditions, and can promote the removal of aflatoxin B 1 by fermentation liquor by adding Cu 2+ on the basis, so that the removal rate is up to 98.68%. The strain provided by the invention is more effective in removing aflatoxin B 1 than other strains. Drawings FIG. 1 is a gram stain of pseudo-Brucella intermedia of the present invention; FIG. 2 shows the colony morphology of pseudo-B