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CN-121975683-A - Rhodophylline anaerobic degradation bacterium and application thereof

CN121975683ACN 121975683 ACN121975683 ACN 121975683ACN-121975683-A

Abstract

The application relates to the technical field of environmental microorganisms, in particular to an anaerobic degradation bacterium for rhodophycoerythrin and application thereof. The strain screened by the application can grow and degrade and remove the rhodophycoerythrin under the conventional condition by taking the rhodophycoerythrin as the sole carbon source, does not need to add other carbon sources, does not produce secondary pollution, has tolerance to the toxicity of the rhodophycoerythrin, has high salt and alkali resistance and good dissolved oxygen adaptability, can survive under the aerobic condition, has stronger degradation activity to the rhodophycoerythrin under the anaerobic condition, and is suitable for the water body polluted by the rhodophycoerythrin. In addition, a screening bacterium source is provided for degrading the rhodophycoerythrin by microorganisms.

Inventors

  • DU MIAOMIAO
  • WANG LIANG
  • HE FEI
  • CAI SIXIN
  • WANG YULING
  • Lv Qingxia

Assignees

  • 浙江工贸职业技术学院(浙江工贸技师学院)

Dates

Publication Date
20260505
Application Date
20260121

Claims (10)

  1. 1. The anaerobic rhodophycoerythrin degrading bacterium is nitro-phenol lemon coccus (Citricoccus nitrophenolicus) and is preserved in China general microbiological culture Collection center (CGMCC) No.36625, with the preservation number of CGMCC No.36625, and the method is characterized in that the anaerobic rhodophycin degrading bacterium is nitro-phenol lemon coccus (Citricoccus nitrophenolicus) which is preserved in China general microbiological culture Collection center (China general microbiological culture Collection center) for type culture collection of 11 th month and 14 th year of 2025.
  2. 2. Use of the anaerobic rhodopsin degrading bacteria according to claim 1 for rhodopsin degradation.
  3. 3. A rhodopsin degrading agent comprising the anaerobic rhodopsin degrading bacterium according to claim 1.
  4. 4. A method for degrading rhodopsin according to claim 1, wherein the anaerobic rhodopsin degrading bacteria are used.
  5. 5. A method for degrading rhodopsin according to claim 4, comprising the step of performing biodegradation by co-culturing a bacterial solution containing the anaerobic rhodopsin degrading bacterium according to claim 1 with rhodopsin under anaerobic conditions.
  6. 6. The method for degrading rhodopsin according to claim 5, wherein the preparation process of the bacterial liquid containing the anaerobic rhodopsin degrading bacteria according to claim 1 comprises the following steps: inoculating the rhodopsin anaerobic degradation bacteria in the first culture medium under anaerobic conditions, and culturing under anaerobic conditions to obtain a culture solution; The culture solution is subjected to centrifugation, washing with a second medium and centrifugation to obtain a bacterial solution containing the anaerobic rhodopsin degradation bacteria according to claim 1.
  7. 7. The method for degrading rhodopsin according to claim 6, wherein the first medium is 2216E liquid medium, and the culture is activated at 20-30 ℃ for 48-96 h after the inoculation of the first medium.
  8. 8. The method of degrading kainic acid according to claim 6 wherein the second medium is a sterilized inorganic salt liquid medium.
  9. 9. The method for degrading rhodopsin according to claim 5, wherein the bacterial liquid and rhodopsin are co-cultured in an inorganic salt liquid medium with a salinity of 3.5% and a pH of 8.
  10. 10. The method for degrading rhodophycali according to claim 9, wherein the bacterial liquid is inoculated into an inorganic salt liquid culture medium according to a bacterial inoculation amount with a volume fraction of 5%.

Description

Rhodophylline anaerobic degradation bacterium and application thereof Technical Field The application relates to the technical field of environmental microorganisms, in particular to an anaerobic degradation bacterium for rhodophycoerythrin and application thereof. Background With the continuous aggravation of the problems of climate warming and sea water eutrophication, red tide disasters are spread worldwide. The toxigenic algae secrete algae toxins during the outbreak of red tide, and 5 to 50 thousands of people are poisoned by eating marine products polluted by the algae toxins by mistake each year, and the global total death rate is 1.5 percent. Rhodophycin (KAINIC ACID, KA) is an algae toxin produced by red algae, and has a structure similar to that of alginic acid, and belongs to strong excitatory neurotoxin. As the binding strength of the rhodophycoerythrin and the glutamate receptor is more than 30 times higher than that of the glutamate receptor, the rhodophycoerythrin and the glutamate receptor can be strongly bound with the glutamate receptor in the brain, thereby causing the loss, damage and death of cell functions, inducing epilepsy and hypomnesis, and being capable of being biologically enriched and biologically amplified in higher organisms along with ocean food chains, and causing serious harm to marine organisms and human beings. Currently, the degradation behavior of kainic acid in marine environments is mainly focused on photodegradation studies. The photodegradation half-life of KA toxin in seawater is longer than that of alginic acid at the same concentration by about 3 times. In addition, the surface layer of the eutrophic seawater in the red tide outbreak process is covered with a large amount of algal bloom and higher phytoplankton to prevent sunlight from transmitting, and the KA photodegradation effect is greatly weakened. Therefore, it is important to find a technology for effectively degrading rhodopsin in the environment. Compared with physical and chemical methods, the method for degrading the rhodophycoerythrin by the microorganism has the advantages of environmental friendliness, low cost, remarkable effect and the like, but no strain which grows by taking the rhodophycoerythrin as the only carbon source and efficiently degrades the rhodophycoerythrin is found at present. Disclosure of Invention Aiming at the defects of the prior art, the application provides an anaerobic degradation bacterium for rhodophycoerythrin and application thereof. To achieve the above object, according to a first aspect of the present application, there is provided an anaerobic rhodopsin degrading bacterium which is a nitrophenol lemon coccus (Citricoccus nitrophenolicus) deposited at the China general microbiological culture collection center (CGMCC) No.36625 at the date of 11/14 of 2025. In a second aspect of the application there is provided the use of a rhodopsin anaerobic degrading bacterium as described above for the degradation of rhodopsin. In a third aspect of the present application, there is provided a rhodopsin degrading agent comprising a rhodopsin anaerobic degrading bacterium as described above. In a fourth aspect of the present application, there is provided a method for degrading rhodopsin by using the anaerobic rhodopsin degrading bacteria as described above. Further, the method comprises the step of performing biodegradation by co-culturing a bacterial solution containing the anaerobic rhodopsin degrading bacteria and rhodopsin under anaerobic conditions. Further, the preparation process of the bacterial liquid containing the rhodopsin anaerobic degradation bacteria comprises the steps of inoculating the rhodopsin anaerobic degradation bacteria into a first culture medium under anaerobic conditions, culturing under anaerobic conditions to obtain a culture liquid, and centrifuging, washing the culture liquid with a second culture medium and centrifuging to obtain the bacterial liquid containing the rhodopsin anaerobic degradation bacteria. Further, the first culture medium is 2216E liquid culture medium, and after being inoculated into the first culture medium, the first culture medium is subjected to activation culture at 20-30 ℃ for 48-96 h. Further, the second culture medium is a sterilized inorganic salt liquid culture medium. Further, the bacterial liquid and the rhodophycoerythrin are co-cultured in an inorganic salt liquid culture medium with the salinity of 3.5% and the pH value of 8. Further, the bacterial liquid is inoculated into an inorganic salt liquid culture medium according to the inoculation amount with the volume fraction of 5 percent. The strain screened by the application can grow and degrade and remove the rhodophycoerythrin under the conventional condition by taking the rhodophycoerythrin as the sole carbon source, does not need to add other carbon sources and does not produce secondary pollution, has tolerance to the toxicity of the rhodophycoerythrin, has h