CN-121975684-A - Application of bacillus H8 in cultivation of herba Duchesneae Indicae

Abstract

The invention belongs to the technical field of plant cultivation, and provides application of bacillus H8 in cultivation of herba Duchesneae Indicae, in particular relates to bacillus H8, which is prepared by preparing bacillus H8 suspension with concentration of 10 2 -10 4 CFU/mL and applying the suspension as biofertilizer to 2-3 cm parts of herba Duchesneae Indicae roots, wherein the suspension is applied once every 7 d parts, so that plant growth can be obviously promoted, and the content of medicinal components of chlorogenic acid and quercetin in leaves is improved.

Inventors

• YAN KAI
• QUAN WENXUAN
• XIONG XIAOQIAN
• LI JIANHONG
• WANG YONG
• ZHANG XIAOYONG
• ZHAO CHAO
• YANG ZHANNAN
• LUO SHIQIONG
• LI CHAOCHAN

Assignees

• 六盘水师范学院
• 贵州师范大学

Dates

Publication Date

20260505

Application Date

20260122

Claims (8)

1. 1. A bacillus H8 is characterized in that the preservation number of the bacillus H8 is CCTCC NO: M20251733.
2. 2. The method of claim 1, wherein the Bacillus subtilis H8 is used for preparing a product for promoting the growth of the herba Duchesneae Indicae and improving the content of the medicinal components.
3. 3. The method for promoting the growth of the red flower and improving the content of the medicinal components of the red flower by using the bacillus H8 according to claim 2 is characterized by comprising the following steps of: S1, selecting yellow soil with loose texture, sufficient nutrients, good drainage property, proper organic matter content and low sand-stone proportion as seedling raising soil; s2, hardening Miao Yizhou the aseptic seedlings of the herba Duchesneae Indicae under natural conditions to obtain cultivation seedlings of the herba Duchesneae Indicae; S3, preparing a bacillus bacterial H8 suspension by using a beef extract peptone agar medium; s4, transferring the small red cultivation seedlings in the S2 into natural soil; S5, applying the bacillus bacterial H8 suspension in the step S3 to roots, and culturing the xiaohong cultivated seedlings in a water and fertilizer management mode until harvesting.
4. 4. The method according to claim 3, wherein the concentration of the H8 suspension in step S3 is 10 2 -10 4 CFU/mL.
5. 5. The method according to claim 3, wherein the application of the H8 suspension to the roots in step S5 is repeated every 7 d until harvest.
6. 6. The method according to claim 2, wherein the pharmaceutical ingredient is at least one of chlorogenic acid or quercetin.
7. 7. The method of claim 2, wherein the product comprises a biofertilizer.
8. 8. The method of claim 6, wherein the biological fertilizer contains Bacillus H8 in an amount of 10 2 -10 4 CFU/mL.

Description

Application of bacillus H8 in cultivation of herba Duchesneae Indicae Technical Field The invention belongs to the technical field of plant cultivation, relates to an xiaoyihong cultivation technology, and particularly relates to application of bacillus H8 in cultivation of xiaoyihong. Background Herba Duchesneae Indicae (Emilia prenanthoidea DC.) is a year of herba Duchesneae Indicae (Emilia) of Compositae (Asteraceae), and is widely distributed in southern area of China, and has long application history in folk medicine. The dry whole herb is used as a medicine, is called thin red back leaf and earpick grass, is an important heat-clearing and detoxifying medicine in Miao medicine systems, and is commonly used for treating swelling and pain of throat, swelling and toxicity of sores, traumatic injury and other symptoms. Modern pharmacological researches show that the herba Duchesneae Indicae contains various active ingredients such as flavonoids, terpenes, phenolic acids and the like, has remarkable anti-inflammatory, anti-tumor, immunoregulation and antioxidation effects, and has higher development value in the aspects of anti-infection, anti-tumor adjuvant therapy and immunoregulation. With the rapid development of the traditional Chinese medicine industry, the demand of the market for the small reddish fruits is continuously increased, and the artificial cultivation becomes a main way for guaranteeing the supply of medicinal materials. In the traditional cultivation process, farmers often rely on chemical fertilizers (such as nitrogen, phosphorus and potassium compound fertilizers) and pesticides to improve the yield, but long-term unreasonable fertilization and medication can lead to problems of soil acidification, microbial community unbalance, heavy metal accumulation and the like, so that soil fertility is reduced, accumulation of active ingredients in medicinal materials can be influenced, and further the quality and clinical curative effect of the medicinal materials are threatened. In addition, excessive application of chemical fertilizer can cause environmental pollution, such as water eutrophication, increased emission of greenhouse gases and the like, and the development concept of green Chinese medicine agriculture is violated. Microbial fertilizers are becoming an important choice for replacing chemical fertilizers due to the advantages of environmental friendliness, strong sustainability and the like. The microbial fertilizer contains specific functional microorganisms (such as rhizosphere growth promoting bacteria PGPR, nitrogen fixing bacteria, phosphate dissolving bacteria and the like), can promote plant growth by means of nitrogen fixation, phosphorus dissolving, secretion of plant hormones (such as IAA, GA and the like) or induction of systemic resistance and the like, improves the absorption and utilization rate of crops to nutrients, and simultaneously enhances the stress resistance (such as drought resistance and disease resistance) of plants. In addition, certain microorganisms can promote the accumulation of effective components such as flavone, polysaccharide, alkaloid and the like in medicinal plants by regulating plant secondary metabolic pathways (such as phenylpropane metabolism, terpene synthesis and the like), so that the medicinal material quality is improved. At present, a certain research has been conducted on the application of microbial fertilizers in the cultivation of medicinal plants, such as inoculation of Arbuscular Mycorrhizal Fungi (AMF) to increase the tanshinone content in the salvia miltiorrhiza bunge. However, the research on the microbial regulation of the small reddish red is still deficient, so that the development of the small reddish red green cultivation technology based on the functional microorganisms has important significance in improving the yield and the quality of the small reddish red, reducing the dependence of chemical fertilizers and promoting the sustainable development of Chinese medicine agriculture. The invention provides a cultivation method for promoting the growth of the herba Duchesneae Indicae and improving the content of medicinal components by inoculating bacteria. The method realizes ecological planting of the herba Duchesneae Indicae by screening the high-efficiency growth promoting strain, provides technical support for high-quality medicinal material production, and promotes the development of Chinese medicine agriculture to the green and high-efficiency directions. Disclosure of Invention The invention aims to provide an application of bacillus in cultivation of herba Duchesneae Indicae, which directly applies bacillus bacterial H8 suspension as a biological fertilizer to the root of herba Duchesneae Indicae, improves the yield of herba Duchesneae Indicae and the content of chlorogenic acid and quercetin medicinal components, and solves the problems of soil degradation, medicinal material quality degradation and unstable cont