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CN-121975687-A - Pectic polysaccharide metabolizing bacteroides fragilis FMBL Ba24006 DMX and application thereof

CN121975687ACN 121975687 ACN121975687 ACN 121975687ACN-121975687-A

Abstract

The invention relates to the technical field of biology, in particular to a pectic polysaccharide metabolizing bacteroides fragilis FMBL Ba24006 DMX and application thereof, which are preserved in China center for type culture collection (CCTCC M2026175) for 1 month and 20 days in 2026, have better pectin metabolism capability, can effectively utilize pectin as a carbon source to realize rapid proliferation, and meanwhile, the strain has good antibacterial effect on hemorrhagic escherichia coli, and is sensitive and moderately sensitive to cefoxitin and rifampicin. The strain can ferment pectin to generate a large amount of short chain fatty acid, and the fermentation liquor taking pectin as a carbon source has strong scavenging ability to DPPH and ABTS free radicals, has good inhibition effect to alpha-amylase activity, can be used for preparing antioxidant products, fermented foods, health care products, food additives and feeds, and has wide application prospect.

Inventors

  • SUN HAILONG
  • NI YONGQING
  • LI XUERUI
  • HUANG LILI
  • ZHANG ZHENGCHUN
  • Zhao Quanhao
  • ZHANG HUIMIN

Assignees

  • 石河子大学

Dates

Publication Date
20260505
Application Date
20260130

Claims (10)

  1. 1. The bacteroides fragilis (Bacteroides fragilis) FMBL Ba24006 DMX for metabolizing pectic polysaccharide is characterized in that the bacteroides fragilis FMBL Ba24006 DMX is preserved in China Center for Type Culture Collection (CCTCC) with the preservation number of CCTCC M2026175 at 1 month 20 of 2026.
  2. 2. A microbial inoculum comprising the Bacteroides fragilis FMBL Ba24006,24006 DMX as claimed in claim 1.
  3. 3. Use of bacteroides fragilis FMBL Ba24006 DMX according to claim 1 or the microbial inoculum according to claim 2 in the preparation of a bacteriostatic product.
  4. 4. Use of bacteroides fragilis FMBL Ba-24006 DMX according to claim 1 or the microbial inoculum according to claim 2 in the preparation of an antioxidant product.
  5. 5. Use of bacteroides fragilis FMBL Ba24006 DMX according to claim 1 or of the microbial inoculum according to claim 2 for the preparation of a pectin metabolizing product.
  6. 6. Use of bacteroides fragilis FMBL Ba-24006 DMX according to claim 1 or a microbial inoculum according to claim 2 in the preparation of a hypoglycemic product.
  7. 7. The preparation of Bacteroides fragilis FMBL Ba24006 DMX according to claim 1 or the microbial inoculum according to claim 2 for inhibiting alpha Use of an active product of a glucosidase.
  8. 8. The preparation of Bacteroides fragilis FMBL Ba24006 DMX according to claim 1 or the microbial inoculum according to claim 2 for inhibiting alpha Use of an active product of an amylase.
  9. 9. Use of bacteroides fragilis FMBL L-24016 DMX or a strain broth thereof according to claim 1 or a microbial inoculum according to claim 2 in the preparation of a food, a food additive, a dietary supplement or a feed additive.
  10. 10. Use of bacteroides fragilis FMBL L-24016 DMX or a strain fermentation broth thereof according to claim 1 or a microbial inoculum according to claim 2 in the preparation of a fermented food.

Description

Pectic polysaccharide metabolizing bacteroides fragilis FMBL Ba24006 DMX and application thereof Technical Field The invention belongs to the technical field of microorganisms, and particularly relates to a pectolysaccharide-metabolizing bacteroides fragilis FMBL Ba24006 DMX and application thereof. Background Dietary fiber is an essential nutrient for maintaining human health, and pectin is a water-soluble dietary fiber with a complex structure, and is paid attention to due to remarkable prebiotic effect and physiological regulation function. Pectin is water-soluble dietary fiber widely existing in plant cell walls and interstitial layers, is particularly rich in plant tissues such as citrus peel, apple pomace and hawthorn pulp, has a chemical structure which presents typical complex polysaccharide characteristics, and is characterized in that a linear main chain formed by connecting D-galacturonic acid monomers through alpha-1, 4-glycosidic bonds forms a basic skeleton of pectin, and various neutral sugar side chains are combined on the main chain, wherein common components comprise rhamnose, arabinose, galactose and the like, and the high complexity and diversity of the structure not only determine the physicochemical properties of the pectin, but also greatly influence the fate and the function of the pectin in an intestinal canal ecosystem. The physiological benefits of pectin have been widely demonstrated, with a central mechanism of action in regulating intestinal microecology and host metabolism. Studies show that pectin can selectively promote the proliferation of beneficial bacteria in intestinal tracts, especially correct dysbacteriosis caused by high-fat diet, and promote the abundance of Short Chain Fatty Acid (SCFAs) producing bacteria such as bifidobacteria, clostridiaceae and the like. These microorganisms ferment and decompose pectin into SCFAs, which are predominantly acetic acid, propionic acid and butyric acid. SCFAs serve as key signaling molecules and energy substances and exert multiple physiological effects, for example, butyric acid is a main energy source of colon epithelial cells and can strengthen intestinal barrier function, propionic acid participates in liver glycolipid metabolism regulation, and acetic acid contributes to systemic metabolic homeostasis. In addition, pectin itself can adsorb cholesterol, delay glucose absorption, and systematically benefit host health by immunomodulation and other means. Bacteroides fragilis is one of the most abundant and studied core symbiotic bacteria in the intestinal tract of humans and many mammals. As a model species of Bacteroides, it belongs to an obligate anaerobic gram-negative bacillus. The existing research shows that the bacteroides fragilis is not only a core member of intestinal microecology, but also a key hub for connecting diet, microbiota and host health, and is a focus of microecology research and conversion medicine. Not only can participate in catabolism of nutrient substances such as carbohydrate, protein and the like to assist in nutrient absorption, but also can reduce the risk of intestinal inflammation by regulating the barrier function of intestinal mucosa and inhibiting the excessive proliferation of pathogenic bacteria, and meanwhile, the metabolic products can assist in regulating the metabolic balance of organisms. However, there are few reports of Bacteroides capable of directly utilizing pectin. The inventor separates a strain of fragile bacillus FMBL Ba24006 DMX from human intestinal tracts, the strain can ferment pectic polysaccharide, can rapidly proliferate in a culture medium taking pectic polysaccharide as a sole carbon source, generates a large amount of short chain fatty acid by fermentation, wherein the total amount of the short chain fatty acid which can be generated in fermentation liquor taking apple pectin as the carbon source is 1037.5 mug/ml, the total amount of the short chain fatty acid which can be generated in fermentation liquor taking orange pectin as the carbon source is 671.5 mug/ml, the clearance rate of the extracellular supernatant of the apple pectin to ABTS and DPPH free radicals respectively reaches 83% and 75%, the clearance rate of the extracellular supernatant of the orange pectin to ABTS and DPPH free radicals respectively reaches 78% and 67%, the inhibition rate of the extracellular supernatant of the apple pectin to alpha-amylase activity is 81.08%, and the inhibition rate of the extracellular supernatant of the orange pectin to alpha-amylase activity is 79%, thereby having good antioxidant and hypoglycemic capabilities. Can be used for preparing antioxidant products, hypoglycemic drugs, fermented foods, health products, food additives and feeds, and has wide application prospect. Disclosure of Invention Aiming at the technical problems, the primary purpose of the invention is to provide a bacteroides fragilis (Bacteroides fragilis) FMBL Ba24006 DMX for metabolizing pectic p