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CN-121975693-A - Bacteroides oval and application thereof in degradation preparation of chondroitin sulfate oligosaccharide

CN121975693ACN 121975693 ACN121975693 ACN 121975693ACN-121975693-A

Abstract

The invention discloses an bacteroides ovatus and application thereof in degrading and preparing chondroitin sulfate oligosaccharides, belonging to the technical field of microorganisms, wherein the bacteroides ovatus JP2025813AP1 is preserved in China general microbiological culture collection center (CGMCC) No. 46908 in the 12 th month 05 of 2025. The invention also provides application of the bacteroides ovatus JP2025813AP1 in degradation preparation of chondroitin sulfate oligosaccharide, and the bacteroides ovatus JP2025813AP1 can degrade the chondroitin sulfate into unsaturated disaccharide.

Inventors

  • ZHU CHANGLIANG
  • HUANG JUNPENG
  • MOU HAIJIN
  • GAO YUHONG

Assignees

  • 中国海洋大学

Dates

Publication Date
20260505
Application Date
20260204
Priority Date
20260203

Claims (8)

  1. 1. An oval bacteroides is characterized in that the oval bacteroides is classified and named Bacteroides ovatus JP2025813AP1, JP2025813AP1 is preserved in China general microbiological culture collection center (CGMCC) with the preservation number of 46908 in 2025 and 12 months 05.
  2. 2. Use of bacteroides ovatus according to claim 1 for the preparation of chondroitin sulfate oligosaccharides by degradation of chondroitin sulfate.
  3. 3. The use according to claim 2, wherein said bacteroides ovatus is capable of degrading chondroitin sulfate to unsaturated chondroitin sulfate oligosaccharides, the product comprising unsaturated disaccharides.
  4. 4. The use according to claim 3, wherein the chondroitin sulfate has a weight average molecular weight of 45.4 kDa.
  5. 5. The use according to claim 4, wherein the bacteroides ovatus is activated and inoculated as a seed solution into a chondroitin sulfate medium for anaerobic fermentation, the fermentation product comprising an unsaturated disaccharide.
  6. 6. The use according to claim 5, wherein the final concentration composition of the chondroitin sulfate culture medium is 8g/L of chondroitin sulfate with weight average molecular weight of 45.4 kDa, 10g/L of pancreatic protein, 2.5g/L of yeast extract, 1g/L of L-cysteine hydrochloride, 0.9g/L of chloridized calcium chloride, 0.09g/L of monopotassium phosphate, 0.45g/L of dipotassium phosphate, 0.09g/L of magnesium sulfate heptahydrate, 0.001g/L of resazurin, 0.01g/L of chlorhydrin, 100 mu L of vitamin mixed solution and pH value of 6.7-6.9.
  7. 7. A biological agent comprising the bacteroides ovatus according to claim 1.
  8. 8. The use of the biological agent of claim 7 in the preparation of chondroitin sulfate oligosaccharides by degrading chondroitin sulfate.

Description

Bacteroides oval and application thereof in degradation preparation of chondroitin sulfate oligosaccharide The patent claims priority of 2026, 2-3 and 202610153376.9 under the name of "a strain of bacteroides ovatus and its application in degradation and preparation of chondroitin sulfate oligosaccharide Technical Field The invention belongs to the technical field of microorganisms, and particularly relates to bacteroides ovatus and application thereof in degradation preparation of chondroitin sulfate oligosaccharides. Background Chondroitin sulfate (Chondroitin sulfate, CS) is one of glycosaminoglycans (GAGs) that has a variety of physiological functions, such as antioxidant, anti-inflammatory, anticoagulant and immunomodulating. In the medical and health care fields, CS is often used as an oral therapeutic drug and nutritional supplement for osteoarthritis. Long-term clinical application shows that the absorption rate of high molecular weight CS in the intestinal tract is low, and the macromolecular structure of CS limits the absorption efficiency of CS through the intestinal wall, resulting in low bioavailability. And CS oligosaccharide can improve the absorptivity and the bioactivity, reduce the dosage and improve the efficacy. Furthermore, CS oligosaccharides may have new functions in vivo that are different from the parent polysaccharide, e.g. more easily involved in cell signaling or better regulate immune responses. Currently, the traditional methods for preparing CS oligosaccharides mainly include chemical hydrolysis and enzymatic hydrolysis. Chemical hydrolysis methods typically utilize conditions such as strong acids, strong bases, or high temperatures to randomly cleave long CS chains into smaller fragments. The method is simple to operate, the reaction condition is harsh, the structure and activity of the sulfuric acid group of CS are easy to damage, the polymerization degree distribution of the produced oligosaccharide is wide, and the impurity is more. Compared with the chemical method, the enzymolysis mode has mild condition and more controllable product structure. However, the cost of purifying enzyme preparations is high, which limits their use in industrial production. In recent years, with the intensive research of human intestinal microorganisms, microbial degradation has become an emerging direction for preparing polysaccharide oligosaccharides. Bacteria of the genus Bacteroides (bacterioides) in the human intestinal flora are known for their strong polysaccharide degrading capacity and play a key role in the breakdown of complex polysaccharides and glycosaminoglycans which are difficult to digest by the host. Some studies have found that specific intestinal bacteria are able to utilize and degrade complex polysaccharides such as chondroitin sulfate. For example, it has been reported that certain species of Bacteroides (bacterioides), such as Bacteroides salvinsis (Bacteroides salyersiae), bacteroides thetaiotaomicron (Bacteroides thetaiotaomicron), and the like, have the ability to ferment and degrade CS, and can cleave CS molecules to produce unsaturated oligosaccharide products. However, there are differences in the efficiency of degrading CS and product distribution from strain to strain, and some strains only partially degrade CS or products, mainly oligosaccharides with a large degree of polymerization. Bacteroides ovatus (Bacteroides ovatus) also has polysaccharide degrading ability, but whether it can degrade chondroitin sulfate is not clearly studied and reported at present. The research discovers for the first time that the Bacteroides ovatus JP2025813AP1 can degrade CS into CS oligosaccharide with the polymerization degree of mainly 2, provides a novel chondroitin sulfate degradation path, and has wide application prospect. Disclosure of Invention Aiming at the technical problems, the invention provides a microorganism strain for efficiently degrading chondroitin sulfate, namely bacteroides ovatus (Bacteroides ovatus) JP2025813AP1 and application thereof in degradation preparation of Chondroitin Sulfate Oligosaccharide (CSOs). The technical scheme adopted by the invention is as follows: The invention provides an oval bacteroides which is classified and named Bacteroides ovatus JP2025813AP1, wherein the oval bacteroides JP2025813AP1 is separated from feces of healthy adult females and is preserved in China general microbiological culture collection center (CGMCC) of China general microbiological culture Collection center (CGMCC) with the preservation number of No. 46908 in month 05 of 2025. The invention also provides application of the bacteroides ovatus JP2025813AP1 in preparing chondroitin sulfate oligosaccharide by degrading chondroitin sulfate. Further, the above-mentioned Bacteroides oval JP2025813AP1 can degrade chondroitin sulfate into unsaturated chondroitin sulfate oligosaccharide, and the polymerization degree of the main product is 2, i.e., unsaturated disaccharid