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CN-121975694-A - Arthrobacter for improving acidic white clay, microbial agent and application thereof

CN121975694ACN 121975694 ACN121975694 ACN 121975694ACN-121975694-A

Abstract

The invention discloses arthrobacter for improving acid white clay, and a microbial agent and application thereof. The Arthrobacter (sp.) W36-1 is preserved in CGMCC with the preservation number of No. 31903. The strain is separated from white serosal soil and is characterized by having high-efficiency biological acid (alkali) reducing capability, and can raise the pH value of a culture medium of 5.0 to 7.66 within 72 hours. Under the condition of acid stress with pH less than or equal to 5.5, the strain can synchronously play multiple functions of dissolving phosphorus, dissolving potassium, fixing nitrogen, producing IAA, EPS and the like. The potting experiment proves that the microbial inoculum can effectively promote the pH of acid white serosal soil, obviously relieve the damage of acid stress to soybeans, promote the root growth of the soybean, increase the root nodule number and biomass, and has good growth promoting effect on rice germination under acid stress.

Inventors

  • WANG NANNAN
  • CAI LIJUN
  • ZHANG ZHENYU
  • MENG XIANGHAI
  • WANG XINYI
  • SONG YINGBO
  • ZHANG JIANPENG
  • FENG HAOYUAN
  • DING JUNJIE
  • LI CANDONG
  • LIU KAI
  • WANG SHUANG

Assignees

  • 黑龙江省农业科学院佳木斯分院

Dates

Publication Date
20260505
Application Date
20260210

Claims (10)

  1. 1. An Arthrobacter sp W36-1 strain for improving acidic white clay is characterized in that the Arthrobacter sp W36-1 belongs to Arthrobacter, arthrobacter and Arthrobacter strains are preserved in China general microbiological culture Collection center (China general microbiological culture Collection center) at 9 months and 19 days of 2024, and the preservation address is number 3 of the West Song No. 1, the Korean region North Star of Beijing, and the preservation number is CGMCC No. 31903.
  2. 2. A microbial agent comprising the Arthrobacter sp according to claim 1 as an active ingredient.
  3. 3. The microbial agent of claim 2, wherein the active ingredient is selected from the group consisting of a bacterial strain, a fermentation broth, a fermentation supernatant, and a mixture thereof.
  4. 4. The microbial agent of claim 2, wherein the microbial agent is formulated as a wettable powder, a water-dispersible, an aqueous suspension, or a dispersible oil suspension, wherein the effective viable count is no less than 1 x 10 8 CFU/g or CFU/mL.
  5. 5. The preparation method of the microbial agent according to any one of claims 2 to 4, which is characterized in that the Arthrobacter W36-1 according to claim 1 is inoculated into a liquid culture medium, and is cultured in a shake flask with the temperature of 27 to 30 ℃ and the rotation speed of 150 to 200r/min, and when the strain grows to the logarithmic phase, sterile water or culture medium is used for diluting the bacterial liquid, so that the microbial agent is obtained.
  6. 6. Use of the arthrobacter W36-1 of claim 1 or the microbial agent of claim 2 in any of the following aspects: (1) The application in preparing a soil conditioner for improving the pH value of acid soil with the pH value not higher than 5.5; (2) The application of the soybean root system inhibitor in preparing a preparation for relieving acid stress of soybean in acid soil, promoting root system development and increasing root nodule number is provided.
  7. 7. The use according to claim 6, wherein the acid soil is white clay.
  8. 8. Use of the arthrobacter W36-1 of claim 1 or the microbial agent of claim 2 in the preparation of a formulation for alleviating acid stress on rice in acid soil having a pH of not more than 5.5 and promoting seed germination thereof.
  9. 9. A method of modifying an acidic white clay, characterized in that an effective amount of the arthrobacter W36-1 of claim 1 or the microbial agent of claim 2 is applied to the acidic white clay.
  10. 10. A soil conditioner comprising the arthrobacter W36-1 of claim 1 or the microbial agent of claim 2.

Description

Arthrobacter for improving acidic white clay, microbial agent and application thereof Technical Field The invention relates to the technical field of microorganisms, in particular to a novel arthrobacter strain which is separated from white serosal and has high-efficiency biological acid (alkali) reducing and acid stress relieving functions, a microbial agent thereof and application thereof in improving acid soil, in particular to relieving the acid damage of white serosal and promoting the growth of crops under acid stress. Background The disclosure of this background section is only intended to increase some understanding of the general background of the invention and is not necessarily to be construed as an admission or any form of suggestion that this information forms the prior art already known to those of ordinary skill in the art. Soil degradation is a global challenge that limits sustainable development of agriculture, while soil acidification is one of the most harmful and difficult barriers. When acidizing is combined with a specific undesirable soil structure, its hazard is exponentially amplified. White serosity soil widely distributed in the plain of three rivers in northeast of China is a typical representative of the type of barrier acidified soil, and the problem of low yield is a systematic problem formed by interweaving physical, chemical and biological factors. First, white clay has tough physical structure defects. The white pulp layer in the section has the advantages of heavy texture, compact structure and extremely poor water permeability, which not only causes the frequent occurrence of the flood and drought disasters, but also severely limits the penetration and expansion of root systems. Second, the more critical and immediate limitation comes from its chemical environment, especially soil acidification. A substantial proportion of the white clay exhibits significant acidity, with pH values often below 5.5. This acidic environment is not isolated and triggers a series of linked toxic and inhibitory effects: Direct ion poisoning, namely high-concentration H + damages the cell membrane of the root system. Meanwhile, the acidic condition strongly activates active aluminum (Al 3+) and manganese (Mn 2+) plasmas in soil, and the plasmas have direct toxic action on plant root systems, particularly root tip meristems, so that root system development is seriously inhibited. The biological effectiveness of the nutrients is suddenly reduced, namely, the nutrient elements such as phosphorus, calcium, magnesium and the like which are necessary for the plants in the soil are fixed under the acidic condition and are converted into a form which is difficult to absorb, so that the nutrients are in the soil, but the contradiction situation that the plants cannot absorb is caused. The damaged root system further weakens the absorption capacity of the plant to moisture and nutrient, resulting in poor crop growth and reduced stress resistance. Soil acidification, aluminum-manganese activation/nutrient fixation, root damage, absorption disorder and low yield of crops form a vicious circle which is difficult to break. Thus, remediation of acid hazards is a primary and central chemical task in breaking this cycle, improving such soil. In recent years, environmentally friendly microbial remediation techniques have been considered as potential approaches to improving degenerated soil. In the prior art, there have been reports related to the use of Arthrobacter in agriculture. For example, there are patents disclosing the use of Arthrobacter for high temperature composting to degrade straw and pesticides (e.g. CN 112725213B), and another patent disclosing a Arthrobacter strain which is salt-tolerant, acid-base-resistant and has a phosphate-solubilizing function, which was developed mainly as a biological seed coating compatible with chemical seed coatings, and reporting its pro-effectiveness in neutral to slightly alkaline soil (e.g. CN 112501086B). However, through intensive analysis, the prior art approaches still face some specific challenges in purposefully solving the core symptoms of barrier acidified soil (e.g., acidity Bai Jiangtu), namely to efficiently and actively reverse soil acidification process and achieve crop growth promotion in this adversity: In particular, the published data for existing acid-tolerant strains (e.g., CN 112501086B) focused on their ability to grow (tolerance) over a wide pH range and on the phosphate-solubilizing effect under neutral conditions. However, the prior disclosures do not provide clear verification or suggestion as to whether it can actively and effectively raise the pH (i.e., bio-deacidification or alcaligenes) of the environment in a strongly acidic (pH < 5.5) environment, which is a key metabolic capability for directly alleviating acid damage. Meanwhile, in a strong acid stress environment, microorganisms need to stably and cooperatively play multiple funct