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CN-121975697-A - Multifunctional lactobacillus plantarum HR65 and application thereof

CN121975697ACN 121975697 ACN121975697 ACN 121975697ACN-121975697-A

Abstract

The invention relates to the technical field of microorganisms, and discloses a lactobacillus plantarum (Lactiplantibacillus plantarum) HR65 with a preservation number of CGMCC No.35607. The strain can ferment various saccharide compounds for growth, has remarkable effects in removing peculiar smell of traditional Chinese medicine and improving flavor, and can especially reduce the content of bad flavor substances such as 1,3, 5-trithiane, 3-phenyl-2-acrolein and the like in the spleen nourishing powder. The fermented spleen-nourishing powder and purslane can obviously improve the content of organic acids and polypeptides such as lactic acid, 2-hydroxy-3-methylbutyric acid and the like, and the fermented spleen-nourishing powder can also improve the level of short-chain fatty acids such as acetic acid, butyric acid and the like. The strain has the characteristics of bacteriostasis, antioxidation, SOD enzyme production, cholesterol reduction, self aggregation and hydrophobicity, can widely ferment the matrixes such as carrots, roxburgh rose, various fruits, chinese herbal medicines, grains, milk and the like, improves the flavor and the quality of the matrixes, and has good application value in the development of plant-based fermentation products, traditional Chinese medicine flavor improvement, functional foods and probiotics preparations.

Inventors

  • GUAN XUEFANG
  • Zhong Lianyue
  • WANG QI

Assignees

  • 福建省农业科学院农产品加工研究所

Dates

Publication Date
20260505
Application Date
20260210

Claims (10)

  1. 1. The plant lactobacillus plantarum HR65 is characterized in that the plant lactobacillus plantarum HR65 is preserved in the China general microbiological culture Collection center of the China Committee for culture Collection of microorganisms on 8 th month 13 of 2025, and is classified and named as plant lactobacillus plantarum (Lactiplantibacillus plantarum), the preservation number is CGMCC No. 35607, and the preservation address is North Star Xiyu No. 1, 3 in the Chaiyang area of Beijing city.
  2. 2. The application of the lactobacillus plantarum HR65 in removing traditional Chinese medicine bad flavor substances, which is characterized in that the lactobacillus plantarum HR65 is inoculated into a fermentation substrate containing traditional Chinese medicine components for fermentation to obtain a fermentation product with the content of 1,3, 5-trithiane and/or 3-phenyl-2-acrolein lower than that of the fermentation substrate, wherein the fermentation substrate containing the traditional Chinese medicine components comprises spleen nourishing powder.
  3. 3. The use of lactobacillus plantarum HR65 according to claim 1 for the preparation of an organic acid rich product, wherein lactobacillus plantarum HR65 is inoculated into a fermentation substrate for fermentation to obtain a fermentation product with a higher content of lactic acid, 2-hydroxy-3-methylbutyric acid, DL-3-phenyllactic acid, 3- (4-hydroxyphenyl) lactic acid, pyruvic acid and/or 3-indoleacetic acid than the fermentation substrate, wherein the fermentation substrate comprises Yangpi san or purslane.
  4. 4. The use of lactobacillus plantarum HR65 according to claim 1 for the preparation of products enriched in short chain fatty acids, wherein lactobacillus plantarum HR65 is inoculated into a fermentation substrate for fermentation to obtain a fermentation product having a higher content of acetic acid, isobutyric acid, butyric acid, isovaleric acid, valeric acid and/or caproic acid than the fermentation substrate, wherein the fermentation substrate comprises a spleen nourishing powder.
  5. 5. The use of the plant lactobacillus HR65 according to claim 1 for preparing a polypeptide-rich product, wherein the plant lactobacillus HR65 is inoculated into a fermentation substrate for fermentation to obtain a fermentation product with a polypeptide content higher than that of the fermentation substrate, and the fermentation substrate comprises spleen nourishing powder or purslane.
  6. 6. The use of the plant lactobacillus HR65 according to claim 1 for the preparation of a bacteriostatic agent, wherein said bacteria comprise Streptococcus mutans, staphylococcus aureus and/or Shigella sonnei.
  7. 7. The application of the lactobacillus plantarum HR65 in preparing an antioxidant product, which is characterized in that the lactobacillus plantarum HR65 is inoculated into a fermentation substrate for fermentation to obtain a fermentation product with higher total antioxidant capacity and/or higher superoxide dismutase activity than the fermentation substrate, wherein the fermentation substrate comprises MRS culture medium, spleen nourishing powder or ginseng.
  8. 8. The use of the plant lactobacillus HR65 for reducing cholesterol according to claim 1, wherein the plant lactobacillus HR65 is inoculated in a MRS liquid culture medium containing cholesterol for culture.
  9. 9. The use of Lactobacillus plantarum HR65 according to claim 1for preparing a fermented food, wherein the fermented food is prepared from radix Dauci Sativae, fructus Vitis Viniferae, fructus Rosae Normalis, herba Avenae Fatuae, YANGPISAN, herba Portulacae, fructus Lycii Frutus, radix Ginseng, milk, semen Coicis or herba Avenae Fatuae.
  10. 10. A fermented product is characterized by being prepared from the lactobacillus plantarum HR65 fermentation raw material according to claim 1, wherein the raw material is selected from carrot, grape, roxburgh rose, oat, spleen nourishing powder, purslane, red matrimony vine, ginseng, milk, pearl barley or oat.

Description

Multifunctional lactobacillus plantarum HR65 and application thereof Technical Field The invention relates to the technical field of microorganisms, in particular to a multifunctional lactobacillus plantarum HR65 and application thereof. Background The spleen nourishing powder is used as a historical famous medicine in Quanzhou of Fujian province, and the core components of the spleen nourishing powder comprise various traditional Chinese medicinal materials such as dangshen, bighead atractylodes rhizome, poria cocos, lotus seeds, cinnamon, fructus amomi, clove, cardamom, liquorice and the like. However, the inherent bitter taste and herbal smell of traditional Chinese medicines are more prominent, and the product forms are powder, low in eating convenience and other factors, so that the market sales of the traditional Chinese medicines are extremely tired. Purslane (commonly called as 'longevity vegetable') is a plant with homology of medicine and food, is traditionally used for clearing heat and detoxicating, and modern research reveals rich nutritional value and medical potential. It is rich in vitamins A, B, C and E, has high protein content, and contains Omega-3 fatty acid, flavone, polysaccharide, alkaloid, and minerals such as potassium, calcium, and iron. Has antibacterial and antiinflammatory effects, and is used for treating eczema and gastroenteritis, regulating blood sugar and blood pressure, protecting heart, resisting oxidation and aging. However, purslane is mostly eaten in fresh food and dry samples, and the eating convenience is low, so that the development of the industry is hindered. In order to solve the problems, lactobacillus strains with good effect of removing the medicinal odor are screened from natural fermentation environment of the spleen nourishing powder, the fermentation effect of the lactobacillus strains on various substances such as purslane is measured, and a high-quality fermentation bacteria source is provided for the spleen nourishing powder, the purslane, the fermented milk, the carrot, the coix seed, the oat, the rest fruits and the traditional Chinese medicinal materials. Disclosure of Invention In order to solve the defects of the prior art mentioned in the background art, the invention aims to provide a multifunctional lactobacillus plantarum HR65 and application thereof. In order to achieve the above purpose, the invention adopts the following technical scheme: The plant lactobacillus plantarum HR65 is preserved in China general microbiological culture Collection center (Lactiplantibacillus plantarum) of China Committee for culture Collection of microorganisms on 8 and 13 days of 2025, and is classified and named as lactobacillus plantarum (Lactiplantibacillus plantarum), wherein the preservation number is CGMCC No. 35607, and the preservation address is North Star Xiyang area of Beijing city, no.1 and No. 3. The application of the lactobacillus plantarum HR65 in removing the traditional Chinese medicine bad flavor substances comprises the steps of inoculating the lactobacillus plantarum HR65 into a fermentation substrate containing traditional Chinese medicine components for fermentation to obtain a fermentation product with the content of 1,3, 5-trithiane and/or 3-phenyl-2-acrolein lower than that of the fermentation substrate, wherein the fermentation substrate containing the traditional Chinese medicine components comprises spleen nourishing powder. The application of the lactobacillus plantarum HR65 in preparing the organic acid-rich product comprises the steps of inoculating the lactobacillus plantarum HR65 into a fermentation substrate for fermentation to obtain a fermentation product with the content of lactic acid, 2-hydroxy-3-methylbutyric acid, DL-3-phenyllactic acid, 3- (4-hydroxyphenyl) lactic acid, pyruvic acid and/or 3-indoleacetic acid higher than that of the fermentation substrate, wherein the fermentation substrate comprises spleen nourishing powder or purslane. The application of the lactobacillus plantarum HR65 in preparing the short-chain fatty acid-rich product comprises the steps of inoculating the lactobacillus plantarum HR65 into a fermentation substrate for fermentation to obtain a fermentation product with the content of acetic acid, isobutyric acid, butyric acid, isovaleric acid, valeric acid and/or caproic acid higher than that of the fermentation substrate, wherein the fermentation substrate comprises spleen nourishing powder. The application of the lactobacillus plantarum HR65 in preparing the polypeptide-rich product comprises the steps of inoculating the lactobacillus plantarum HR65 into a fermentation substrate for fermentation to obtain a fermentation product with the polypeptide content higher than that of the fermentation substrate, wherein the fermentation substrate comprises spleen nourishing powder or purslane. The application of the lactobacillus plantarum HR65 in preparing a bacteriostatic agent comprises streptococcus mut