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CN-121975698-A - Halophilic aeromonas Aeromicrobium sp.SZW-1, separation method thereof and application thereof in plastic degradation

CN121975698ACN 121975698 ACN121975698 ACN 121975698ACN-121975698-A

Abstract

The invention discloses a halophilic aeromonas Aeromicrobium sp.SZW-1, a separation method thereof and application thereof in plastic degradation. The halophilic aerobe Aeromicrobium sp.SZW-1 with the preservation number of GDMCC No:67081.Aeromicrobium sp.SZW-1 has a tolerance NaCl concentration range of 0-0.15 g/mL, an optimal NaCl concentration of 0.03 g/mL, and has hydrolytic activity on PET. The strain has the advantages of natural adaptability to high-salt environment and PET degradation activity, provides key technical materials and solutions for PET pollution treatment, particularly for high-salt environment treatment which is difficult to reach by the traditional biotechnology, and also has obvious environmental protection value and application potential.

Inventors

  • ZHOU XIAOLI
  • ZHU HONGHUI
  • LI XIAN
  • DENG MINGRONG

Assignees

  • 广东省科学院微生物研究所(广东省微生物分析检测中心)

Dates

Publication Date
20260505
Application Date
20260211

Claims (9)

  1. 1. A halophilic aeromonas Aeromicrobium sp.SZW-1 is characterized by having a preservation number of GDMCC No:67081.
  2. 2. A microbial agent comprising the microorganism cell and/or the metabolite thereof of claim 1, wherein the microorganism cell comprises Aeromicrobium sp.
  3. 3. The microbial agent of claim 2, wherein the microbial agent is a fermentation broth of halophilic aero microorganisms Aeromicrobium sp.
  4. 4. The microbial agent according to claim 3, wherein the fermentation broth is obtained by inoculating Aeromicrobium sp.SZW-1 to LB medium containing 0.03: 0.03 g/mL NaCl, shake culturing at 37 ℃ and removing the thallus.
  5. 5. Use of a halophilic aero microorganism Aeromicrobium sp, SZW-1 according to claim 1 or a microbial agent according to any one of claims 2-4 in plastics degradation and conversion.
  6. 6. The use according to claim 5, wherein the plastic is polyethylene terephthalate.
  7. 7. Use according to claim 6, characterized in that the polyethylene terephthalate degradation products comprise terephthalic acid and mono (2-hydroxyethyl) terephthalate.
  8. 8. A method for degrading and converting plastics, comprising the step of degrading plastics using the halophilic aeromonas Aeromicrobium sp.szw-1 of claim 1 or the microbial agent of any one of claims 2-4.
  9. 9. The method of claim 8, wherein the plastic is polyethylene terephthalate.

Description

Halophilic aeromonas Aeromicrobium sp.SZW-1, separation method thereof and application thereof in plastic degradation Technical Field The invention belongs to the technical field of biodegradation, and particularly relates to a halophilic aeromonas Aeromicrobium sp, SZW-1, a separation method thereof and application thereof in plastic degradation. Background Polyethylene terephthalate (PET) plastics are widely used because of their excellent physicochemical properties, and their wastes have constituted global "white pollution", especially PET plastics that enter the marine environment, which have been difficult to degrade naturally and exist for a long time and are broken into microplastic, pose a serious threat to the ecosystem and human health. Biodegradation, particularly with microorganisms or enzymatic polymerization of PET, is considered an environmentally friendly green solution. At present, although several strains, including Ideonella sakaiensis, have been found to possess PET degrading activity, their practical use still faces a significant bottleneck. These conventional strains and their enzyme preparations generally need to function under mild conditions, and their biological processes are extremely susceptible to contamination by infectious agents and have extremely poor adaptability to extreme environments such as high salt, high permeability, etc. This makes them not directly applicable to high-salt industrial wastewater rich in PET microplastic, and makes it more difficult to treat marine plastic pollution in situ. The method has the advantages that microorganisms in environments such as seawater have natural adaptability to high-salt environments, on one hand, seawater or industrial high-salt wastewater can be directly used as a culture medium, expensive pretreatment is not needed, in-situ and low-cost biological removal of PET pollution in a salt-containing medium is realized, a brand-new technical path is provided for marine plastic pollution and special industrial wastewater treatment, on the other hand, the high-salt adaptability can create a natural sterile operation environment, the bacteria contamination risk and the sterilization cost in the fermentation or biological treatment process are obviously reduced, and the process robustness and economy are improved. Therefore, the mining of PET degradation strains that can adapt to severe environments such as high salt becomes an urgent need to solve the above-mentioned problem of specific scene pollution. Disclosure of Invention The first object of the present invention is to provide a halophilic aerobe Aeromicrobium sp.SZW-1 deposited at the microorganism strain collection (GDMCC) of Guangdong province at 10.13.2025, address: building 5, post code: 510070, accession number: GDMCC No:67081, 30.5, 30 th university, martyr th, guangzhou province, guangdong. The second object of the present invention is to provide a microbial agent comprising the microorganism cells of the halophilic aeromonas Aeromicrobium sp.szw-1 and/or a metabolite thereof. Preferably, the microbial agent is fermentation broth of aeromonas aerophila aeromonas sp. Further preferably, the fermentation broth is obtained by inoculating SZW-1 to LB culture containing 0.03 g/mL NaCl, shake culturing at 37 ℃ and removing thalli. The third object of the invention is to provide the application of the halophilic aero microorganism Aeromicrobium sp, SZW-1 or the microbial agent in plastic degradation and conversion. Preferably, the plastic is polyethylene terephthalate (PET). Further preferred polyethylene terephthalate (PET) degradation products include terephthalic acid (TPA) and mono (2-hydroxyethyl) terephthalate (MHET). The fourth object of the invention is to provide a plastic degradation and transformation method, which comprises the step of degrading plastic by using the halophilic aeromonas Aeromicrobium sp. Preferably, the plastic is polyethylene terephthalate (PET). The beneficial effects of the invention are as follows: The strain SZW-1 is a halophilic aerobe, the optimal growth temperature is 30-37 ℃, the concentration range of tolerant NaCl is 0-0.15 g/mL, the optimal NaCl concentration is 0.03 g/mL, and the strain has hydrolytic activity on PET. The strain has the advantages of natural adaptability to high-salt environment and PET degradation activity, provides key technical materials and solutions for PET pollution treatment, particularly for high-salt environment treatment which is difficult to reach by the traditional biotechnology, and also has obvious environmental protection value and application potential. Halophilic aerobe Aeromicrobium sp.SZW-1 deposited at the microorganism strain collection (GDMCC) of Guangdong province on day 13 of 10 of 2025, at floor 5 of the university of road 100, no. 59, in Guangzhou city martyr of Guangdong province, post code 510070, accession number GDMCC No:67081. Drawings FIG. 1 shows a method for isolating strain SZW-1 in exam