CN-121975701-A - Marine bacterium JHS7 and application thereof

Abstract

The invention discloses marine bacteria JHS7 and application thereof, and belongs to the technical field of microorganisms. The marine bacteria JHS7 provided by the invention is used for separating and screening natural seawater environment of the sea area of the national smoke table golden beach, the strain can efficiently and directionally degrade oyster polysaccharide, the relative molecular mass distribution of degradation products is relatively concentrated, under the conditions that the growth temperature is 30 ℃, the salinity is 3%, the pH value is 8.0 and the inoculation amount is 3%, the degradation rate of the strain on the oyster polysaccharide reaches 58.06%, and the relative molecular mass distribution range of the degradation products is within 1100Da, so that the oyster oligosaccharide with DP 2-7 can be prepared.

Inventors

• WANG GONGMING
• JING YUEXIN
• JIAO CHUNNA
• CHEN JIANQIANG
• XU YINGJIANG
• QIN HUAWEI
• LIU FANG
• ZHAO YUNPING
• HUANG HUI

Assignees

• 山东省海洋资源与环境研究院(山东省海洋环境监测中心、山东省水产品质量检验中心)

Dates

Publication Date

20260505

Application Date

20260401

Claims (2)

1. 1. The marine bacteria JHS7 is characterized in that Latin name of the marine bacteria JHS7 is Vibrio alginolyticus JHS, the marine bacteria JHS7 is preserved in China general microbiological culture Collection center (China Committee for culture Collection of microorganisms), the preservation date is 2025, 06 and 11 days, the preservation number is CGMCC No.34856, and the place of preservation is Beijing, china.
2. 2. The use of the marine bacterium JHS7 of claim 1 for degrading oyster polysaccharides.

Description

Marine bacterium JHS7 and application thereof Technical Field The invention relates to a bacterium and application thereof, in particular to marine bacterium JHS7 and application thereof in degradation of oyster polysaccharide, and belongs to the technical field of microorganisms. Background Polysaccharides are biological macromolecules composed of more than 10 sugar units through glycosidic bonds. The oyster polysaccharide mainly comprises glucose, the content of which is about 95 percent, is D-pyrane glucan which takes 4-alpha-D-glucose- (1-as a main chain and contains 3, 4-beta-D-glucose- (1-) branched chains, and the relative molecular mass of the D-pyrane glucan is near 1300kDa. Oyster polysaccharide has excellent antiviral, antioxidant, antitumor, hypoglycemic and other bioactivity. The invention patent of oyster polysaccharide preparation method (application number: CN 201610603766.8) discloses a method for preparing oyster polysaccharide without ethanol precipitation, which is safe and reliable to operate and low in cost, the method is easy for industrial production of oyster polysaccharide, and the purity of the prepared oyster polysaccharide is more than or equal to 96%. The biological activity of the saccharides is closely related to the molecular mass and spatial configuration thereof, and researches show that the oligosaccharides have better water solubility than high molecular mass polysaccharides due to shorter chain length and smaller molecular weight, and are easier to interact with proteins. Currently, the reported methods for preparing oligosaccharides from polysaccharides are mainly acid hydrolysis, alkaline hydrolysis and enzymatic hydrolysis. However, acid hydrolysis and alkaline hydrolysis use acid and alkali respectively, so that acid-alkali wastewater to be treated is produced, and glycosidase is required to be used in the enzymolysis method, which is expensive. The biodegradation method has mild reaction conditions and strong specificity, but the related research on preparing low molecular weight oyster oligosaccharides by degrading oyster polysaccharides through specific microorganisms is almost blank, so that the screening of strains capable of efficiently and directionally degrading oyster polysaccharides is needed, the blank of microbial resources of degrading oyster polysaccharides by marine microorganisms is filled, a foundation is laid for developing an industrial production process of high-activity oyster oligosaccharides, and the method has important application value for the development of marine medicaments, functional foods and cosmetic industries. The patent application oyster oligosaccharide beverage (application number 201710507904.7) discloses an oyster oligosaccharide beverage which has low cost and good blood sugar reducing effect and can effectively enhance the sexual function of adult males. The adopted fermentation strain is formed by mixing distiller's yeast, lactobacillus and acetic acid bacteria, but the specific degradation effect of the used strain on oyster polysaccharide is unknown, other reports are not seen, and whether the fermentation substrate is a mixture prepared from 20 substances such as apples or the like is high-efficiency directional degradation of oyster polysaccharide by a certain strain is not clear. Disclosure of Invention In order to solve the defects of the prior art, the invention aims to provide a strain capable of directionally degrading oyster polysaccharide with obvious degradation efficiency. In order to achieve the above object, the present invention adopts the following technical scheme: Vibrio alginolyticus JHS7, latin name Vibrio alginolyticus JHS, is preserved in China general microbiological culture Collection center (China Committee for culture Collection of microorganisms), and has a preservation date of 2025, 06, and 11 days, and a preservation number of CGMCC No.34856, and a preservation unit place of Beijing, china. The application of the marine bacteria JHS7 in degrading oyster polysaccharide. The invention has the advantages that the marine bacteria JHS7 provided by the invention is used for separating and screening natural seawater environment of the sea area of the national smoke table golden beach, the strain can efficiently and directionally degrade oyster polysaccharide, and the relative molecular mass distribution of degradation products is relatively concentrated. Under the conditions that the growth temperature is 30 ℃, the salinity is 3%, the pH value is 8.0 and the inoculation amount is 3%, the degradation rate of the strain on oyster polysaccharide reaches 58.06%, and the relative molecular mass distribution range of degradation products is within 1100Da, so that the oyster oligosaccharide with DP 2-7 can be prepared. Drawings FIG. 1 is a colony morphology of strain JHS 7; FIG. 2 is a diagram of the cell shape of strain JHS 7; FIG. 3 is a phylogenetic tree of strain JHS 7; FIG. 4 is a graph showing the resu