CN-121975702-A - Strain for repairing cadmium-lead-zinc composite pollution and application thereof

Abstract

The invention belongs to the technical field of biological repair of heavy metal pollution, and relates to a strain for repairing cadmium-lead-zinc combined pollution and application thereof, wherein the strain is named as Burkholderia polytricha (Burkholderia multivorans) ZJA3 and has a preservation number of GDMCC NO:67330. The strain can effectively remove Cd, pb and Zn in polluted water, is expected to be used for the treatment and repair of various heavy metal polluted soil, has the growth promoting characteristics of phosphate and potassium dissolving, and provides a very promising solution for green and heavy metal in-situ treatment when the concentration of Cd/Pb/Zn is 10 mu M/50 mu M/100 mu M, and the strain height and biomass of the strain are respectively 1.14 times and 2.71 times that of Burkholderia polytricha ZJA 3.

Inventors

• ZHU ZHIQIANG
• LIU FAN
• YANG YI
• ZOU NAN
• Bi Yichen
• LUO SHANG
• LI LONGSHENG
• ZHENG LICHAO
• WANG QIANQIAN

Assignees

• 海南大学

Dates

Publication Date

20260505

Application Date

20260401

Claims (4)

1. 1. The strain for repairing the cadmium-lead-zinc composite pollution is characterized by being named as Burkholderia polytricha (Burkholderia multivorans) ZJA3, the preservation number is GDMCC NO:67330, and the Burkholderia polytricha ZJA3 has the growth promoting characteristics of removing the cadmium-lead-zinc composite pollution and dissolving phosphorus and potassium.
2. 2. The use of the strain of claim 1 for repairing cadmium lead zinc complex pollution.
3. 3. The use according to claim 2, characterized in that it comprises increasing the plant height and biomass of plants inoculated with said burkholderia polytricha ZJA3 in a contaminated water body.
4. 4. A biological agent for repairing cadmium-lead-zinc composite pollution, which is characterized by comprising the strain of claim 1.

Description

Strain for repairing cadmium-lead-zinc composite pollution and application thereof Technical Field The invention belongs to the technical field of bioremediation of heavy metal pollution, and relates to a strain for remedying cadmium-lead-zinc combined pollution and application thereof. Background Due to the rapid urban development and the widespread use of farm manure, sewage sludge and chemical fertilizers, excessive heavy metals can accumulate in the soil. These heavy metals can continue to enter the ecosystem by natural release and artificial activity and are extremely easy to enrich by the food chain due to their high fluidity and bioavailability. At present, the repairing method for heavy metal contaminated soil mainly comprises physical, chemical and biological repairing technologies. Compared with physical and chemical restoration means, biological restoration technology is receiving attention because of its relatively low cost and better environmental compatibility. Most of the super-enriched plants have the limitations of short plants, low biomass, slow growth, long repair period and the like, so that the repair efficiency can be improved by applying strengthening measures to the super-enriched plants, and the microorganism combined with the plant strengthening repair measures is a relatively effective bioremediation approach. In the face of the special and treatment problems of heavy metal pollution of soil in high geological background areas, the microorganism repairing technology shows irreplaceable huge potential. However, resistant microorganisms obtained by screening often have a single function or are only resistant to heavy metals but lack synergistic effects for promoting plant growth, and it is difficult to achieve soil restoration while improving land productivity. Disclosure of Invention Aiming at the technical problems, the invention aims to provide a strain for repairing cadmium-lead-zinc composite pollution and application thereof, and the strain can be used for treating and repairing various heavy metal polluted water bodies or soil, has phosphate and potassium dissolving and growth promoting characteristics, and provides a very promising solution for green and heavy metal in-situ treatment. The technical scheme adopted for realizing the technical purpose is as follows: The invention provides a strain for repairing cadmium-lead-zinc composite pollution, which is named as Burkholderia spinosa (Burkholderia multivorans) ZJA3 and has the preservation number of GDMCC NO:67330. Preferably, the 16S rDNA sequence of the Burkholderia polysaccharea ZJA3 is shown as SEQ ID NO. 1. Preferably, the maximum tolerance concentration of Burkholderia polycephala ZJA3 to heavy metal cadmium (Cd), lead (Pb) and zinc (Zn) is greater than 80 mg/L, 600 mg/L and 1200 mg/L, respectively. Preferably, the Burkholderia polycephala ZJA3 has the growth promoting property of removing cadmium, lead and zinc combined pollution and dissolving phosphorus and potassium. The invention also provides application of the strain in repairing cadmium-lead-zinc composite pollution. Preferably, the application comprises removing cadmium lead zinc in a contaminated water or soil and promoting growth by phosphate and potassium dissolution. More preferably, the use comprises increasing the plant height and biomass of plants inoculated with said burkholderia polytricha ZJA3 in a contaminated water body. The invention also provides a biological microbial agent for repairing cadmium, lead and zinc composite pollution, which comprises the strain. The invention has the beneficial effects that: The invention separates and screens out a Burkholderia polytricha (Burkholderia multivorans) ZJA3 which can simultaneously have high tolerance to cadmium, lead and zinc from the soil of a Yunnan mining area, and the highest tolerance concentration of the strain to heavy metal cadmium (Cd), lead (Pb) and zinc (Zn) is respectively more than 80 mg/L, 600 mg/L and 1200 mg/L; the strain can effectively remove Cd, pb and Zn in polluted water, the removal rates reach 27.84%, 98.38% and 4.63% respectively, the strain is expected to be used for the treatment and repair of various heavy metal polluted soil, meanwhile, the strain also has the growth promoting characteristics of phosphate and potassium dissolving, when the concentration of Cd/Pb/Zn is 10 mu M/50 mu M/100 mu M, the king grass plant is inoculated with Burkholderia polytricha ZJA3, the plant height and biomass are 1.14 times and 2.71 times of that of the strain which is not subjected to bacterial treatment respectively, and the strain has obvious growth promoting effect, so that the strain provides a very promising solution for green and heavy metal in-situ treatment. Drawings FIG. 1 is a phylogenetic tree of Burkholderia polycephala ZJA3 of the present invention. FIG. 2 shows the growth state of Burkholderia polycephala ZJA3 of the present invention on a solid medium. FIG. 3 shows the effect