CN-121975703-A - Bacillus amyloliquefaciens and application thereof

Abstract

The invention belongs to the technical field of agricultural microorganisms, and relates to bacillus amyloliquefaciens and application thereof. Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) D4 has a preservation number of GDMCC No:66921, a preservation time of 2025, 9 and 5 days, and a preservation address of Guangzhou municipal martyr, no. 59 building 5 building Guangdong national institute of sciences of China. The invention provides a novel bacillus amyloliquefaciens, which has stronger antagonism to potato black shank and sweet potato stem rot and is favorable for being applied to the aspect of preventing and treating related diseases. The strain D4 is convenient to produce and prepare, low in cost and simple in flow, and is beneficial to large-scale manufacture and use. The strain D4 and the bacterial suspension and the fermentation liquor thereof can be used as active ingredients of the control medicament to be applied to actual production on a large scale.

Inventors

• QIU HAIPING
• LU YU
• ZHU XUHUI
• LI CHENHAO
• YAN TIE
• HAO ZHONGNA
• CHAI RONGYAO
• ZHANG ZHEN
• WANG JING
• CAI YINGYING
• WANG JIAOYU
• WANG YANLI

Assignees

• 浙江省农业科学院
• 浙江省植保检疫与农药管理总站

Dates

Publication Date

20260505

Application Date

20260407

Claims (10)

1. 1. Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) D4 with deposit number GDMCC No:66921.
2. 2. The bacillus amyloliquefaciens of claim 1, wherein the sequence of the strain 16S is shown in SEQ ID No. 1.
3. 3. A culture, fermentation broth or bacterial suspension comprising a Bacillus amyloliquefaciens of claim 1.
4. 4. Use of a bacillus amyloliquefaciens according to claim 1 or a culture, fermentation broth or bacterial suspension according to claim 3 for inhibiting pathogenic bacteria.
5. 5. The use according to claim 4, wherein the pathogenic bacteria comprise potato early blight bacteria, potato anthracnose bacteria, potato gray mold bacteria, watermelon vine blight bacteria, rice bakanae bacteria and/or rice blast bacteria.
6. 6. Use of a bacillus amyloliquefaciens according to claim 1 or a culture solution, fermentation broth or bacterial suspension according to claim 3 for the preparation of a plant biocontrol agent.
7. 7. Use of a bacillus amyloliquefaciens according to claim 1 or a culture solution, fermentation broth or bacterial suspension according to claim 3 for controlling plant diseases.
8. 8. The use of claim 7, wherein the plant disease comprises potato scab, potato black shank, and/or sweet potato stem rot.
9. 9. An agent for controlling potato scab, potato black shank and sweet potato stem rot, characterized in that the active ingredient of the agent comprises the bacillus amyloliquefaciens of claim 1 or the culture solution, fermentation solution or bacterial suspension of the bacillus amyloliquefaciens of claim 3.
10. 10. A method for controlling potato scab, potato black shank and sweet potato stem rot, characterized in that the agent of claim 9 is sprayed in the seedling stage and the expansion stage of plants, respectively.

Description

Bacillus amyloliquefaciens and application thereof Technical Field The invention belongs to the technical field of agricultural microorganisms, and relates to bacillus amyloliquefaciens and application thereof. Background Sweet potato (Dioscorea esculenta) belongs to Dioscoreaceae, and is a perennial winding vine plant. Sweet potato is rich in starch, dietary fiber, vitamin A, vitamin C and minerals. The starch content is 20% -30%, so that the feeling of satiety can be increased. Beta-carotene in sweet potato has antioxidant effect, and is helpful for delaying aging, and vitamin C can enhance immunity. In addition, dietary fiber of sweet potato helps to promote intestinal health, and potassium helps to maintain heart function and regulate blood pressure. The sweet potato stem rot is a bacterial disease caused by the Samson et al DICKEYA DADANTII of Dekkera dadanti, belongs to a new disease in China, and has the advantages of rapid spread, difficult control and serious influence on the quality and yield of sweet potatoes, and becomes one of main diseases in south sweet potato production areas in China. At present, a method for simultaneously using disease-free seedlings and chemical control is mainly adopted for controlling sweet potato stem rot, but the method has high technical threshold and cost, is difficult to ensure disease-free in reality, has risks of reinfection and incubation period, and has the defects of single variety of disease-free plants, unstable supply and other factors which influence the actual large-scale implementation of the method. Potato (Solanum tuberosum l.) belongs to the genus solanum of the family solanaceae, being the fourth most important food crop worldwide, next to wheat, rice and maize. Potatoes play a vital role in global food safety due to the characteristics of strong adaptability, high yield and comprehensive nutrition. The potato black shank is a bacterial disease caused by Erwinia carotovora, and mainly affects the stem base and tubers of potatoes, and the prevention measures comprise selecting disease-resistant varieties and strengthening cultivation management. The black shank of the potato commonly occurs in northeast, north China, northwest China and other areas, and the incidence rate can reach 40-50%. The disease not only affects potatoes, but also infects other plants including Solanaceae, cucurbitaceae, and Leguminosae. The germs are mainly transmitted through the seed potato with diseases and soil, and root systems and tubers of the disease plants can become infection sources in the next season after harvesting. The current effective measures for preventing and treating potato black shank comprise selecting disease-resistant varieties (epidemic resistant No. 1, victory No. 1, and the like), and establishing a disease-free seed field or chemical prevention and treatment (benziothiazolinone). However, long-term use of chemical agents such as benziothiazolinone and the like can easily cause drug resistance of sweet potatoes, and excessive use can influence soil microbial communities and can enter the environment through infiltration or residues. Potato scab is a common bacterial disease caused by multiple pathogenic bacteria of the genus Streptomyces (Streptomyces spp.), one of the major diseases affecting the commercial value of potatoes. The disease mainly infects tubers, and although the influence on the yield is relatively small, the appearance quality of the tubers is seriously damaged, and the disease is recognized as one of four main diseases in potato production. Bacteria are decomposed in soil or overwintered on seed potatoes with diseases, light brown small spots are formed on tuber surfaces through penetration of tuber pores or wounds, and then the small spots are gradually expanded into round or irregular concave lesions, the lesions are formed by wood bolting, the surface is rough, and typical scabs are formed. The disease is particularly serious in warm dry, neutral or slightly alkaline sandy loam, and the disease is aggravated by continuous cropping land plots. The current effective measures for preventing and treating potato scab mainly comprise planting disease-resistant varieties (such as Hisen No. 5 and Ji Zhangshu, etc.), carrying out rotation on non-host crops for more than 5 years, selecting disease-free potato seeds, carrying out disinfection treatment, regulating the pH value of soil and strengthening cultivation management (such as keeping soil moist in potato growing period). However, the existing method lacks of high-resistance varieties, has long disease-resistant breeding period and high cost, has limited chemical control effect and is easy to pollute the environment, and biological control has potential but is not mature in application, so that the prevention and control of the disease still mainly depend on comprehensive agricultural management measures. Disclosure of Invention Aiming at the problems existing in the prior art, the