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CN-121975705-A - Low-temperature aerobic denitrifying Pseudomonas sp.D2-1 a and application thereof

CN121975705ACN 121975705 ACN121975705 ACN 121975705ACN-121975705-A

Abstract

The application relates to a low-temperature aerobic denitrifying Pseudomonas sp.D2-1a and application thereof, belonging to the technical field of microorganisms. The strain expands a resource library of aerobic denitrifying bacteria in a polar region low-temperature environment, and provides theoretical support for revealing an aerobic denitrification mechanism under a cold condition and optimizing a sewage treatment process in the cold region.

Inventors

  • LI HUIRONG
  • MA JIAYI
  • DING HAITAO
  • YU YONG
  • SI HUIMIN

Assignees

  • 中国极地研究中心(中国极地研究所)

Dates

Publication Date
20260505
Application Date
20260409

Claims (7)

  1. 1. The Pseudomonas D2-1a is characterized in that the Pseudomonas D2-1a is classified and named as Pseudomonas sp, and the preservation number is CGMCC No.37028 and is preserved in the China general microbiological culture Collection center (China general microbiological culture Collection center) at 12-11 of 2025.
  2. 2. A formulation comprising pseudomonas D2-1a according to claim 1, or a spore suspension thereof, or a fermentation broth thereof, or a lyophilized powder thereof.
  3. 3. A sewage treatment agent, which comprises pseudomonas D2-1a, or spore suspension thereof, or fermentation broth thereof, or freeze-dried powder thereof according to claim 1.
  4. 4. Use of pseudomonas D2-1a according to claim 1 for aerobic denitrification.
  5. 5. Use of pseudomonas D2-1a according to claim 1 for the removal of nitrate nitrogen.
  6. 6. Use of pseudomonas D2-1a according to claim 1 for wastewater treatment.
  7. 7. The use of pseudomonas D2-1a according to claim 1 for sewage treatment in cold areas.

Description

Low-temperature aerobic denitrifying Pseudomonas sp.D2-1 a and application thereof Technical Field The application belongs to the technical field of microorganisms, and particularly relates to a low-temperature aerobic denitrifying bacterium Pseudomonas sp.D2-1 a and application thereof. Background Nitrogen is an important nutrient element in water, but along with the aggravation of human activities, a large amount of nitrogen-containing wastewater is continuously discharged into the environment, so that the ecological environment problems of eutrophication of the water, red tide frequency, hypoxia and the like are increasingly prominent. Therefore, the efficient removal of nitrogen pollutants in the emissions is of great significance in maintaining the stability of the water ecosystem and guaranteeing the safety of the water environment. Compared with physical and chemical methods, biological denitrification is considered as a main way of sewage denitrification due to the advantages of high efficiency, economy, environmental friendliness and the like. Traditional biological denitrification processes generally rely on sequential nitrification and denitrification processes, but due to the difference in oxygen and carbon source requirements, staged operation is often required in practical applications, thereby increasing process complexity and operation cost. Since the first separation of Thiosphaera pantotropha (now designated Paracoccus denitrificans) from activated sludge in the 80 s of the 20 th century, which is capable of completing nitrification and denitrification simultaneously under aerobic conditions, aerobic denitrification (HN-AD) has attracted increasing attention. Nevertheless, the current research has focused on medium temperature conditions, and isolated strains are mainly derived from activated sludge, soil and fresh water environments. Under the low temperature condition, the efficiency of the conventional nitrification-denitrification process is obviously reduced, and the denitrification effect of sewage treatment plants in cold areas is severely restricted. At present, although few low temperature resistant Aerobic Denitrifying Bacteria (ADB) are reported, most reports focus on the removal efficiency of single strains under specific conditions, and the intensive mechanism researches about molecular mechanisms, enzyme activity regulation, gene expression cold adaptation mechanisms and the like of the single strains are still in shortage. Therefore, the screening of the bacterial strain capable of efficiently denitrifying under the low-temperature condition has important significance for breaking through the bottleneck of sewage treatment in cold areas. The polar environment is under low temperature and multiple environmental stress for a long time, and contains rich microorganisms with special metabolic characteristics. These microorganisms may not only exhibit excellent nitrogen removal ability, but may also carry unique low temperature adaptation genes. Therefore, the screening of the cold-fit aerobic denitrifying bacteria under the condition of extremely low temperature throughout the year is quite possible. Disclosure of Invention The application provides a low-temperature efficient aerobic denitrifying strain derived from a Antarctic typical environment sample and application thereof, aiming at solving the technical problems that the denitrification efficiency of the existing aerobic denitrifying strain is reduced under the low-temperature condition, nitrite is easy to accumulate and the like. And 449 strains with aerobic denitrification capability are obtained by adopting the co-separation of an improved denitrification culture medium, and 7 representative strains with highest denitrification efficiency under the condition of 15 ℃ are screened out by 48h denitrification performance measurement. The removal rate of nitrate in 48h of the 7 strains is over 99.8 percent, the accumulation amount of nitrite is lower than 1.5 mu mol/L, and the 7 strains show excellent denitrification capability. The 7 strains are further subjected to whole genome sequencing and functional annotation, and the result shows that 4 strains, namely D2-1a, W2-12b, H2-1 and ND2-8, have complete denitrification paths, all of the strains carry various low-temperature adaptation related genes, and a molecular basis is provided for maintaining high-efficiency metabolism in a low-temperature environment. Genomic comparative analysis shows that the D2-1a strain and the most similar mode strain ANI are less than 95%, and DDH is less than 70%, thus having potential new species characteristics. The nitrogen balance analysis result shows that the denitrification process of the strain is mainly completed through the assimilation and the aerobic denitrification. The metabolic path of the strain to nitrate nitrogen is NO 3-→ NO2-→ NO → N2O → N2 by combining genome annotation, enzyme activity measurement and nitrogen balance an