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CN-121975713-A - Culture medium containing microorganism growth promoter and novel carbon source and preparation method thereof

CN121975713ACN 121975713 ACN121975713 ACN 121975713ACN-121975713-A

Abstract

The invention discloses a culture medium containing a microorganism growth promoter and a novel carbon source and a preparation method thereof, belonging to the technical field of microorganism fermentation engineering. The culture medium comprises, by mass, 15-30 parts of a novel carbon source, 5-10 parts of a nitrogen source, 2-5 parts of an inorganic salt mixture, 0.1-2 parts of a microorganism growth promoter, 1-3 parts of a buffering agent, 0.1-3 parts of a multifunctional composite factor and purified water. Wherein the microorganism growth promoter is an amide compound with a specific structure shown in a formula 1. According to the invention, through the synergistic effect of the growth promoter with a specific structure and a novel carbon source (such as trehalose, soluble starch and the like), the microbial cell membrane permeability is improved, and the activity of the carbon source catabolic enzyme is activated, so that the problems of low utilization rate of the novel carbon source when being singly used and pH dip and product inhibition caused by too fast metabolism of the traditional carbon source are effectively solved.

Inventors

  • JIA YAFEI
  • ZHU WEIYE
  • Zhang Gaga

Assignees

  • 禹沐源生物科技(重庆)有限公司

Dates

Publication Date
20260505
Application Date
20260306

Claims (10)

  1. 1. A culture medium containing a microorganism growth promoter and a novel carbon source is characterized by comprising the following raw materials, by mass, 15-30 parts of the novel carbon source, 5-10 parts of a nitrogen source, 2-5 parts of an inorganic salt mixture, 0.1-2 parts of the microorganism growth promoter, 1-3 parts of a buffering agent, 0.1-3 parts of a multifunctional composite factor and 100 parts of purified water; The structural formula of the microbial growth promoter is a compound shown in a formula 1; 1 (1) ; R 1 in the formula 1 is selected from hydrogen, bromine, chlorine and methyl.
  2. 2. The medium of claim 1, wherein the novel carbon source is at least one of trehalose, soluble starch, and glycerol.
  3. 3. The medium of claim 1, wherein the nitrogen source is at least one of yeast extract, tryptone, and ammonium sulfate.
  4. 4. The medium containing a microbial growth promoter and a novel carbon source according to claim 1, wherein the inorganic salt mixture comprises, by mass, 0.5-1 part of KH 2 PO 4 , 0.5-1 part of K 2 HPO 4 , 0.2-0.5 part of MgSO 4 ·7H 2 O and 0.1-0.3 part of NaCl.
  5. 5. The medium of claim 1, wherein the buffer is a sodium citrate-citric acid buffer system.
  6. 6. The medium containing a microbial growth promoter and a novel carbon source according to claim 1, wherein the multifunctional complex factor is at least one of spermidine and spermine.
  7. 7. The medium containing a microbial growth promoter and a novel carbon source according to claim 1, wherein the microbial growth promoter is any one of the compounds represented by the following structures: 。
  8. 8. a method for preparing a medium containing a microbial growth promoter, a novel carbon source according to any one of claims 1 to 7, comprising the steps of: S1, all components except a microorganism growth promoter and a multifunctional composite factor are dissolved in 3/5-4/5 parts by mass of purified water, heated and stirred uniformly to form a basic solution; S2, adjusting the pH value of the basic solution to 6.8-7.2 by using 1mol/L sodium hydroxide solution or 1mol/L hydrochloric acid solution; s3, adding the microorganism growth promoter and the multifunctional composite factor into the basic solution, and stirring and uniformly mixing; S4, using 1/5-2/5 of purified water to fix the volume to the final volume, uniformly mixing, and performing high-pressure steam sterilization treatment to obtain a culture medium containing a microorganism growth promoter and a novel carbon source; The conditions of autoclaving are 121 ℃ for 15-20 minutes.
  9. 9. The method for preparing a medium containing a microbial growth promoter and a novel carbon source according to claim 8, wherein the heating temperature in step S2 is 60 to 80 ℃ and the stirring speed is 150 to 250rpm.
  10. 10. Use of a medium according to any one of claims 1-7, comprising a microbial growth promoter, a novel carbon source, for the cultivation of bacillus subtilis, lactococcus lactis.

Description

Culture medium containing microorganism growth promoter and novel carbon source and preparation method thereof Technical Field The invention relates to the technical field of microbial fermentation engineering, in particular to a culture medium containing a microbial growth promoter and a novel carbon source and a preparation method thereof. Background The microbial culture technology is core support in the fields of biological fermentation, environmental management, food industry, biological medicine and the like, the culture medium is used as a nutrition carrier for the growth and propagation of microorganisms, and the component design directly determines the growth rate, biomass and metabolic activity of the microorganisms. The carbon source is used as a core substrate for microbial energy metabolism, and the traditional culture medium is usually glucose, sucrose and other monosaccharides or disaccharides, so that the traditional culture medium is easy to be utilized by microorganisms, but the problems of sudden pH drop, obvious product inhibition effect, high cost and the like of the culture medium caused by too fast metabolic rate exist, and the requirements of high-density culture and industrialized mass production are difficult to meet. The novel carbon source such as trehalose, soluble starch, glycerol and the like gradually becomes a substitute choice of the traditional carbon source by virtue of the advantages of strong stability, mild metabolism, low cost, difficult pollution initiation and the like, but the decomposition and absorption efficiency of the carbon source in microbial cells is lower, the nutrition potential of the carbon source is difficult to fully develop when the carbon source is singly used, and the application and popularization of the carbon source in a high-efficiency culture medium are limited. The microbial growth promoter is used as a key functional component of the culture medium, and can improve the utilization rate of the microorganism to nutrient substances by regulating the metabolic pathway of the microorganism, improving the permeability of cell membranes, activating the activity of enzymes and other mechanisms. However, most of the existing growth promoters are natural extracts (such as plant extracts and microbial metabolites), and have the defects of complex ingredients, unstable activity, fuzzy action mechanism and the like, while some of the chemical synthesis promoters have the problems of high dosage requirement, poor biocompatibility, weak pertinence and the like, and lack of special preparations with synergistic effect with novel carbon sources, so that the existing culture medium still has a larger improvement space in microbial growth promotion effect. In addition, the existing culture medium often has the problems of unreasonable component collocation, weak synergistic effect of carbon source and growth promoter, and the like, so that the dilemma of long growth cycle, low biomass, insufficient metabolite yield, and the like is generally faced in the culture process of bacillus subtilis and lactococcus lactis, and the production efficiency and industrialization process in the related fields are severely restricted. Therefore, the development of the microbial growth promoter which is adaptive to a novel carbon source, clear in structure, high in efficiency and low in dosage, and the construction of a culture medium system which is cooperatively optimized by the novel carbon source and the microbial growth promoter solve the defect of nutrition supply of the traditional culture medium, and become a key problem to be broken through in the technical field of microbial culture at present. Disclosure of Invention Aiming at the problems of defects of traditional carbon sources, unstable activity of microorganism growth promoters, weak synergistic effect of the carbon sources and the promoters and the like in the prior art, the invention provides a culture medium of the microorganism growth promoters and novel carbon sources, which has reasonable component collocation, high efficiency and low dosage, can improve the utilization rate of microorganisms to nutrition, shortens the growth period, improves the biomass, adapts to the culture requirements of bacillus subtilis and lactococcus lactis, and meets the requirements of industrial production, and a preparation method thereof. In order to achieve the above purpose, the invention adopts the following technical scheme: A culture medium containing a microorganism growth promoter and a novel carbon source comprises the following raw materials, by mass, 15-30 parts of the novel carbon source, 5-10 parts of a nitrogen source, 2-5 parts of an inorganic salt mixture, 0.1-2 parts of the microorganism growth promoter, 1-3 parts of a buffering agent, 0.1-3 parts of a multifunctional composite factor and 100 parts of purified water. The structural formula of the microbial growth promoter is a compound shown in a formula 1; 1