CN-121975729-A - Application of extract in proliferation and differentiation of mesenchymal stem cells

CN121975729ACN 121975729 ACN121975729 ACN 121975729ACN-121975729-A

Abstract

The invention discloses application of an extract in mesenchymal stem cell proliferation and differentiation, and relates to the technical field of biological medicine. The invention specifically discloses extracts of at least one of perilla, black sesame, lophatherum gracile, lotus leaf, mulberry leaf, houttuynia cordata, angelica dahurica, dark plum, fresh cogongrass rhizome, coix seed, chicory, fructus alpiniae oxyphyllae, astragalus mongholicus, dandelion, chrysanthemum, dandelion, citron, gardenia, fresh reed rhizome, orange peel, radish seed, peppermint, perilla seed, fermented soybean, hemp seed, sealwort, viper, semen brassicae, red bean, red date, hawthorn, bunge cherry seed, raspberry, torreya, ginseng, kudzuvine root, spina date seed, orange peel, black pepper, platycodon grandiflorum, phyllanthus, longan, hovenia dulcis thunb, wrinkled gianthyssop, green oliv fruit, momordica seed, cassia seed, fingered citron, eucommia ulmoides, galangal, medlar and white hyacinth bean.

Inventors

YANG YUAN
LIU YUN
YU SIJIE
ZHENG CHUNBING

Assignees

源品细胞生物科技集团有限公司

Dates

Publication Date: 20260505
Application Date: 20260106

Claims (10)

1. Use of an extract according to any one of A1) to A4), characterized in that the extract comprises an extract of at least one of perilla, black sesame, lophatherum gracile, lotus leaf, mulberry leaf, houttuynia cordata, angelica dahurica, dark plum, fresh cogongrass rhizome, coix seed, chicory, alpinia oxyphylla, astragalus mongholicus, dandelion, chrysanthemum, dandelion, citron, gardenia, fresh reed rhizome, orange peel, radish seed, peppermint, perilla seed, fermented soybean, hemp seed, sealwort, viper, yellow mustard seed, red bean, red date, hawthorn, bunge cherry seed, raspberry, torreya seed, ginseng, kudzuvine root, wild jujube, orange peel, black pepper, platycodon grandiflorum, emblica officinalis, longan, hovenia, wrinkled gianthyssop, fructus momordicae, cassia seed, fingered citron, eucommia ulmoides, galangal, medlar and white hyacinth bean; a1 Regulating mesenchymal stem cell proliferation; a2 Preparing a product for regulating and controlling mesenchymal stem cell proliferation; a3 Maintaining the multipotent differentiation potential of mesenchymal stem cells; a4 A product that maintains the multipotent differentiation potential of mesenchymal stem cells.
2. The use according to claim 1, wherein the extract is an aqueous extract.
3. The use according to claim 1, wherein said modulating mesenchymal stem cell proliferation is promoting mesenchymal stem cell proliferation; the action concentration of the perilla extract is 0.1 mg/mL-8 mg/mL; and/or the action concentration of the extract of the black sesame is 0.3 mg/mL-10 mg/mL; and/or the extract of Lophatherum gracile has an action concentration of 0.1 mg/mL-9 mg/mL, and/or the extract of Lophatherum gracile has an action concentration of 0.1 mg/mL-7 mg/mL, and/or the extract of Lophatherum gracile has an action concentration of 0.1 mg/mL-6 mg/mL, and/or the extract of Lophatherum gracile has an action concentration of 0.1 mg/mL-4.5 mg/mL, and/or the extract of Lophatherum gracile has an action concentration of 0.1 mg/mL-15 mg/mL, and/or the extract of Lophatherum gracile has an action concentration of 0.1 mg/mL-10 mg/mL, and/or the extract of Curculigo root has an action concentration of 0.1 mg/mL-9/mL, and/or the extract of Job's tears seed has an action concentration of 0.1.3656/mL-4.5 mg/mL, and/or the extract of Lophatherum gracile has an action concentration of 0.1-mg/mL, and/or the extract of 3.3938/mL, and/or the extract of 3.3938/or 3.2/mL, the extract of the dandelion fruit has an action concentration of not 0.1 mg/mL-10 mg/mL, and/or the extract of the chrysanthemum flower has an action concentration of 0.1 mg/mL-7 mg/mL, and/or the extract of the citrus fruit has an action concentration of 0.1 mg/mL-3 mg/mL, and/or the extract of the dandelion fruit has an action concentration of 0.1 mg/mL-6 mg/mL, and/or the extract of the gardenia fruit has an action concentration of 0.1 mg/mL-3 mg/mL, and/or the extract of the fresh reed rhizome has an action concentration of 0.1 mg/mL-3 mg/mL, and/or the extract of the citrus fruit has an action concentration of 1 mg/mL-6/mL, and/or the extract of the mint fruit has an action concentration of 0.3932/mL-6/mL, and/or the extract of the mint fruit has an action of 0.3932/mL-3/mL, and/or the extract of 3932.3932/or the extract of the mint fruit has an action of 0.3932/mL-3/mL, and/or the extract of the mint fruit has an action of 3932.3932/mL-mg/mL, and/or the extract of the mint fruit has an action of mg/3/mL-mg/mL, the red bean extract has an action concentration of 0.1 mg/mL-8 mg/mL, the red jujube extract has an action concentration of 0.1 mg/mL-10 mg/mL, the haw extract has an action concentration of 0.1 mg/mL-10 mg/mL, the bunge cherry seed extract has an action concentration of 0.1 mg/mL-1 mg/mL, the raspberry extract has an action concentration of 0.1 mg/mL-6 mg/mL, the Chinese walnut extract has an action concentration of 0.1 mg/mL-6 mg/mL, and/or the Chinese walnut extract has an action concentration of 0.1 mg/mL-10 mg/mL, the kudzu root extract has an action concentration of 0.1 mg/mL-6/mL, and/or the orange extract has an action concentration of 0.3932/mL-3.3932/mL, the orange extract has an action concentration of 0.1 mg/mL-3.3932/mL, the orange extract has an action of 3932.3932/mL-3.3932/mL, and/or the orange extract has an action of 3932.3932/mL-3.1.3932/mL, the action concentration of the extract of platycodon grandiflorum is 0.1 mg/mL-3 mg/mL, the action concentration of the extract of Chinese olive is 0.1 mg/mL-10 mg/mL, the action concentration of the extract of momordica grosvenori is 0.1 mg/mL-10 mg/mL, the action concentration of the extract of cassia seed is 0.1 mg/mL-10 mg/mL, the action concentration of the extract of bergamot is 0.1 mg/mL-3 mg/mL, the action concentration of the extract of eucommia ulmoides is 0.1 mg/mL-3 mg/mL, the action concentration of the extract of galangal is 0.1 mg/mL-1 mg/mL, and/or the action concentration of the extract of medlar is 0.1 mg/mL-10 mg/mL, and/or the action concentration of white beans is 0.1-5428/mL.
4. The use according to claim 1, wherein the extract of A3) or A4) comprises an extract of at least one of perilla, lophatherum gracile, lotus leaf, black sesame and mulberry leaf.
5. The use according to claim 4, wherein the action concentration of the extract of the perilla is 0.1 mg/mL to 8mg/mL, and/or the action concentration of the extract of the lophatherum gracile is 0.1 mg/mL to 9 mg/mL, and/or the action concentration of the extract of the lotus leaf is 0.1 mg/mL to 7 mg/mL, and/or the action concentration of the extract of the black sesame is 0.3 mg/mL to 10mg/mL, and/or the action concentration of the extract of the mulberry leaf is 0.1 mg/mL to 6 mg/mL.
6. The use according to claim 1, characterized in that the preparation method of the extract comprises the following steps: Preparing at least one of perilla, black sesame, lophatherum gracile, lotus leaf, mulberry leaf, cordate houttuynia, angelica dahurica, dark plum, fresh cogongrass rhizome, coix seed, chicory, fructus alpiniae oxyphyllae, astragalus membranaceus, dandelion fruit, chrysanthemum, dandelion, citron fruit, gardenia, fresh reed rhizome, orange peel, radish seed, peppermint, perilla seed, fermented soybean, hemp seed, rhizoma polygonati, viper, yellow mustard seed, red bean, red date, hawthorn, bunge cherry seed, raspberry, chinese torreya seed, ginseng, kudzuvine root, spina date seed, orange, black pepper, platycodon grandiflorum, phyllanthus emblica, longan, hovenia, wrinkled gianthyssop, chinese olive, fructus momordicae, cassia seed, fingered citron, eucommia bark, galangal, medlar and white hyacinth bean, and extracting with water to obtain the extract.
7. The use according to claim 6, wherein the feed ratio of the mixture is 1 g (5-10) mL.
8. The use according to claim 6, wherein the total time of the aqueous extraction is 2 h-5 h.
9. The use according to claim 1, wherein the product comprises at least one of a reagent, a kit, a medicament.
10. A mesenchymal stem cell culture medium comprising the extract according to any one of claims 1 to 9.

Description

Application of extract in proliferation and differentiation of mesenchymal stem cells Technical Field The invention relates to the technical field of biological medicine, in particular to application of an extract in mesenchymal stem cell proliferation and differentiation. Background Mesenchymal Stem Cells (MSCs) are adult stem cells with self-renewal and multidirectional differentiation potential, and have great application prospects in the fields of regenerative medicine, tissue engineering, immunoregulation, disease treatment and the like. However, studies have shown that their proliferative capacity and differentiation potential decrease significantly with increasing number of passages in vitro. Currently, conventional in vitro culture systems for MSCs rely on Fetal Bovine Serum (FBS) as the main additive component of the culture medium. Although FBS contains abundant growth factors and nutrients and can effectively support cell growth, the FBS has the risks of high biosafety risk, immune rejection, large batch-to-batch difference and the like. Although serum-free media, chemically defined media, and the like have been developed, there are problems in that they are expensive, the proliferation promoting effect and the cell function maintenance ability are inferior to those of FBS, and chemical synthetic small molecules are added, and long-term biosafety thereof has yet to be evaluated. Accordingly, it is desirable to provide a substance capable of promoting proliferation of MSCs and effectively maintaining differentiation performance of MSCs. Disclosure of Invention The present invention aims to solve at least one of the technical problems existing in the prior art. For this purpose, the invention proposes the use of an extract. The invention also provides a culture medium comprising the extract. Use of an extract according to an embodiment of the first aspect of the present invention in any one of A1) to A4), the extract comprising an extract of at least one of perilla, black sesame, lophatherum gracile, lotus leaf, mulberry leaf, houttuynia cordata, angelica dahurica, dark plum, fresh cogongrass rhizome, coix seed, chicory, alpinia oxyphylla, astragalus mongholicus, dandelion, chrysanthemum, dandelion, citron, gardenia, fresh reed rhizome, orange peel, radish seed, peppermint, perilla seed, fermented soybean, hemp seed, rhizoma polygonati, viper, semen brassicae, red bean, red date, hawthorn, bunge cherry seed, raspberry, torreya seed, ginseng, kudzuvine root, spina date seed, orange, black pepper, platycodon grandiflorum, emblic, longan, hovenia, wrinkled gianthyssop, green fruit, momordica, cassia seed, fingered citron, eucommia bark, galangal, medlar, white hyacinth bean; a1 Regulating mesenchymal stem cell proliferation; a2 Preparing a product for regulating and controlling mesenchymal stem cell proliferation; a3 Maintaining the multipotent differentiation potential of mesenchymal stem cells; a4 A product that maintains the multipotent differentiation potential of mesenchymal stem cells. The application according to the embodiment of the invention has at least the following beneficial effects: The extracts of the examples are all derived from the raw materials of homology of medicine and food, have long-term human eating history, have biological safety far higher than animal-derived serum (such as fetal bovine serum) and chemical synthetic drugs, and greatly reduce the risks of virus and mycoplasma pollution and immune rejection in clinical application. The extract of the embodiment can regulate the proliferation condition of mesenchymal stem cells. And the cell morphology of the mesenchymal stem cells after treatment is kept good, the positive rate of the specific surface markers of the stem cells is still kept above 95 percent, and meanwhile, the cells still keep the multipotent differentiation potential of osteogenesis, adipogenesis, chondrogenesis and the like. The extract of the examples can effectively maintain the "stem" and function of the cells while regulating the proliferation of mesenchymal stem cells, rather than inducing malignant transformation or aging thereof. According to some embodiments of the invention, the extract is an aqueous extract. According to some embodiments of the invention, the product comprises at least one of a reagent, a kit, a medicament. According to some embodiments of the invention, the multipotent differentiation potential is the ability to differentiate into osteoblasts, adipogenic cells or chondroblasts upon stimulation with a specific induction medium. According to some embodiments of the invention, the method of preparing the extract comprises the steps of: Preparing at least one of perilla, black sesame, lophatherum gracile, lotus leaf, mulberry leaf, cordate houttuynia, angelica dahurica, dark plum, fresh cogongrass rhizome, coix seed, chicory, fructus alpiniae oxyphyllae, astragalus membranaceus, dandelion fruit, chrysanthemum, dandelion, citron fruit