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CN-121975736-A - Application of aloe polysaccharide in-vitro regulated phagocytic macrophages in promoting wound healing

CN121975736ACN 121975736 ACN121975736 ACN 121975736ACN-121975736-A

Abstract

The invention discloses application of aloe polysaccharide in-vitro regulated phagocytic macrophages in promoting wound healing. In the invention, the aloe polysaccharide pervaporation membrane can be used for preparing phagocytic macrophages, and the aloe polysaccharide pervaporation membrane is added into a culture medium of the macrophages, so that the result shows that the phagocytic capacity of the macrophages is obviously improved, and further experiments show that the phagocytic macrophages are used for wound treatment, so that the inflammation level of wounds is obviously inhibited, and the healing speed of the wounds is improved.

Inventors

  • LI JIEJING
  • XU WEIJIE
  • TONG XUELI
  • CHEN JINZI
  • GUO XIAOFENG
  • LI LU

Assignees

  • 广州市东源药业科技有限公司
  • 东源药业科技(昆明)有限公司
  • 暨南大学附属第一医院（广州华侨医院）

Dates

Publication Date
20260505
Application Date
20260316

Claims (10)

  1. 1. The aloe polysaccharide is used in preparing product for promoting phagocytic ability of macrophage.
  2. 2. The use according to claim 1, wherein the aloe polysaccharide is aloe polysaccharide solution obtained by grinding aloe gel, stirring at 4-15 ℃, light-treating for at least 24 hours, and evaporating and collecting aloe polysaccharide pervaporation membrane on the surface of the solution through 75-95 ℃.
  3. 3. The use according to claim 1, wherein the illumination treatment is a normal white light illumination treatment, the white light having a wavelength of 390nm to 780nm.
  4. 4. The method for preparing phagocytic macrophages in vitro is characterized by comprising the step of adding aloe polysaccharide into a macrophage culture medium, wherein the aloe polysaccharide is aloe polysaccharide feed liquid obtained by grinding aloe gel, stirring at 4-15 ℃, carrying out light treatment for at least 24 hours, and evaporating and collecting aloe polysaccharide pervaporation membranes on the surface of the liquid through membranes at 75-95 ℃.
  5. 5. The method of claim 4, wherein the illumination process is a normal white light illumination process, and the white light has a wavelength of 390nm to 780nm.
  6. 6. The method of claim 5, wherein aloe polysaccharides are added to the culture medium in a ratio of 200mg/L to 800 mg/L.
  7. 7. The phagocytic macrophage produced by the method of claim 5 or 6.
  8. 8. Use of phagocytic macrophages prepared according to the method of claim 5 or 6 for the preparation of a medicament for promoting wound healing.
  9. 9. The use according to claim 8, wherein the medicament is in the form of an injection.
  10. 10. A medicament for promoting wound healing, comprising phagocytic macrophages prepared by the method of claim 5 or 6.

Description

Application of aloe polysaccharide in-vitro regulated phagocytic macrophages in promoting wound healing Technical Field The invention belongs to the field of application of aloe polysaccharide, and in particular relates to application of aloe polysaccharide in-vitro regulated phagocytic macrophages in promoting wound healing. Background Wound healing is generally divided into a bleeding phase, an inflammatory phase, a proliferation phase and a remodeling phase, and it is generally considered that the proper early transition of the inflammatory phase to the proliferation phase is a key to ideal repair, and in this process, macrophages serve as core regulators, and drive the repair process through phenotypic conversion and multifunctional secretion in different phases. In the inflammatory phase, macrophages first recruit neutrophils to augment immune defenses with pro-inflammatory (M1) phenotypes, phagocytosing pathogens and necrotic tissue, releasing ROS and proteases such as MMP-9 to break down damaged matrix, and secreting pro-inflammatory factors such as TNF-alpha, IL-1 beta, etc. In the later stage, macrophages initiate a key phenotypic shift, polarize to repair (M2), secrete anti-inflammatory factors such as IL-10, TGF-beta and the like to inhibit excessive inflammation, prevent secondary damage to healthy tissues and pave the way for the proliferation stage. If this stage is delayed (e.g. an increased inflammatory phase in a diabetic scald leading to an increased wound healing period), there will be a significant risk of chronic ulcers. In the proliferation stage, M2 macrophage becomes repair engine, and through secreting powerful angiogenesis promoting signals of Vascular Endothelial Growth Factor (VEGF), fibroblast growth factor (FGF-2) and the like, capillary angiogenesis is accelerated, wound hypoxia is improved, transforming growth factor-beta (TGF-beta), epidermal Growth Factor (EGF) and the like are released to activate fibroblast migration and proliferation, III type collagen deposition is promoted to form granulation tissue, and epidermal stem cell differentiation is stimulated to promote re-epithelialization process (the speed can be increased by 40%). Macrophages in this stage also transmit signaling molecules such as miRNA through exosomes, coordinating intercellular communication to optimize tissue reconstruction efficiency. The remodeling stage involves the replacement of collagen type, the transformation of macrophages to myofibroblasts to contract the wound surface, the subsequent elimination of superfluous repair cells by apoptosis signals (such as Fas ligand), degradation of type III collagen and promotion of type I collagen cross-linking remodeling by balancing the secretion of Matrix Metalloproteinases (MMP) and Their Inhibitors (TIMP), enhancement of tissue strength, and at the same time, mediation of vascular degeneration and water metabolism, promotion of scar softening maturation. If the regulation is unbalanced at this stage (such as TGF-beta 1 is continuously expressed in high level), pathological scar hyperplasia is caused. Disclosure of Invention In view of this, the invention proposes an application of aloe polysaccharide in vitro regulated phagocytic macrophages in promoting wound healing, which uses aloe polysaccharide to treat macrophages, polarize the macrophages into phagocytic macrophages, promote the production of anti-inflammatory cytokines and remove pathogenic microorganisms, necrotic tissues, cell debris or foreign bodies through the phagocytic function of the macrophages, and finally uses the cells to treat wounds by adopting an intradermal injection mode. Unlike available cell treating method with autologous cell or mesenchymal stem cell, the present invention adopts aloe polysaccharide extracted from natural plant to induce phagocytic macrophage, and the secreted anti-inflammatory cytokine, such as IL-10, is used in fast inflammation stage and the secreted growth factor and metalloprotease promote the healing quality and efficiency in proliferation stage and remodelling stage. The preparation method of aloe polysaccharide refers to a method for promoting aloe polysaccharide with a patent publication number of CN118240106A to form a pervaporation membrane, and specifically comprises the following steps: Removing two ends of fresh aloe vera leaves, soaking for 1-2 days with pure water, washing, removing the epidermis to obtain aloe gel to be ground, shearing the aloe gel to be ground in an automatic gel grinding device to finish the gel grinding, and obtaining aloe polysaccharide feed liquid. And placing aloe polysaccharide feed liquid into a stirring tank, stirring at 4-15 ℃ and carrying out illumination treatment for at least 24 hours, and then collecting aloe polysaccharide pervaporation membranes on the surface of the liquid in a high-temperature film forming tank at 75-95 ℃. In the invention, the illumination treatment is common white light illumination tre