CN-121975752-A - Enzyme preparation combination of dehydrogenase cascade reaction and application thereof

Abstract

The invention provides an enzyme preparation combination of dehydrogenase cascade reaction and application thereof, relating to the technical field of cascade reaction, wherein the enzyme preparation combination is formed by integrating a double-enzyme cascade catalytic reaction system of dehydrogenase and coenzyme thereof on a nano structure of a Schottky junction and is used for an electrode interface of a biosensor, so that the technical problems of low catalytic efficiency, high working voltage, more byproduct generation and large enzyme consumption of the dehydrogenase in the prior art are solved, the catalytic efficiency of the dehydrogenase and the coenzyme thereof is improved, and an equivalent-magnitude current signal is generated under the condition of less enzyme consumption, thereby reducing the production cost of test strips based on the electrochemical detection of the dehydrogenase, such as blood sugar, lactic acid and the like.

Inventors

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Assignees

• 广东乐心医疗电子股份有限公司

Dates

Publication Date

20260505

Application Date

20260128

Claims (10)

1. 1. The enzyme preparation combination of dehydrogenase cascade reaction is characterized in that the enzyme preparation combination is formed by integrating a double-enzyme cascade catalytic reaction system of dehydrogenase and coenzyme thereof on a nano structure of a Schottky junction, and is used for an electrode interface of a biosensor.
2. 2. The enzyme preparation combination according to claim 1, wherein the dehydrogenase and its coenzyme are glucose dehydrogenase and its coenzyme, or lactate dehydrogenase and its coenzyme.
3. 3. The enzyme preparation combination according to claim 2, wherein the electric field in the cascade of catalytic reaction systems is directed to promote electron flow from the coenzyme to the electrode, thereby lowering the operating voltage and reducing by-product formation in the reaction.
4. 4. The enzyme preparation combination according to any one of claims 1-3, wherein the schottky junction material is formed by combining a noble metal material and a semiconductor material; the noble metal material includes gold, platinum, and palladium; The semiconductor material includes titanium dioxide, cadmium oxide, zinc oxide, and zinc sulfide.
5. 5. The enzyme preparation combination of claim 4, wherein the method for preparing the schottky junction material comprises at least one of an in situ growth method and a loading method.
6. 6. The enzyme preparation combination according to claim 5, wherein the in situ growth method comprises the steps of: And adding a metal precursor in the synthesis process of the semiconductor nanocrystal to perform one-step reaction, and generating a semiconductor interface and a metal structure to obtain the material of the Schottky junction.
7. 7. The enzyme preparation combination according to claim 5, wherein the loading method comprises the steps of: and loading metal on the semiconductor nano material by a dipping reduction method to obtain the material of the Schottky junction.
8. 8. The enzyme preparation combination according to claim 4, wherein the dehydrogenase is immobilized on the surface of the schottky junction by charge adsorption or covalent bonding.
9. 9. Use of an enzyme preparation combination according to any one of claims 1-8 for the preparation of a blood glucose monitoring product or a lactic acid monitoring product.
10. 10. The use of claim 9, wherein the blood glucose monitoring product comprises a blood glucose test strip product; The lactic acid monitoring product comprises a lactic acid test strip product.

Description

Enzyme preparation combination of dehydrogenase cascade reaction and application thereof Technical Field The invention relates to the technical field of cascade reactions, in particular to an enzyme preparation combination of a dehydrogenase cascade reaction and application thereof. Background In modern blood glucose monitoring, glucose dehydrogenase-based detection test strips have become one of the mainstream technologies due to their good anti-interference performance and accuracy. The reaction area of the test paper strip is fixed with high-efficiency glucose dehydrogenase and coenzyme, after the blood sample is dripped, glucose in the blood can be subjected to specific catalytic reaction with the enzyme, the glucose dehydrogenase accurately catalyzes the oxidation of glucose, meanwhile, the coenzyme in the reduced state can be reacted with a chemical mediator which is designed in advance in the reaction area, the mediator can be diffused to the surface of an electrode after being reduced and oxidized under the applied low voltage, so that a weak current is generated, and the current is proportional to the concentration of glucose in the blood. Because glucose dehydrogenase is more expensive than traditional oxidase, it is currently highly desirable to improve the catalytic efficiency of glucose dehydrogenase and reduce the enzyme dosage per test strip, thereby improving the competitiveness of blood glucose test strip products. In view of this, the present invention has been made. Disclosure of Invention The invention aims to provide an enzyme preparation combination for a dehydrogenase cascade reaction, which can solve the technical problems of low catalytic efficiency, high working voltage, more byproduct generation and large enzyme consumption of the dehydrogenase in the prior art, improves the catalytic efficiency of the dehydrogenase and coenzyme thereof, and realizes the generation of an equal-magnitude current signal under the condition of less enzyme consumption, thereby reducing the production cost of test strips based on the electrochemical detection of the dehydrogenase, such as blood sugar, lactic acid and the like. The second purpose of the invention is to provide an application of an enzyme preparation combination of a dehydrogenase cascade reaction, which is beneficial to reducing the production cost of test strips such as blood sugar and lactic acid, and is beneficial to reducing the working potential and byproducts and improving the detection selectivity of the test strips by improving the directional transmissibility of enzyme catalytic reaction. In order to achieve the above object of the present invention, the following technical solutions are specifically adopted: In a first aspect, an enzyme preparation combination of a dehydrogenase cascade reaction is formed by integrating a double-enzyme cascade catalytic reaction system of a dehydrogenase and a coenzyme thereof on a nano structure of a schottky junction, and is used for an electrode interface of a biosensor. Further, the dehydrogenase and the coenzyme thereof are glucose dehydrogenase and the coenzyme thereof, or lactate dehydrogenase and the coenzyme thereof. Further, the electric field direction in the cascade catalytic reaction system is to promote electrons to flow from the coenzyme to the electrode, thereby reducing the working voltage and reducing the generation of byproducts in the reaction. Further, the material of the Schottky junction is formed by combining a noble metal material and a semiconductor material; the noble metal material includes gold, platinum, and palladium; The semiconductor material includes titanium dioxide, cadmium oxide, zinc oxide, and zinc sulfide. Further, the preparation method of the material of the Schottky junction comprises at least one of an in-situ growth method and a loading method. Further, the in-situ growth method comprises the following steps: And adding a metal precursor in the synthesis process of the semiconductor nanocrystal to perform one-step reaction, and generating a semiconductor interface and a metal structure to obtain the material of the Schottky junction. Further, the loading method comprises the following steps: and loading metal on the semiconductor nano material by a dipping reduction method to obtain the material of the Schottky junction. Further, the dehydrogenase is immobilized on the surface of the schottky junction by charge adsorption or covalent bonding. In a second aspect, the use of an enzyme preparation combination according to any one of the preceding claims for the preparation of a blood glucose monitoring product or a lactic acid monitoring product. Further, the blood glucose monitoring product comprises a blood glucose test strip product; The lactic acid monitoring product comprises a lactic acid test strip product. Compared with the prior art, the invention has at least the following beneficial effects: The enzyme preparation combination of the dehydrog