CN-121975754-A - Mesotrione nitroreductase, and encoding gene and application thereof

Abstract

The invention discloses a novel mesotrione nitroreductase Wmnrs, a coding gene wmnrs and application thereof, wherein the mesotrione nitroreductase can degrade mesotrione into 2-amino-4-methylsulfonyl benzoic acid (AMBA) to lose activity. The invention can cultivate the plants with the mesotrione-resistant transgene after the genes are introduced into the plants. The mesotrione nitroreductase Wmnrs and the coding gene thereof have potential application value in the biological repair of mesotrione residual pollution.

Inventors

• ZHANG BAOLONG
• Wan Minglai
• HE JIAN
• HOU XIAODONG
• CHEN LE
• LI CHENGHUI

Assignees

• 江苏省农业科学院

Dates

Publication Date

20260505

Application Date

20260204

Claims (10)

1. 1. The mesotrione nitroreductase Wmnrs is characterized in that the amino acid sequence is shown as SEQ ID NO. 2.
2. 2. The mesotrione nitroreductase encoding gene wmnrs of claim 1, wherein the nucleotide sequence is shown in SEQ ID NO. 1.
3. 3. An expression cassette, recombinant vector, recombinant cell or recombinant bacterium comprising the gene wmnrs of claim 2.
4. 4. The recombinant vector according to claim 3, wherein the recombinant vector is obtained by introducing the coding gene wmnrs according to claim 2 into a plasmid.
5. 5. The recombinant bacterium according to claim 3, wherein the recombinant bacterium is obtained by introducing the recombinant vector according to claim 3 or 4 into a host bacterium.
6. 6. The recombinant bacterium according to claim 5, wherein the host bacterium is Escherichia coli or Agrobacterium.
7. 7. Use of mesotrione nitroreductase Wmnrs of claim 1, encoding gene wmnrs of claim 2, expression cassette of claim 3, recombinant vector, recombinant cell or recombinant bacterium, recombinant vector of claim 4, recombinant bacterium of claim 5 or 6 in herbicide resistance of plants.
8. 8. Use of the mesotrione nitroreductase Wmnrs of claim 1, the coding gene wmnrs of claim 2, the expression cassette, recombinant vector, recombinant cell or recombinant bacterium of claim 3, the recombinant vector of claim 4, the recombinant bacterium of claim 5 or 6 in the degradation and detoxification and/or repair of residual contamination organisms of mesotrione.
9. 9. A method for obtaining a herbicide resistant plant comprising the steps of: 1) Allowing the plant to express the mesotrione nitroreductase Wmnrs of claim 1, or 2) The plant is made to contain the coding gene wmnrs of claim 2, and preferably the method of obtaining comprises the steps of transgene, crossing, backcrossing or asexual reproduction.
10. 10. A method for identifying whether a plant obtained by the method of claim 9 is herbicide resistant, comprising the steps of: 1) Identifying whether the plant expresses mesotrione nitroreductase Wmnrs of claim 1, or 2) Identifying whether said plant contains the coding gene wmnrs of claim 2.

Description

Mesotrione nitroreductase, and encoding gene and application thereof Technical Field The invention belongs to the technical field of agricultural biology, and relates to mesotrione nitroreductase, and a coding gene and application thereof. Background The herbicide-resistant transgenic technology ensures the safe production of crops, realizes the efficient and low-cost prevention and control of farmland weeds, and obviously changes the traditional weed control mode. By 2023, the global transgenic crop planting area reaches 2.063 hundred million hectares, wherein the glyphosate-resistant transgenic variety takes the dominant position, and the planting area reaches about 1.85 hundred million hectares, accounting for more than 90% of the total planting area of the transgenic crop. However, long-term large-scale use of glyphosate has accelerated the evolution of weed resistance, and more than 40 major farmland weeds have been reported to develop resistance to glyphosate worldwide, severely threatening their sustained control. In this context, the development and application of herbicides with different mechanisms of action, and the simultaneous construction of corresponding herbicide-resistant transgenic crops, is considered as an effective strategy for alleviating the problem of weed resistance. Therefore, the system explores the resistance gene resources of various herbicides, and has important significance for constructing novel herbicide-resistant transgenic crops. P-hydroxyphenylpyruvate dioxygenase (4-hydroxyphenylpyruvate dioxygenase, HPPD, EC 1.13.11.27) is a key enzyme in the tyrosine metabolic pathway in aerobic organisms. Firstly, tyrosine is catalyzed by tyrosine aminotransferase (tyrosine aminotransferase, TAT) to produce p-hydroxyphenylpyruvic acid (4-hydroxyphenylpyruvic acid, 4-HPP), and then the 4-HPP is converted into homogentisate (homogentisic acid, HGA) under the catalysis of HPPD. Urea-black acid is an important precursor for biosynthesis of plastoquinone and tocopherol in plants, wherein plastoquinone is a cofactor for photosynthesis, promoting synthesis of carotenoids, and tocopherol plays an important role in enhancing stress resistance of plants. HPPD inhibitor herbicides inhibit HPPD enzyme activity in a competitive manner, so that normal metabolism of tyrosine in plants is blocked, and carotenoid in plants is deficient, so that photooxidation of chlorophyll is reduced, photosynthesis of plants is affected, and albino symptoms are caused to die. Therefore, researchers have important instructive significance in developing new herbicides using HPPD as a new herbicide target enzyme. HPPD inhibitor herbicides can be classified according to chemical structure into triones (e.g., mesotrione, cyclosulcotrione, bicyclosulcotrione, etc.), pyrazolones (e.g., topramezone, sulfonyloxaziram, pyrazolte, etc.), isoxazolones (e.g., isoxazodone). Among them, mesotrione (mesotrione), also called mesotrione, is a trione herbicide developed in 1984 by the company Zhengda (Syngenta), has broad-spectrum herbicidal activity, and can effectively control most broadleaf weeds and part of grassy weeds. Although mesotrione has the advantages of high efficiency, low toxicity, good crop selectivity, high environmental compatibility and the like, the problem of resistance of field weeds to such herbicides is increasingly prominent with long-term and large-area application, and continuous effective application of the mesotrione is challenging. Therefore, the novel mesotrione degradation detoxification or resistance related genes are excavated and obtained, and according to the development of the developed transgenic crops, the mesotrione degradation or resistance related genes have important theoretical significance and practical application value for delaying resistance evolution, guaranteeing herbicide application safety and promoting sustainable development of modern agriculture. Disclosure of Invention The invention aims to solve the technical problem of the mesotrione nitroreductase Wmnrs with the mesotrione degradation function. The invention also solves the technical problem of providing the coding gene wmnrs of the mesotrione nitroreductase Wmnrs. The invention also solves the technical problem of providing an expression cassette, a recombinant vector, a recombinant cell or a recombinant bacterium containing the coding gene wmnrs. The invention also solves the technical problem of providing the mesotrione nitroreductase Wmnrs, the coding gene wmnrs, the expression cassette, the recombinant vector, the recombinant cell or the application of the recombinant bacteria in herbicide resistance of plants. The invention also solves the technical problem of providing the mesotrione nitroreductase Wmnrs, the coding gene wmnrs, and the application of the expression cassette, the recombinant vector, the recombinant cell or the recombinant bacterium in mesotrione degradation detoxification and/or repair. The technical pro