CN-121975805-A - Small-molecule RNA miR-7 and application thereof in prevention and control of diaphorina citri

Abstract

The invention discloses a small molecular RNA and application thereof in preventing and controlling diaphorina citri. The inventor finds that miRNA of the diaphorina citri, namely RNA miR-7, has a nucleotide sequence of 5'-UGAACACAGCUGGUGGUAUCUC-3' and is highly expressed in the ovary and the head of the diaphorina citri. Through the application of the miR-7 agonist, the spawning quantity of the diaphorina citri is obviously reduced, the ovarian dysplasia is abnormal, the titer of yellow dragon bacteria in the ovary is obviously reduced, and the method has good application prospect for preventing and controlling the diaphorina citri and further preventing and controlling the yellow dragon disease.

Inventors

• HE YURONG
• YUAN WEIWEI
• NIAN XIAOGE
• Zhang Songdou
• CEN YIJING

Assignees

• 华南农业大学
• 广东省农业科学院植物保护研究所

Dates

Publication Date

20260505

Application Date

20260114

Claims (9)

1. 1. The application of the small molecular RNA miR-7 in the prevention and treatment of diaphorina citri is characterized in that the nucleotide sequence of the small molecular RNA miR-7 is shown as SEQ ID NO. 4.
2. 2. The use according to claim 1, characterized by the use of miR-7 for promoting the expression of small-molecule RNA for controlling diaphorina citri.
3. 3. The use according to claim 2, wherein the diaphorina citri is a diaphorina citri female.
4. 4. The use according to claim 3, characterized by the use of miR-7 for increasing the micro-molecular RNA for reducing the oviposition of diaphorina citri females, for inhibiting the development of diaphorina citri females ovaries, and for reducing the titre of yellow dragon bacteria in diaphorina citri females ovaries.
5. 5. The use of claim 2, wherein the increasing the expression level of the small molecule RNA miR-7 is increased by feeding a agomir agonist targeting the small molecule RNA miR-7.
6. 6. The use of claim 5, wherein the concentration of miR-7 in the agomir agonist targeting small molecule RNA miR-7 is 7.5 μm.
7. 7. The preparation for preventing and treating the diaphorina citri is characterized by being agomir agonist targeting small-molecule RNA miR-7, and the nucleotide sequence of the small-molecule RNA miR-7 is shown as SEQ ID NO. 4.
8. 8. Use of the formulation of claim 7 for controlling diaphorina citri.
9. 9. The use according to claim 8, wherein the diaphorina citri is diaphorina citri female.

Description

Small-molecule RNA miR-7 and application thereof in prevention and control of diaphorina citri Technical Field The invention belongs to the technical field of pest control, and particularly relates to a small-molecule RNA miR-7 and application thereof in controlling diaphorina citri. Background The etiology of citrus yellow dragon disease is a gram-negative bacterium that is parasitic on the phloem of plants, with asian species (Candidatus Liberibacter asiaticus, CLas) being most widely distributed and causing the greatest hazard. Diaphorina citri is the main transmission medium of diaphorina citri, and CLas forms a reciprocal relationship with diaphorina citri in long-term evolution. CLas circulates and diffuses in the psyllids, avoids the immune system, and is beneficial to the propagation of germs of the psyllids. After diaphorina citri infects yellow dragon disease, the reproductive capacity of the diaphorina citri is enhanced, the spawning period is shortened, and the spawning period is prolonged. The adaptability of the psyllids with bacteria is enhanced along with the increase of fertility, and the bacteria can be transmitted to offspring through ovaries. However, regarding how the diaphorina citri regulates the reproduction of diaphorina citri to realize the molecular mechanism of win-win, the molecular mechanism is not reported so far. However, as the citrus yellow-tailed bacteria cannot be cultivated manually at present, it is difficult to directly formulate effective prevention and treatment measures for the bacteria. MiRNAs are small-molecule non-coding RNAs widely existing in eukaryotes, participate in the regulation of gene expression after transcription, and have important influence on various physiological processes of insects. Therefore, the miRNA-based control strategy has wide application prospect in diaphorina citri. Disclosure of Invention Based on the defects and shortcomings of the prior art, the invention aims to provide small-molecule RNA miR-7 and application thereof in controlling diaphorina citri. The first aim of the invention is to provide application of small molecular RNA miR-7 in prevention and treatment of diaphorina citri, and the nucleotide sequence of the small molecular RNA miR-7 is shown as SEQ ID NO. 4. Preferably, the application is the application of promoting the expression of the small molecular RNA miR-7 in the prevention and treatment of diaphorina citri. Preferably, the diaphorina citri is a diaphorina citri female insect. Preferably, the application is the application of improving the micro-molecular RNA miR-7 in reducing the spawning quantity of the diaphorina citri, inhibiting the development of the ovary of the diaphorina citri and reducing the titer of the yellow dragon germ in the ovary of the diaphorina citri. The miR-7 influences the reproductive development of the diaphorina citri by reducing the titer of the diaphorina citri, so that the diaphorina citri is prevented and treated, and the transmission of the diaphorina citri is inhibited. Preferably, the expression level of the small-molecule RNA miR-7 is improved by feeding agomir agonist targeting the small-molecule RNA miR-7. Preferably, the concentration of miR-7 in the agomir agonist of the targeting small molecule RNA miR-7 is 7.5 mu M. The second purpose of the invention is to provide a preparation for preventing and treating diaphorina citri, the preparation is agomir agonist of targeted small-molecule RNA miR-7, and the nucleotide sequence of the small-molecule RNA miR-7 is shown as SEQ ID NO. 4. A third object of the invention is to provide the use of said formulation for controlling diaphorina citri. Preferably, the diaphorina citri is a diaphorina citri female insect. The invention has the beneficial effects that: the invention discovers that miRNA of diaphorina citri, namely miR-7, is highly expressed in the ovary and head of diaphorina citri. Through the application of the miR-7 agonist, the spawning quantity of the diaphorina citri is reduced, the ovarian dysplasia is caused, the titer of yellow dragon bacteria in the ovary is obviously reduced, the control effect on the diaphorina citri nymphs is obvious, and the application prospect is good. Drawings FIG. 1 is a spatial-temporal expression profile of diaphorina citri miR-7. The expression level of miR-7 in different tissues of the diaphorina citri females is shown in A, different letters show that the expression level difference of miR-7 in different tissues is obvious (p < 0.05), B shows that the expression level of miR-7 in different development stages of the diaphorina citri females (CLas +) and the non-diaphorina citri females (CLas-) is not obvious (p > 0.05), ns show that the expression level difference of miR-7 in the diaphorina citri females and the non-diaphorina citri females in the same development stage is obvious, and the expression level difference of miR-7 in the diaphorina citri females and the non-diaphorina citri fema