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CN-121975806-A - Application of miR-184 in regulating reproduction of female diaphorina citri

CN121975806ACN 121975806 ACN121975806 ACN 121975806ACN-121975806-A

Abstract

The invention discloses miR-184 and application thereof in controlling reproduction of female diaphorina citri. The nucleotide sequence of the small molecule miR-184 provided by the invention is shown as SEQ ID NO.4, and is highly expressed in ovaries of diaphorina citri females. Through the application of the agonist fed with miR-184, the spawning quantity of the diaphorina citri is obviously reduced, the development of ovaries is inhibited, the titer of yellow dragon bacteria in the ovaries is obviously reduced, and the method has good application prospects in the aspects of regulating and controlling the reproduction of the diaphorina citri females, preventing and controlling the diaphorina citri females, preventing yellow dragon diseases and the like.

Inventors

  • HE YURONG
  • He Jielan
  • NIAN XIAOGE
  • Zhang Songdou
  • CEN YIJING

Assignees

  • 华南农业大学
  • 广东省农业科学院植物保护研究所

Dates

Publication Date
20260505
Application Date
20260114

Claims (9)

  1. 1. The application of the small molecular RNA miR-184 in the prevention and treatment of diaphorina citri is characterized in that the nucleotide sequence of the small molecular RNA miR-184 is shown as SEQ ID NO. 4.
  2. 2. The use according to claim 1, wherein the use is for promoting expression of small molecule RNA miR-184 in controlling diaphorina citri.
  3. 3. The use according to claim 2, wherein the diaphorina citri is a diaphorina citri female.
  4. 4. The use according to claim 3, characterized by the use of reducing the oviposition of diaphorina citri females, inhibiting the development of diaphorina citri females ovaries and reducing the titer of yellow dragon bacteria in diaphorina citri females ovaries for promoting the expression of small molecule RNA miR-184.
  5. 5. The use of claim 1, wherein said promoting expression of small RNA miR-184 is achieved by feeding a agomir agonist targeting small RNA miR-184.
  6. 6. The use of claim 5, wherein the concentration of small molecule RNA miR-184 in the agomir agonist targeting small molecule RNA miR-184 is 10 μm.
  7. 7. A preparation for preventing and controlling diaphorina citri is characterized by being agomir agonist of targeted small-molecule RNA miR-184, and the nucleotide sequence of the small-molecule RNA miR-184 is shown as SEQ ID NO. 4.
  8. 8. Use of the formulation of claim 7 for controlling diaphorina citri.
  9. 9. The use according to claim 8, characterized by the use of said formulation for controlling diaphorina citri females.

Description

Application of miR-184 in regulating reproduction of female diaphorina citri Technical Field The invention belongs to the technical field of pest control, and particularly relates to application of miR-184 in controlling reproduction of female diaphorina citri. Background The plant-derived species Phyllostachys citri (Diaphorina citriKuwayama) belongs to the family Phyllostachys of the order Henonis, is a main vector insect for citrus yellow dragon disease, and is an important pest in the young shoot phase of Rutaceae plants such as citrus Citrus reticulata, murraya paniculata Murraya exotica, clausena lansium Clausena lansium, etc. (Lai Jian Feng et al, 1989). Diaphorina citri is mainly distributed in the southern Yangtze province (Jiang Hongyan, etc., 2018) of Guangdong and Guangxi Provinces, guizhou, yunnan, jiangxi, etc. in China. The diaphorina citri obtains nutrition through the young leaves and young shoots of the piercing-sucking host plant, and the cluster sucking is a main hazard, so that the young shoots wither, the young leaves deform and the young shoots bend (Ruan Chuanqing and the like, 2012). The young shoots of the plants infected with the citrus yellow dragon disease are uniformly yellow, the old leaves are in a mottled shape (Fan Huaizhong et al, 1986), the fine roots are rotted and shed, the flowers are many tiny malformations, the fruit shape is light and is easy to shed, and the whole plants die after a few months to a few years of attack (Lin Kongxiang, 1956). In recent years, there are several reports that mirnas play a key regulatory role in reproductive development of insects. The targeted knockout mutation technology is utilized to systematically study the functions of miRNAs in Drosophila, a plurality of miRNAs participate in regulating and controlling the reproductive process of Drosophila through a direct or indirect way, the functional deficiency of miR-283, miR-304, miR-927 and the like can lead to the reduction of male fertility, the functional deficiency of miR-276b, miR-318, miR-994 and the like can lead to the reduction of female fertility (Chen et al, 2014), let-7 and miR-278 participate in a juvenile hormone (juvenile hormone, JH) signal path through targeting Kr-h1, so that the yolk production and oocyte maturation of migratory locust Locusta migratoria are regulated and controlled (Song et al, 2018), in Egyptian AEDES AEGYPTI, the reduction of the expression of miR-275 by using miRNA inhibitors can lead to digestion abnormality, so as to influence the maturation of the egg (Bryan et al, 2010), and the expression level of miR-2739 and miR-167 synergistically regulate and control the primordin receptor (vitellogenin receptor, R) in the ovarian development of domestic silkworm Bombyxmori. In diaphorina citri, the study outcome was relatively low. In reports of improving diaphorina citri fertility by using yellow dragon bacteria, miR-275 negatively regulates and controls a coding vitellogenin receptor, activates a juvenile hormone signal path, improves the fertility of diaphorina citri, promotes the replication of yellow dragon bacteria in ovaries (Nian et al., 2024), reduces the expression of miR-34 by improving the expression of a lipid hormone and a receptor thereof, promotes the lipid metabolism mobilization of diaphorina citri, activates the juvenile hormone signal path, and promotes the generation of yolk, thereby improving the fertility of diaphorina citri (Li et al., 2024). Although partial research results in the early stage initially identify and analyze the molecular mechanism of female diaphorina citri ovary development and reproduction, digging more ovary-specific small molecular RNAs of diaphorina citri and analyzing the influence of the small molecular RNAs on the diaphorina citri reproduction are the research basis of genetic prevention and control of diaphorina citri. Disclosure of Invention The diaphorina citri has strong reproductive capacity and is mainly harmful to tender leaf tips of the thorn-sucking host plants. Thus reducing or preventing the fertility of female diaphorina citri is one of the ways to effectively reduce its harm. Under natural environment, plant hosts containing miR-184 can enter the diaphorina citri body through feeding so as to influence reproduction of the diaphorina citri, and the purpose of pest control is achieved. Based on the existing defects and shortcomings, the invention aims to provide application of miR-184 in controlling reproduction of female diaphorina citri. The first purpose of the invention is to provide application of small-molecule RNA miR-184 in prevention and treatment of diaphorina citri, and the nucleotide sequence of the small-molecule RNA miR-184 is shown as SEQ ID NO. 4. Preferably, the application is the application of promoting the expression of the small molecular RNA miR-184 in the prevention and treatment of diaphorina citri. Preferably, the diaphorina citri is a diaphorina citri female insect. Pref