CN-121975813-A - Brown planthopper NlCPSF gene, coded protein thereof and application of dsRNA thereof in brown planthopper control

Abstract

The invention relates to the field of biotechnology, in particular to application of brown planthopper NlCPSF gene, coded protein thereof and dsRNA thereof in brown planthopper control. The invention discloses NlCPSF genes, which can cause the reduction of survival rate of brown planthoppers when the functions of the genes are inhibited, wherein the survival rate of brown planthoppers is obviously reduced and the morphological appearance is obviously changed after dsRNA (double-stranded ribonucleic acid) of NlCPSF100 is synthesized in vitro by a microinjection mode to silence NlCPSF genes. The invention has good application prospect and has important significance for preventing brown planthoppers.

Inventors

• Jing shengli
• XU JINGANG
• JIN JIANFENG
• LIU YALI
• WANG FEIFEI
• YANG JING
• LIU QINGSONG
• LI YANAN
• YU BIN

Assignees

• 信阳师范大学

Dates

Publication Date

20260505

Application Date

20260128

Priority Date

20251126

Claims (4)

1. 1. The brown planthopper NlCPSF gene is characterized in that the nucleotide sequence of the gene is shown as SEQ ID NO. 1 in a sequence table.
2. 2. The protein encoded by the brown planthopper NlCPSF gene according to claim 1, wherein the amino acid sequence of the protein encoded by the brown planthopper NlCPSF gene is shown as SEQ ID NO. 2 in the sequence table.
3. 3. The dsRNA of the brown planthopper NlCPSF gene according to claim 1, wherein the dsRNA sequence of the brown planthopper NlCPSF gene is shown in a sequence table SEQ ID NO. 3.
4. 4. Use of the dsRNA of the brown planthopper NlCPSF gene according to claim 3 for controlling brown planthopper.

Description

Brown planthopper NlCPSF gene, coded protein thereof and application of dsRNA thereof in brown planthopper control Technical Field The invention relates to the field of biotechnology, in particular to application of brown planthopper NlCPSF gene, coded protein thereof and dsRNA thereof in brown planthopper control. Background Rice is one of the most important staple foods for the population of China, and the increase of the yield is important. Among rice pests, brown planthoppers (NILAPARVATA LUGENS ST å l) are one of the most destructive insects, and the traditional control method mainly depends on chemical pesticides, so that the environment is destroyed, the human health is endangered, and the drug resistance of brown planthoppers is generated. RNAi technology, RNA interference technology (RNA INFERENCE, RNAI), is a technology that utilizes double-stranded RNA (dsRNA) induction to silence target gene mRNA. Because of the huge genome information of brown planthoppers and the large number of functional genes, the selection of which genes are silencing targets and the adoption of which RNAi technical means can achieve good control effects is a key point of the problem and is a difficulty in the technical field. The CPSF100 gene (CLEAVAGE AND Polyadenylation Specificity Factor) is an important multi-subunit complex component in eukaryotes, and is closely related to post-transcriptional processing of mRNA, particularly important in cleavage and tailing of the 3' -end of mRNA. It is involved in recognizing the polyadenylation signal of mRNA (polyadenylation signal, typically AAUAAA sequences) and catalyzing cleavage of the 3' end of mRNA by acting synergistically with other subunits such as CPSF73, CPSF160, fip1 and WDR 33. Specifically, CPSF100 plays a role in stabilizing the CPSF complex structure and is in close fit with CPSF73 to complete the 3 'end shearing process, and a polyadenylation tail is added to the 3' end to promote mRNA stability, nuclear export and translation efficiency. Currently, the CPSF100 gene is studied in model plants (e.g., arabidopsis, rice) and animals (e.g., human, mouse). In plants, mutations in CPSF100 may lead to embryonic lethality or dysplasia. However, the function of CPSF100 in brown planthoppers is not yet clear. Disclosure of Invention The invention aims to provide the brown planthopper NlCPSF gene, the coded protein and the application of dsRNA thereof in brown planthopper control so as to solve the problems in the background technology. The nucleotide sequence of the gene NlCPSF of brown planthopper provided by the invention is shown as SEQ ID NO. 1 in a sequence table. SEQ ID NO:1 ataaaatgac atctattata aaagtacatg ctctctctgg agctcatgat gagactcctc 60 catgctacat attacaagtt gatgagttca gattcttgtt ggattgcggc tggagtgaaa 120 cctttgatca agaatttgta aaagagctca aaagacatgt gaaccagatt gatgctgttc 180 tcctgtctta tcctgacccg ttgcatctgg gagctctgcc ctatattgtc ggcaaatgtg 240 gcctcaactg cccgatctac gccacgattc ctgtttataa aatgggtcaa atgttcatgt 300 atgacctata tcagtctcga catgatatgg aggagttcga aatgtttact ttagatgacg 360 ttgatgcagc ttttgacaaa attgtccaac tgaaatataa tcagagtgtt ccaatgaaag 420 gaaaaggatt tggcttaaca atagttccac taccagctgg gcatatgata ggtggcacag 480 tatggaaaat aatgaagata ggcgaggagg atataatata tgcagttgat ttcaatcata 540 aaaaggagcg acatctaaac ggttgtgagt tggagttcca gaggccgtcg cttctgatca 600 cagatgcttc aaatgccacc tatcagcaag ctaggagaag aacaagggat gaaaagttga 660 tgacaaatat tctgcaaacg ttgcgtaaca acggtaacgt tctggtggcc gtcgacacag 720 ctggtcgtgt tttagagctg gctcacatgt tggaccagct gtggaggaat aaggagtcag 780 gacttctggc ttactcgttg gccctcctca ataacgttag ctataatgta gtcgaattcg 840 caaaatcaca gattgagtgg atgagtgata aattgatgag gagttttgag ggcgctagaa 900 ataatccatt ccagttcaag catctgcagc tctgtcatag tatggctgaa ttaagcaaag 960 tttctagtcc taaggtcgtt ttagccagta caccagatat ggaatgtggt ttctcaaggg 1020 agctcttcct gcagtggagc tcacaagaaa ataatagtat tatcatcacc tccagaactt 1080 cacctggtac gttagctaga gatttaattg atctaggaac taatagaatg atacagctac 1140 aagtcaaaag gcgtgttcgt ttagagggcg ctgaacttct agagtatcat cggaaggaaa 1200 aagaagctaa ggacaaaaaa cctgataaga aaggtcttct agatgtgtac ataagttcag 1260 actccgaaga tgatttggag atactgaata tcaacagagg aaagcacgat cttatggtga 1320 aacacgagtt caacaagcag caaacaagtt ttttcaagaa cagcaaaaag cattatccta 1380 tattcccgtt ctttgaagcc aagatgaaat gcgacgagta tggcgaaata atcaagccag 1440 aagactacaa gctggcagat gtgaatcctg gaggtgaaga caacaaggag aactttgagg 1500 tgaaagagga ggatctaacg atagacatga ctgaggttcc caccaaatgc gtgtcgttca 1560 ccaaaacagt gcaggtttcc tgttcggtgc agttcattga tttcgagggt cggtcggatg 1620 gcgagtccat gatgaaaatc ctctcgcaac tgaaaccgag gcggttggtt ctagtgcgag 1680 gatcgcccga caacacagcg acaatggccg accagatcca acagtggaac gatatccgcg 1740 tgttcactcc tggcaagggt gatgtggtcg acgccaccac cgagagtcac atctaccaag 1800 tgagactgac cgatgcgttg atcagctcgt tggagttcca gaagggcaag aactctgagc 1860 tggcctggct cgatgcccag attgctgtca agagtaaggg cgtcgatgcc actccaatgg 1920 agactgatca ggacaatgaa aatgtcgccg aagatgaaat atatacctta gaacctttgc 1980 cagttcat