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CN-121975815-A - ZmNF-YA13 gene and application thereof in plant disease-resistant breeding

CN121975815ACN 121975815 ACN121975815 ACN 121975815ACN-121975815-A

Abstract

The invention relates to a ZmNF-YA13 gene and application thereof in plant disease-resistant breeding, which belong to the technical field of plant gene breeding, the nucleotide sequence of the ZmNF-YA13 gene is shown as SEQ ID NO.1, the amino acid sequence of protein coded by the gene is shown as SEQ ID NO.2, the ZmNF-YA13 gene can be used for regulating and controlling the resistance of plants to corn small spot disease, the over-expression of the ZmNF-YA13 gene can enhance the resistance of plants to corn small spot disease, and the functional deficiency of the ZmNF-YA13 gene can weaken the resistance of plants to corn small spot disease. The invention discovers and verifies the resistance of ZmNF-YA13 gene positive regulation plants to corn small spot disease, and provides a new gene target and an important theoretical basis for corn disease-resistant breeding.

Inventors

  • XIE SHANSHAN
  • JIANG HAIYANG
  • WU GUICHUN
  • SONG ZHENG
  • WANG YULU
  • Kong Zhenyang

Assignees

  • 安徽农业大学

Dates

Publication Date
20260505
Application Date
20260119

Claims (7)

  1. 1. A ZmNF-YA13 gene is characterized in that the nucleotide sequence of the ZmNF-YA13 gene is shown as SEQ ID NO.1, and the amino acid sequence of protein coded by the gene is shown as SEQ ID NO. 2.
  2. 2. The application of the ZmNF-YA13 gene in plant disease-resistant breeding is characterized in that the ZmNF-YA13 gene is used for regulating and controlling the resistance of plants to corn small spot disease (Southern corn leaf blight).
  3. 3. The method according to claim 2, wherein the ZmNF-YA13 gene positively regulates the resistance of a plant to maize leaf scald disease.
  4. 4. The method of claim 3, wherein the ZmNF-YA13 gene is overexpressed to increase the resistance of the plant to maize leaf scald disease.
  5. 5. The method of claim 3, wherein the ZmNF-YA13 gene is functionally deleted to impair the resistance of the plant to maize leaf scald disease.
  6. 6. The method according to claim 2, wherein the plant breeding process is performed by using genetic engineering technology to create a homozygous crop variety overexpressing the ZmNF-YA13 gene, thereby obtaining a crop variety with increased resistance to maize leaf spot.
  7. 7. The method of claim 2, wherein the plant is maize.

Description

ZmNF-YA13 gene and application thereof in plant disease-resistant breeding Technical Field The invention belongs to the technical field of plant gene breeding, and particularly relates to a ZmNF-YA13 gene and application thereof in plant disease-resistant breeding. Background Corn is susceptible to a variety of diseases during its growth period, with corn small spot (Southern corn leaf blight) caused by the fungus vermicularia on umbilicus (Bipolaris maydis) being a globally distributed foliar disease. Typical symptoms of maize leaf spot disease are the appearance of water-borne or chlorosis spots on the leaves, which later develop into yellow-brown oval lesions, which when severe lead to leaf death. In recent years, corn small spot disease has a trend of increasing popularity and causes huge corn yield loss due to the influence of factors such as planting mode adjustment, variety replacement, climate change and the like. At present, chemical control is still a main means for controlling the disease, but the problems of pathogenic bacteria resistance, pesticide residue, ecological environment destruction and the like are easily caused after long-term use. In contrast, breeding and utilizing disease resistant varieties is a fundamental approach to achieving green sustainable control. However, resistance of maize to small leaf spot is controlled by multiple genes and the genetic mechanism is complex. In the aspect of disease resistance genetic mechanism research, a plurality of QTLs related to the resistance of the small spot disease are identified in a corn genome by Quantitative Trait Locus (QTL) positioning in the prior art, but the functional genes which are successfully cloned are still limited. Such as ZmCCoAOMT2, which enhances resistance by modulating phenylpropane metabolism, zmFUT1 and MYBR92 identified by multiple sets of chemical strategies, and the like. Therefore, the existing disease-resistant germplasm resources related to corn small spot disease are still deficient, and the current excavation of disease-resistant gene resources cannot meet the breeding requirement, so that the invention provides a ZmNF-YA13 gene and application thereof in plant disease-resistant breeding. Disclosure of Invention The invention aims to solve the problems and provide a ZmNF-YA13 gene and application thereof in plant disease-resistant breeding. The invention realizes the above purpose through the following technical scheme: The invention provides a ZmNF-YA13 gene, the nucleotide sequence of the ZmNF-YA13 gene is shown as SEQ ID NO.1, and the amino acid sequence of protein coded by the gene is shown as SEQ ID NO. 2. The invention also provides application of the ZmNF-YA13 gene in plant disease-resistant breeding, and the ZmNF-YA13 gene is used for regulating and controlling the resistance of plants to corn small spot. As a further optimization scheme of the invention, the ZmNF-YA13 gene positively regulates the resistance of plants to corn small leaf spot (Southern corn leaf blight). As a further optimization of the invention, zmNF-YA13 gene is over-expressed to enhance the resistance of plants to maize leaf spot. As a further optimization of the invention, zmNF-YA13 gene functions are deleted to weaken the resistance of the plant to corn leaf spot. As a further optimization scheme of the invention, in the plant breeding process, a homozygous crop variety which over-expresses the ZmNF-YA13 gene is created by utilizing a genetic engineering technology, so that the crop variety with enhanced resistance to corn small spot is obtained. As a further optimization of the invention, the plant is corn. The invention has the beneficial effects that: According to the invention, the ZmNF-YA13 expression level of corn infected with corn small spot pathogen (Bipolaris maydis) is analyzed, the ZmNF-YA13 gene participates in the defense reaction of corn to the small spot pathogen, and the ZmNF-YA13 gene over-expression experiment and the ZmNF-YA13 gene knockout experiment prove that the ZmNF-YA13 can positively regulate and control the resistance of plants to the corn small spot disease, thus providing a new gene target and important theoretical basis for corn disease-resistant breeding. Drawings FIG. 1 is a graph showing the analysis of ZmNF-YA13 gene expression level of corn after infection with Leptosphaeria maydis (Bipolaris maydis); FIG. 2 shows the relative expression levels of ZmNF-YA13 in each line of ZmNF-YA13 transgenic maize; FIG. 3 shows the mutation sites and sequencing results of ZmNF-YA13 mutants; FIG. 4 shows the onset of ZmNF-YA13 overexpressing strain and mutant material after inoculation with ZmNF-YA13-OE and ZmNF-YA13-ko strain (A: representative image of disease symptoms at 7 days after inoculation with ZmNF-YA13-OE and ZmNF-YA13-ko strain, scale bar representing 1 cm; B: disease index of ZmNF-YA13-OE and ZmNF-YA13-ko strain after inoculation with ZmNF-YA 7 days; C: relative biomass of ZmNF-YA13-OE and ZmNF-Y