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CN-121975828-A - ZmDXS2 gene and application thereof in regulation and control of corn kernel size

CN121975828ACN 121975828 ACN121975828 ACN 121975828ACN-121975828-A

Abstract

The invention discloses ZmDXS gene and application thereof in regulating and controlling corn kernel size. The polynucleotide of ZmDXS gene is shown as SEQ ID No.1, and the protein sequence is shown as SEQ ID No. 2. The invention screens a material with smaller grains and lighter color from a corn EMS mutant library, and discovers that the material is caused by ZmDXS gene mutation through sequence analysis. Therefore, the invention starts from mutant materials, establishes a detailed technical route, combines the functions of researches ZmDXS2 such as molecular biology, cytology analysis, transcriptome analysis and the like through experiments such as phenotype observation, nutrient component measurement and the like, analyzes the molecular mechanism of ZmDXS affecting the development of corn kernels, and provides theoretical basis for improving the quality and the yield of corn.

Inventors

  • ZHANG LAN
  • WANG LEI
  • LUO YANZHONG
  • ZHENG HONGYAN
  • LIU YUAN

Assignees

  • 中国农业科学院生物技术研究所

Dates

Publication Date
20260505
Application Date
20260330

Claims (7)

  1. A zmxs 2 gene characterized in that the polynucleotide of ZmDXS2 is set forth in (a), (b), (c) or (d): (a) A polynucleotide shown as a sequence table SEQ ID No. 1 or (B) Polynucleotide which can hybridize with the complementary sequence of SEQ ID No. 1 under stringent hybridization condition, the protein encoded by the polynucleotide still has the function of regulating and controlling the size of corn kernels; (c) A polynucleotide having at least 90% or more homology to the polynucleotide shown in SEQ ID No. 1, or (D) The polynucleotide mutant obtained by deleting, substituting or inserting one or more bases on the basis of the polynucleotide shown in SEQ ID No. 1 still has the function of regulating the size of corn kernels.
  2. A zmxs 2 protein characterized in that the amino acid sequence of the ZmDXS protein is as set forth in (a), (b) or (c): (a) An amino acid sequence shown as a sequence table SEQ ID No. 2 or (B) Amino acid having at least 90% or more homology with the amino acid shown in SEQ ID No. 2, or (C) The protein mutant obtained by deleting, substituting or inserting one or more amino acids on the basis of the protein shown in SEQ ID No.2 still has the function of regulating the size of corn kernels.
  3. 3. A vector comprising the ZmDXS gene of claim 1.
  4. 4. An engineered bacterium comprising the vector of claim ZmDXS gene.
  5. 5. Use of ZmDXS gene according to claim 1 for breeding maize kernel size-controlling varieties.
  6. 6. A method for increasing corn kernel, wherein the expression level of ZmDXS protein in corn is increased.
  7. The interacting protein MNB1a protein of ZmDXS2 protein is characterized in that the amino acid sequence of the MNB1a protein is shown as a sequence table SEQ ID No. 3.

Description

ZmDXS2 gene and application thereof in regulation and control of corn kernel size Technical Field The invention belongs to the field of plant genetic engineering and biotechnology, and particularly relates to ZmDXS gene and application thereof in regulation of corn kernel size. Background Corn (Zea mays) is one of the globally important food crops, whose grain development and accumulation of nutrients have a significant impact on corn yield and quality. 1-deoxy-D-xylulose 5-phosphate synthase (DXS) is a rate-limiting enzyme in the methylerythritol 4-phosphate (MEP) pathway, and GGPP, a precursor substance for the synthesis of various plant secondary metabolites including vitamin E and carotenoids, comes from the MEP pathway, so that the DXS gene plays an important role in the synthesis of vitamin E and provitamin A, which are essential to humans. The DXS gene was originally isolated in E.coli, and cloning and functional studies have been subsequently performed in a variety of plants including Arabidopsis thaliana (Arabidopsis thaliana), piper nigrum, and peppermint (Mentha haplocalyx) (SPRENGER ET et al, 1997; LOIS et al, 1998; LANGE et al, 1998). As key rate-limiting enzymes of the MEP pathway DXS plays a central metabolic role, the expression level of which directly influences the accumulation of important isoprenoids downstream, including Carotenoids (Carotenoids), vitamin E (Vitamin E, VE), abscisic acid (Abscisic acid, ABA), gibberellins (Gibberellins, GA) and monoterpenes (Monoterpenoids), etc., DXS protein abundance affects the content of these metabolites (ESTEVEZ et al., 2001; MUNOZ-BERTOMEU et al., 2006; WRIGHT et al., 2014), as in arabidopsis, overexpression of DXS significantly increases the levels of isoprenoids such as plant chlorophyll, tocopherol, carotenoids, ABA and GA (ESTEVEZ et al., 2001). At present, the research on the function of DXS genes is mainly focused on mode plants such as arabidopsis thaliana, tomato and the like, and the role of the DXS genes in isoprenoid metabolic pathways is mainly analyzed. However, in important crops (such as rice, wheat, corn, etc.), the DXS gene research system is not yet complete. Therefore, the biological functions of the crop DXS genes in important crops and the regulation network thereof are deeply explored, so that the gap in the field can be filled, and new theoretical basis and gene resources can be provided for genetic improvement and quality improvement of crops. Disclosure of Invention The invention aims to provide ZmDXS gene and application thereof in regulating and controlling the size of corn kernels. ZmDXS2 gene, wherein the polynucleotide of ZmDXS2 is represented by (a), (b), (c) or (d): (a) A polynucleotide shown as a sequence table SEQ ID No. 1 or (B) Polynucleotide which can hybridize with the complementary sequence of SEQ ID No. 1 under stringent hybridization condition, the protein encoded by the polynucleotide still has the function of regulating and controlling the size of corn kernels; (c) A polynucleotide having at least 90% or more homology to the polynucleotide shown in SEQ ID No. 1, or (D) The polynucleotide mutant obtained by deleting, substituting or inserting one or more bases on the basis of the polynucleotide shown in SEQ ID No. 1 still has the function of regulating the size of corn kernels. ZmDXS2 protein, wherein the amino acid sequence of the ZmDXS protein is shown as (a), (b) or (c): (a) An amino acid sequence shown as a sequence table SEQ ID No. 2 or (B) Amino acid having at least 90% or more homology with the amino acid shown in SEQ ID No. 2, or (C) The protein mutant obtained by deleting, substituting or inserting one or more amino acids on the basis of the protein shown in SEQ ID No.2 still has the function of regulating the size of corn kernels. A vector comprising the ZmDXS gene. An engineering bacterium comprising the vector of ZmDXS gene. The ZmDXS gene is applied to cultivating varieties for regulating and controlling the size of corn kernels. A method for increasing corn kernel and improving ZmDXS protein expression level in corn. The interaction protein MNB1a of ZmDXS proteins, and the amino acid sequence of the MNB1a protein is shown as a sequence table SEQ ID No. 3. The invention has the beneficial effects that a material with smaller grains and lighter color is screened from the corn EMS mutant library, and is found to be caused by ZmDXS gene mutation through sequence analysis. Therefore, the invention starts from mutant materials, establishes a detailed technical route, combines the functions of researches ZmDXS2 such as molecular biology, cytology analysis, transcriptome analysis and the like through experiments such as phenotype observation, nutrient component measurement and the like, analyzes the molecular mechanism of ZmDXS affecting the development of corn kernels, and provides theoretical basis for improving the quality and the yield of corn. Drawings FIG. 1 is a schematic representation of th