Search

CN-121975839-A - Application of GSN7 protein or coding gene thereof in regulating and controlling appearance quality of plant seeds

CN121975839ACN 121975839 ACN121975839 ACN 121975839ACN-121975839-A

Abstract

The invention provides application of GSN7 protein or a coding gene thereof in regulating and controlling the appearance quality of plant seeds, and the invention firstly defines the regulating and controlling relation between GSN7 gene and the appearance quality of the plant seeds, can obviously improve the appearance quality of the plant seeds by reducing the expression level of GSN7 gene or the expression level or activity of the GSN7 gene, increases the grain length, reduces the grain width and the length-width ratio, reduces the chalkiness rate and simultaneously improves the transparency. The GSN7 gene and the application thereof provided by the invention can be used for improving plant germplasm resources, and have important popularization and application values in the aspect of cultivating new varieties of high-quality plants.

Inventors

  • WU YUNZHE
  • LIU XUEYING
  • HU JIE
  • WANG QI
  • WANG YUN
  • WU KUN
  • FU XIANGDONG

Assignees

  • 中国科学院遗传与发育生物学研究所

Dates

Publication Date
20260505
Application Date
20260113

Claims (10)

  1. Application of GSN7 protein, or coding gene thereof, or biological material containing coding gene thereof in negative regulation of appearance quality of plant seeds, characterized in that GSN7 protein comprises any one of the following amino acid sequences: (1) An amino acid sequence as shown in SEQ ID NO.3 or 6; (2) The amino acid sequence with the same functional protein obtained by replacing, inserting or deleting one or more amino acids in the amino acid sequence shown in the (1).
  2. GSN7 protein, or encoding gene thereof, or application of biological material containing the encoding gene in any one of the following (1) - (3): (1) Regulating and controlling the grain shape of plant grains; (2) Regulating and controlling the chalkiness rate of plant seeds; (3) Regulating and controlling the transparency of plant seeds; the GSN7 protein comprises any one of the following amino acid sequences: (1) An amino acid sequence as shown in SEQ ID NO.3 or 6; (2) The amino acid sequence with the same functional protein obtained by replacing, inserting or deleting one or more amino acids in the amino acid sequence shown in the (1).
  3. Use of a GSN7 gene and/or GSN7 protein expression and/or activity reduction, a GSN7 gene and/or GSN7 protein inhibitor or a biological material comprising said inhibitor for improving the appearance quality of plant kernels, characterized in that said GSN7 protein comprises any one of the amino acid sequences: (1) An amino acid sequence as shown in SEQ ID NO.3 or 6; (2) The amino acid sequence with the same functional protein obtained by replacing, inserting or deleting one or more amino acids in the amino acid sequence shown in the (1).
  4. The expression and/or activity of GSN7 gene and/or GSN7 protein is reduced, and the inhibition factor of GSN7 gene and/or GSN7 protein or the application of the biological material containing the inhibition factor in any one of the following (1) - (3): (1) Improving the grain shape of plant seeds; (2) Reducing the chalkiness rate of plant seeds; (3) The transparency of the plant seeds is improved; the GSN7 protein comprises any one of the following amino acid sequences: (1) An amino acid sequence as shown in SEQ ID NO.3 or 6; (2) The amino acid sequence with the same functional protein obtained by replacing, inserting or deleting one or more amino acids in the amino acid sequence shown in the (1).
  5. 5. The use according to any one of claims 1 to 4, wherein the gene encoding GSN7 protein comprises any one of the following nucleotide sequences: (1) A nucleotide sequence shown as SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.4 or SEQ ID NO. 5; (2) A complete complement of the nucleotide sequence as set forth in (1); (3) The nucleotide sequence shown in (1) can code a nucleotide sequence with the same functional protein obtained by replacing, deleting or inserting one or more nucleotides.
  6. 6. The use according to any one of claims 1 to 5, wherein the plant comprises rice.
  7. 7. The use according to any one of claims 1 to 6, wherein the biological material comprises a nucleic acid molecule, an expression cassette, a vector, a cell or a microorganism.
  8. 8. A method for improving the appearance quality of plant seeds is characterized by comprising the steps of reducing the expression and/or activity of GSN7 protein in plants; the GSN7 protein comprises any one of the following amino acid sequences: (1) An amino acid sequence as shown in SEQ ID NO.3 or 6; (2) The amino acid sequence with the same functional protein obtained by replacing, inserting or deleting one or more amino acids in the amino acid sequence shown in the (1).
  9. 9. The method of claim 8, wherein the method of reducing expression and/or activity of GSN7 protein in a plant comprises attenuating or knocking out a gene encoding GSN7 protein in the plant, or The promoter of the gene encoding the GSN7 protein is replaced with a weak promoter.
  10. 10. The method of claim 8 or 9, wherein the plant comprises rice.

Description

Application of GSN7 protein or coding gene thereof in regulating and controlling appearance quality of plant seeds Technical Field The invention relates to the technical field of plant breeding, in particular to application of GSN7 protein or a coding gene thereof in regulating and controlling appearance quality of plant seeds. Background Rice (Oryza sativa L.) is one of the major food crops in the world, rice is a shelled rice seed commonly known as rice, and nearly half of the population worldwide takes rice as the principal food. With the improvement of the living standard of people and the opening of the rice market, the requirements of people on the quality of rice appearance, cooking taste and the like are increasingly improved, and the task of improving the rice quality is more and more urgent in the international market. The appearance quality of rice is an important component of rice quality and is also one of important indexes for breeding high-quality rice varieties. The appearance quality of rice is mainly determined by the characteristics of grain shape, chalkiness, transparency and the like. The particulate form is generally expressed in terms of length, width and aspect ratio. Chalkiness is the lack of tight accumulation of starch and protein particles in the endosperm and can be measured in terms of chalkiness rate, chalkiness area and chalkiness, which are a grading indicator of appearance quality. The rice appearance quality related traits are complex, polygenic controlled quantitative traits, and studies indicate that grain shape traits are often closely related to chalky and transparent traits. By utilizing various genetic groups (such as DH group, F2 group, RIL group, CSSL group and the like) and mutants and the like, a group of QTL (Quantitative Trait Loci) and genes for controlling appearance quality related traits have been positioned and cloned by a person skilled in the art, and molecular basis and regulation mechanism for forming the appearance quality traits are analyzed, but the genes which can be directly applied in the breeding of high-quality rice still are lacking at present. Therefore, the method for accelerating the excavation of the key genes, excellent alleles and haplotypes for regulating the appearance quality of the rice and analyzing the genetic regulation network has important significance for rapidly improving the cultivation process of high-quality new varieties of the rice. Disclosure of Invention In order to solve the problems in the prior art, the invention provides application of GSN7 protein or a coding gene thereof in regulating and controlling the appearance quality of plant seeds. The invention uses the segregation population constructed by the rice chromosome single segment substitution material, adopts the map cloning method to determine a gene for controlling the appearance quality (grain shape, chalky and transparency) of rice, and names the gene as GSN7 gene. The expression level of the gene can be changed to realize the regulation of the appearance quality of rice. Based on the above, the invention provides the following technical scheme: In a first aspect, the invention provides application of GSN7 protein, or a coding gene thereof, or biological material containing the coding gene thereof in regulating and controlling the appearance quality of plant grains, wherein the GSN7 protein comprises any one of the following amino acid sequences (1) shown as SEQ ID NO.3 or 6, and (2) the amino acid sequence with the same functional protein obtained by replacing, inserting or deleting one or more amino acids in the amino acid sequence shown as (1). The GSN7 protein, or the coding gene thereof, or the biological material containing the coding gene thereof provided by the invention is applied to the regulation of the appearance quality of plant seeds, preferably, the regulation is negative regulation. More preferably, the appearance quality of the negative regulation plant seed grain comprises any one of (1) improving the grain shape of the plant seed grain, further preferably any one or more of improving the grain length, reducing the grain width and/or increasing the grain length-width ratio of the plant seed grain, (2) reducing the chalky rate of the plant seed grain and (3) improving the transparency of the plant seed grain. In the present invention, the grain length is the length of the grain, the grain width is the width of the grain, and the aspect ratio is the ratio of the grain length to the grain width. The GSN7 protein or the encoding gene thereof or the application of biological materials containing the encoding gene thereof in regulating the appearance quality of plant seeds, preferably, the encoding gene of the GSN7 protein comprises any one nucleotide sequence (1) shown as SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.4 or SEQ ID NO.5, (2) a complete complementary sequence of the nucleotide sequence shown as (1), and (3) a nucleotide sequence which is obt