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CN-121975841-A - Application of IRX15 protein and encoding gene thereof in promoting eggplant fruit coloring under weak light

CN121975841ACN 121975841 ACN121975841 ACN 121975841ACN-121975841-A

Abstract

The invention discloses an IRX15 protein and application of a coding gene thereof in promoting eggplant fruit coloring under weak light, and belongs to the technical field of genetic engineering. The invention discovers that IRX15 gene participates in the biosynthesis process of the anthocyanin of the eggplant and plays a positive regulation role in the process. The IRX15 gene is overexpressed in the photosensitive eggplant strain, so that the coloring of eggplant fruits under weak light can be promoted, the anthocyanin content in peel can be improved, and the method has important significance for the breeding of weak light-resistant eggplants.

Inventors

  • LI JING
  • XIN YU
  • YANG FENGJUAN
  • JI TUO

Assignees

  • 山东农业大学

Dates

Publication Date
20260505
Application Date
20260203

Claims (8)

  1. Use of irx15 protein in (1) or (2) as follows: (1) Promoting eggplant fruit coloration under weak light; (2) Improving anthocyanin content in eggplant peel; The IRX15 protein is a protein shown in the following (A1) or (A2): (A1) A protein with an amino acid sequence shown as SEQ ID NO. 1; (A2) A protein obtained by ligating the N-terminal and/or C-terminal of the protein defined in (A1) with a protein tag.
  2. Use of irx15 gene in at least one of the following (1) - (3): (1) Promoting eggplant fruit coloration under weak light; (2) Improving anthocyanin content in eggplant peel; (3) Cultivating a light-insensitive or light-semi-sensitive eggplant variety; the IRX15 gene is a nucleic acid molecule as shown in (i) or (ii) or (iii): (i) A nucleic acid molecule with a nucleotide sequence shown as SEQ ID NO. 2; (ii) A nucleic acid molecule other than (i) encoding the amino acid sequence shown in SEQ ID NO. 1; (iii) A nucleic acid molecule which has 90% or more identity to the nucleic acid molecule defined in (i) or (ii) and which encodes a protein functionally equivalent to the protein shown in SEQ ID NO. 3.
  3. 3. Use of a substance that modulates IRX15 gene expression or a substance that modulates IRX15 protein activity and/or content in at least one of the following (1) - (3): (1) Promoting eggplant fruit coloration under weak light; (2) Improving anthocyanin content in eggplant peel; (3) Cultivating light insensitive or light semi-sensitive eggplant variety.
  4. 4. Use according to claim 3, characterized in that the substance regulating IRX15 gene expression or the substance regulating IRX15 protein activity and/or content is any of the following: C1 An expression cassette comprising the IRX15 gene; c2 A recombinant vector containing the IRX15 gene, or a recombinant vector containing the expression cassette of C1); c3 A recombinant microorganism comprising the IRX15 gene, or a recombinant microorganism comprising the expression cassette of C1), or a recombinant microorganism comprising the recombinant vector of C2); c4 A transgenic plant cell line comprising the IRX15 gene, or a transgenic plant cell line comprising the expression cassette of C1); c5 A transgenic plant tissue comprising the IRX15 gene, or a transgenic plant tissue comprising the expression cassette of C1).
  5. 5. A method for promoting the coloration of eggplant fruits under weak light is characterized by comprising the step of over-expressing IRX15 genes in eggplant fruits.
  6. 6. The method according to claim 5, wherein the overexpression of IRX15 gene in eggplant fruit is achieved by: cloning IRX15 gene into plant expression vector to obtain recombinant vector, and transferring the recombinant vector into eggplant fruit.
  7. 7. The method according to claim 6, wherein the recombinant vector is transferred into eggplant fruits by transient injection, agroinfiltration or gene gun method.
  8. 8. A method for cultivating a light insensitive or light semi-sensitive eggplant variety, comprising the steps of: And (3) exogenous transfer of IRX15 gene into the photosensitive eggplant strain to enable the IRX15 gene to be over-expressed, and cultivation to obtain the light-insensitive or light-semi-sensitive eggplant variety.

Description

Application of IRX15 protein and encoding gene thereof in promoting eggplant fruit coloring under weak light Technical Field The invention relates to the technical field of genetic engineering, in particular to application of IRX15 protein and a coding gene thereof in promoting eggplant fruit coloring under weak light. Background Eggplant (Solanum melongena l.) is a widely cultivated plant of the solanaceae family worldwide, and its nutrient-rich purple fruits have extremely high anthocyanin content. Anthocyanin is a water-soluble flavonoid compound, can enable plant tissues to present bright red and blue color, and has the key physiological functions of ultraviolet light protection, pathogen invasion resistance, oxidization resistance and the like (Grotewold, 2006;Kuljarusnontet al, 2024). The environment factors are closely related to anthocyanin synthesis, and the eggplant material is researched into three types according to the relationship between the color and light of eggplant peel, namely a non-photosensitive type in which anthocyanin synthesis in eggplant peel is not affected under dark conditions, a photosensitive type in which anthocyanin synthesis is not affected under light-shielding environments, and a light semi-sensitive type in which little anthocyanin synthesis is still contained in eggplant peel under dark conditions. In the production process, weak light conditions such as plant density, greenhouse cultivation and the like often lead to poor coloring of light-sensitive eggplant fruits, and seriously influence the quality of the eggplant fruits. Therefore, the digging of the regulatory genes involved in the synthesis of the anthocyanin of the light-insensitive or light-semi-sensitive eggplant under the dark condition has important significance for promoting the coloring of fruits under weak light and the breeding of the weak light-resistant eggplant. Disclosure of Invention Aiming at the prior art, the invention aims to provide an application of IRX15 protein and a coding gene thereof in promoting eggplant fruit coloring under weak light. In order to achieve the above purpose, the invention adopts the following technical scheme: In a first aspect of the invention there is provided the use of an IRX15 protein in (1) or (2) as follows: (1) Promoting eggplant fruit coloration under weak light; (2) Improving anthocyanin content in eggplant peel; The IRX15 protein is a protein shown in the following (A1) or (A2): (A1) A protein with an amino acid sequence shown as SEQ ID NO. 1; (A2) A protein obtained by ligating the N-terminal and/or C-terminal of the protein defined in (A1) with a protein tag. Among the above proteins, the protein tag (protein-tag) refers to a polypeptide or protein that is fusion expressed together with a target protein by using a DNA in vitro recombination technique, so as to facilitate the expression, detection, tracing and/or purification of the target protein. The protein tag may be a Flag tag, his tag, MBP tag, HA tag, myc tag, GST tag, and/or SUMO tag, etc. In a second aspect of the invention there is provided the use of the IRX15 gene in at least one of the following (1) - (3): (1) Promoting eggplant fruit coloration under weak light; (2) Improving anthocyanin content in eggplant peel; (3) Cultivating a light-insensitive or light-semi-sensitive eggplant variety; the IRX15 gene is a nucleic acid molecule as shown in (i) or (ii) or (iii): (i) A nucleic acid molecule with a nucleotide sequence shown as SEQ ID NO. 2; (ii) A nucleic acid molecule other than (i) encoding the amino acid sequence shown in SEQ ID NO. 1; (iii) A nucleic acid molecule which has 90% or more identity to the nucleic acid molecule defined in (i) or (ii) and which encodes a protein functionally equivalent to the protein shown in SEQ ID NO. 3. In the above applications, the nucleic acid molecule may be DNA, such as cDNA, genomic DNA or recombinant DNA, or RNA, such as gRNA or mRNA. The term "identity" as used herein refers to sequence similarity to a native nucleic acid sequence. Identity can be assessed using computer software, e.g., using the BLAST algorithm （Altschulet al.1990. Journal of Molecular Biology 215:403-410; Karlin and Altschul. 1993. Proceedings of the National Academy of Sciences 90:5873-5877）. In the above nucleic acid molecules, the 90% or more identity may be at least 90%, 92%, 93%, 95%, 96%, 98% or 99% identity. The invention discovers for the first time that IRX15 gene presents an obvious light response mode in eggplant peel, participates in the biosynthesis process of eggplant anthocyanin, plays a positive regulation role in the process, and promotes eggplant fruit coloring under the condition of weak light. The IRX15 gene is over-expressed in the eggplant, so that the cultivation of the light-insensitive or light-semi-sensitive eggplant variety is facilitated. In a third aspect of the invention there is provided the use of a substance that modulates IRX15 gene expression or a s