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CN-121975843-A - Application of MaFLA gene in improving banana vascular wilt resistance

CN121975843ACN 121975843 ACN121975843 ACN 121975843ACN-121975843-A

Abstract

The invention discloses an application of MaFLA gene in improving banana vascular wilt resistance. The MaFLA gene is utilized to construct a strain which is used for over-expressing the MaFLA gene, the strain is transformed into banana embryogenic suspension system cells to obtain a banana strain which is used for over-expressing the MaFLA gene, and banana fusarium wilt bacteria are further inoculated to the strain, and the result shows that the leaf fusarium wilt yellowing degree and the bulb infection pathogenic bacteria degree of the banana strain which is used for over-expressing the MaFLA gene are lower than those of wild plants, the morbidity of the leaf and the bulb is 70.63-80.83% and 90.00-95.33%, and the disease index of the leaf and the bulb is 43.17-50.56% and 47.67-51.18%. The over-expression MaFLA is shown to obviously improve the resistance of the plant to banana vascular wilt, and provides theoretical and practical basis for cultivating banana varieties resistant to banana vascular wilt.

Inventors

  • XU CHUNXIANG
  • ZHAN HUILING
  • Dai Longyu

Assignees

  • 华南农业大学

Dates

Publication Date
20260505
Application Date
20260209

Claims (10)

  1. The application of the MaFLA29 gene and the biological material containing MaFLA gene in improving banana vascular wilt resistance is characterized in that the amino acid sequence coded by the MaFLA gene is shown as SEQ ID No. 2.
  2. The application of the MaFLA29 gene and the biological material containing MaFLA gene in improving the tolerance of banana to banana fusarium wilt is characterized in that the amino acid sequence coded by the MaFLA gene is shown as SEQ ID No. 2.
  3. 3. The use according to claim 2, wherein the banana vascular wilt is Foc.
  4. The application of the MaFLA29 gene and the biological material containing MaFLA gene in creating banana varieties resistant to banana vascular wilt is characterized in that the amino acid sequence of MaFLA gene codes is shown as SEQ ID No. 2.
  5. 5. The use according to any one of claims 1 to 4, wherein the biological material comprises an expression cassette, an expression vector or an expression strain comprising MaFLA genes.
  6. 6. The application of the agent for improving MaFLA gene expression in improving banana vascular wilt resistance, banana vascular wilt resistance or creating banana vascular wilt resistance, is characterized in that the amino acid sequence of MaFLA gene coding is shown as SEQ ID No. 2.
  7. 7. A method for creating banana varieties resistant to banana vascular wilt is characterized in that MaFLA genes are overexpressed in banana strains through transgenic means, and the amino acid sequence coded by MaFLA genes is shown as SEQ ID No. 2.
  8. 8. The method of claim 7, wherein the overexpressing MaFLA gene in banana strains is overexpressing MaFLA gene in banana embryonal suspension cells.
  9. 9. The method of claim 8, wherein the transgenic means is agrobacterium transformation.
  10. 10. The use according to any one of claims 1 to 4 or the method according to any one of claims 7 to 8, wherein the nucleotide sequence of the MaFLA gene is shown as SEQ ID No. 1.

Description

Application of MaFLA gene in improving banana vascular wilt resistance Technical Field The invention relates to the technical field of genetic engineering, in particular to application of MaFLA gene in improving banana wilt resistance. Background The plant cell wall is mainly composed of cellulose, hemicellulose, pectin, lignin and about 10% structural proteins. The structural proteins mainly comprise the major classes of extensin, arabinogalactan protein (Arabinogalactan proteins, AGPs), glycine-rich protein, proline-rich protein and lectin 5, all belonging to the hydroxyproline-rich glycoprotein family (Showalter et al., 2010). The Fasciclin-like arabinogalactan protein (Fasciclin-like arabinogalactan proteins, FLAs) belongs to the family of AGPs with the highest degree of glycosylation among cell wall structural proteins, and contains, in addition to the AG glycosylation region, a Fasciclin domain with extremely complex functions (Ma et al, 2023). The former can crosslink with pectin, and the latter acts as an intercellular communication, which can interact with cell wall polysaccharides, cell wall related kinases, etc. to regulate cell wall biosynthesis (Ma et al 2022), such as cotton GhFLA9 together with GhCOBL9 to regulate cell wall development by regulating cellulose synthesis (He et al 2025). Most FLAs proteins localize to the plasma membrane/cell wall/cell surface and possess an N-terminal signal peptide, and most FLAs also possess a C-terminal glycosyl phosphatidylinositol plasma membrane anchor (Ma et al 2023). Therefore, FLAs of the plant has the function of sensing stress signals, can also interact with other cell wall components, can regulate and control cell wall biosynthesis and the like through interaction with cell wall related kinases, and plays a vital role in cell wall remodeling. Plant FLAs affects the growth and development of the plant. In recent years, with the development of molecular biology techniques, the function of plant FLAs genes during growth and development has been gradually verified. If AtFLA is capable of regulating the root growth of Arabidopsis thaliana (Xue AND SEIFERT, 2015). Arabidopsis AtFLA/12 is involved in fiber development (Liu et al 2020). Cotton GhAGP can increase the number of epidermal hairs in leaves and stems of arabidopsis thaliana (Wei et al 2025). FLAs has been shown to be closely related to pollen development in a variety of plants such as rice (Zhou et al 2022). Plant FLAs has stress-resistant function. For example, shi et al (2003) reported that Arabidopsis salt hypersensitive mutant sos5 (AtFLA mutant) had a higher sensitivity to salt than the wild type, indicating that the AtFLA gene was able to increase plant salt tolerance, and more recently, chao et al (2025) also demonstrated that mutations in rice OsFLA8 increased its sensitivity to salt, while lignin deposition was reduced in the root cortex after OsFLA knockouts. In contrast, niu et al (2024) found that over-expression of TIANYUANHOUXIA FLA11 increased the cellulose content of Populus alba but decreased lignin content and salt tolerance. AtFLA11/12 can influence the mechanical stress resistance of the over-expressed plants by regulating the balance between cellulose and lignin biosynthesis (Ma et al, 2022), the drought resistance of tobacco can be improved by over-expressing potato StFLA4 (Nie et et al, 2024), the resistance of arabidopsis to clubroot can be negatively regulated by homologous gene AtFLA7 of brassica napus BnFLA39 in arabidopsis (Jia et al, 2023), and the frost resistance of arabidopsis can be improved by over-expressing banana MaFLA27 proved in earlier researches (Liu et al, 2025). The banana vascular wilt is a soil-borne vascular bundle disease, generates great harm to the banana industry worldwide, restricts the development of the banana industry, and has no effective control method at present. Breeding and culturing of anti-fusarium wilt varieties are currently considered as the most direct and effective method for controlling fusarium wilt. Cell walls are the first physical barrier to environmental threat for plants, and play a key role in the disease resistance of plants. Although FLAs has been reported in the prior art to play an important role in plant growth and development and abiotic stress, research and report of FLAs on plant disease resistance have not been reported so far because of high glycosylation degree of FLAs, complex structure and function, limited early technology and the like. Disclosure of Invention The invention aims to overcome the defects and the shortcomings in the prior art and provide MaFLA gene and application of a biological material containing MaFLA gene in improving banana resistance to banana vascular wilt. The second object of the invention is to provide MaFLA gene and the application of the biological material containing MaFLA gene in improving the tolerance of banana to banana fusarium wilt. The third object of the present invention i