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CN-121975847-A - Application of TaERF gene in synergetic histone modification in participating in nitrogen-mediated root system development

CN121975847ACN 121975847 ACN121975847 ACN 121975847ACN-121975847-A

Abstract

The invention discloses an application of TaERF genes in synergetic histone modification in participating in nitrogen-mediated root system development, wherein homologous genes of three subgenomic groups of the TaERF genes respectively correspond to GenBank numbers CM022223.1, CM022224.1 and CM022225.1 in NCBI. According to the invention, wheat KN199 is directionally edited by using a CRISPR-Cas9 technology, and a TaERF functional deletion mutant plant is obtained. Protein interaction experiments demonstrated that TaERF was able to interact with subunit SWN of PRC 2. The high-low nitrogen hydroponic experiment result shows that TaERF gene mutation significantly promotes root system development under high nitrogen condition, and under low nitrogen condition, mutant root system development keeps similar level with wild type, shows the ability of balancing root system development under different nitrogen supply conditions, and reveals the function of TaERF gene in nitrogen response mediated root system development regulation. That is to say, taERF gene mutation can promote root system development, thereby providing gene resources for crop nitrogen efficient breeding.

Inventors

  • XIAO JUN
  • Dai Qirui
  • LIN XUELEI
  • WANG XIAOHAN
  • ZHANG ZHAOHENG
  • ZHANG HAO

Assignees

  • 中国科学院遗传与发育生物学研究所

Dates

Publication Date
20260505
Application Date
20260303

Claims (8)

  1. The application of the TaERF9 gene in the synergistic histone modification to participate in nitrogen-mediated plant root system development is characterized in that nucleotide sequences of homologous genes TaERF-5A, taERF9-5B, taERF9-5D of three subgenomic groups of the TaERF gene are respectively shown as SEQ ID NO. 1-3, amino acid sequences of corresponding TaERF9 proteins are respectively shown as SEQ ID NO. 4-6, and the histone modification is histone modification H3K27me3.
  2. 2. The use according to claim 1, wherein the plant is wheat.
  3. 3. The use according to claim 1, wherein TaERF function-deleted mutant plants are obtained by constructing TaERF CRISPR vectors, wherein the mutant plants have stronger root system development ability than the wild type under high nitrogen conditions, and the mutant plants have reduced response to low nitrogen under low nitrogen conditions, and the root system development level is not different from that of the wild type.
  4. 4. A plant breeding method is characterized in that a plant with root system development capacity stronger than that of a target plant is obtained by inhibiting the expression of TaERF genes in the target plant, and nucleotide sequences of homologous genes TaERF9-5A, taERF9-5B, taERF9-5D of three subgenomic groups of TaERF genes are respectively shown as SEQ ID NO. 1-3.
  5. 5. The method of plant breeding according to claim 4, wherein the plant of interest is wheat.
  6. 6. The method of plant breeding according to claim 4, wherein the expression of TaERF gene in the plant of interest is inhibited by knocking out TaERF gene.
  7. 7. The plant breeding method according to claim 6, wherein the knocked-out TaERF gene adopts CRISPR/Cas9 technology to target and edit TaERF gene, and TaERF functional deletion mutant is obtained.
  8. 8. A method for improving the root system development capability of wheat under the high nitrogen condition is characterized by knocking out TaERF genes in the wheat.

Description

Application of TaERF gene in synergetic histone modification in participating in nitrogen-mediated root system development Technical Field The invention belongs to the technical field of biology, and particularly relates to application of TaERF genes in synergetic histone modification in nitrogen-mediated root system development. Background Nitrogen (Nitrogen) is an important nutrient substance and participates in various links of crop growth and development. Nitrogen fertilizers are critical in agricultural production, so a large amount of nitrogen fertilizer is put into use worldwide each year. As the amount of nitrogen fertilizer used continues to increase, environmental pollution problems such as water pollution and soil acidification are gradually developed, so that the excessive use of nitrogen fertilizer is reduced, and the improvement of nitrogen utilization efficiency (Nitrogen Use Efficiency, NUE) is urgent. Nitrate is not only a nutrient substance but also a key signal molecule, a plurality of development processes of plants can be regulated and controlled by nitrate signals, such as releasing seed dormancy, promoting leaf growth, inducing flowering, affecting root system development and the like, the nitrate signals participate in regulating and controlling the processes of rice tillering, rice ear structure, crop root system development and the like through different regulating and controlling modules, wherein the influence of nitrate on the root system is particularly important, nitrate is absorbed by the plants mainly through the root system, the absorption efficiency of the plants on nitrogen is determined to a great extent by the structure of the root system, and regulating and controlling channels of nitrate on root system development are gradually discovered. Nitrate can increase gibberellin level in plants, promote degradation of growth inhibitory protein DELLA, activate cell proliferation, and promote root and bud growth. AtANR1 in Arabidopsis encodes a MADS-box transcription factor involved in promoting lateral root elongation under localized nitrate treatment. AtAFB3 (Auxin SIGNALING F-BOX 3) is induced by intracellular nitrate increase and regulates downstream genes such as NAC3/OBP4, which are involved in regulation of lateral root initiation and main root development. Epigenetic regulation plays a key role in controlling plant response to environmental signals. In monocotyledonous plants, nitrogen uptake is regulated by changes in chromatin rearrangement. CHB101 encodes a subunit of ATP dependent chromatin remodeling complexes, SWI/SNF complexes. In maize ZmCHB a 101 has been found to regulate nitrate transporter gene expression including zmnrt2.1 and zmnrt2.2 by responding to nitrate supply. In wheat, taWFZP regulates and controls the expression of lateral root development genes TaCRL1 and TaRHD6 through mediating chromatin remodelling factors TaSYD to influence root system development, and simultaneously can promote the expression of genes such as TaNRT2.1, taNRT2.3 and the like to improve nitrogen absorption efficiency so as to improve nitrogen utilization efficiency. In addition, histone modification regulates and controls the adaptive development strategy of the root system under low nitrogen among different varieties of wheat. In KN9204, the increase in H3K27ac and the decrease in H3K27me3 promote root growth, while in J411, the increase in H3K27ac and the decrease in H3K27me3 promote a nitrate transport system. Changing the modification state of H3K27me3 can change the strategy of the adaptive development of low nitrogen of the root system. In view of the adverse effect of nitrogen fertilizer on ecological environment, the key genes which mediate histone modification and participate in root system development of nitrogen response are mined through multiple groups of chemical data, and the transgenic material is created by utilizing the transgenic technology and is used for deeply researching the gene function and the action mechanism of the genetic material which are used for regulating and controlling root system development by mining the nitrogen signal of wheat, so that a solid research foundation is provided for creating and cultivating nitrogen-efficient wheat varieties. Disclosure of Invention The invention aims to provide an application of TaERF gene in synergetic histone modification in participating in nitrogen-mediated root system development. In order to achieve the above purpose, the technical scheme adopted by the invention is summarized as follows: homologous genes of three subgenomic groups of TaERF genes adopted by the invention respectively correspond to GenBank numbers CM022223.1, CM022224.1 and CM022225.1 in NCBI. The length of messenger RNA (mRNA) of TaERF-5A gene is 1,179 bp, the length of coding sequence of TaERF9-5A gene is 726 bp, the nucleotide sequence is shown as SEQ ID NO.1, including 241 amino acids, and the amino acid sequence is shown as SEQ ID