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CN-121975848-A - Application of rice OsCBSX gene in improving resistance of rice to ustilaginoidea virens

CN121975848ACN 121975848 ACN121975848 ACN 121975848ACN-121975848-A

Abstract

The invention discloses application of a rice OsCBSX gene in improving the resistance of rice to ustilaginoidea virens, and belongs to the technical field of genetic engineering. The nucleotide sequence of the CDS region of the rice OsCBSX gene is shown as SEQ ID NO. 2. According to the research of the invention, osCBSX genes are related to the resistance of rice to false smut, osCBSX gene mutation reduces the defending capability of rice to false smut, and over-expression OsCBSX genes obviously improves the disease resistance of rice to false smut. Therefore, the rice OsCBSX gene can be used for improving the resistance of rice to ustilaginoidea virens and diseases caused by the rice.

Inventors

  • KOU YANJUN
  • WEN HUI
  • LU YEZI
  • QIU JIEHUA
  • SHI HUANBIN

Assignees

  • 中国水稻研究所

Dates

Publication Date
20260505
Application Date
20260305

Claims (10)

  1. 1. Use of the rice OsCBSX gene in (1) or (2) below: (1) The application in improving the resistance of rice to ustilaginoidea virens; (2) Application in cultivating rice with anti-ustilaginoidea virens; Wherein the nucleotide sequence of the CDS region of the rice OsCBSX gene is shown as SEQ ID NO. 2.
  2. 2. The application of the protein coded by the rice OsCBSX gene in the following (1) or (2): (1) The application in improving the resistance of rice to ustilaginoidea virens; (2) Application in cultivating rice with anti-ustilaginoidea virens; Wherein the nucleotide sequence of the CDS region of the rice OsCBSX gene is shown as SEQ ID NO. 2.
  3. 3. Use of a recombinant vector comprising a rice OsCBSX gene in the following (1) or (2): (1) The application in improving the resistance of rice to ustilaginoidea virens; (2) Application in cultivating rice with anti-ustilaginoidea virens; The recombinant vector is obtained by introducing a rice OsCBSX gene into an expression vector, and the nucleotide sequence of a CDS region of the rice OsCBSX gene is shown as SEQ ID NO. 2.
  4. 4. The application of the recombinant engineering bacteria containing rice OsCBSX gene in the following (1) or (2): (1) The application in improving the resistance of rice to ustilaginoidea virens; (2) Application in cultivating rice with anti-ustilaginoidea virens; The recombinant engineering bacteria are obtained by transferring a recombinant vector containing rice OsCBSX genes into agrobacterium, the recombinant vector is obtained by introducing rice OsCBSX genes into an expression vector, and the nucleotide sequence of a CDS region of the rice OsCBSX genes is shown as SEQ ID NO. 2.
  5. 5. The use according to any one of claims 1 to 4, wherein the rice plant is raised against ustilaginoidea virens by overexpressing the rice OsCBSX gene in the rice plant.
  6. 6. The use according to any one of claims 1 to 4, wherein rice plants which are high in expression of the rice OsCBSX gene are selected and maintained as anti-ustilaginoidea virens rice by introducing the rice OsCBSX gene into rice plants.
  7. 7. A method for improving the resistance of rice to ustilaginoidea virens is characterized by comprising the step of over-expressing a rice OsCBSX gene in the rice to improve the resistance of the rice to ustilaginoidea virens, wherein the nucleotide sequence of a CDS region of the rice OsCBSX gene is shown as SEQ ID NO. 2.
  8. 8. The method according to claim 7, wherein the over-expression method comprises cloning the rice OsCBSX gene into an expression vector, constructing a recombinant vector, introducing the recombinant vector into rice cells through callus transformation, and culturing the rice cells into plants.
  9. 9. A method for cultivating rice resistant to ustilaginoidea virens is characterized by comprising the steps of over-expressing rice OsCBSX genes in rice, improving the expression level of the rice OsCBSX genes, and obtaining rice with high expression of the rice OsCBSX genes, namely rice resistant to ustilaginoidea virens, wherein the nucleotide sequence of a CDS region of the rice OsCBSX genes is shown as SEQ ID No. 2.
  10. 10. The method according to claim 9, wherein the over-expression method comprises cloning the rice OsCBSX gene into an expression vector, constructing a recombinant vector, introducing the recombinant vector into rice cells through callus transformation, and culturing the rice cells into plants.

Description

Application of rice OsCBSX gene in improving resistance of rice to ustilaginoidea virens Technical Field The invention relates to the technical field of genetic engineering, in particular to application of a rice OsCBSX gene in improving the resistance of rice to ustilaginoidea virens. Background Rice is used as an important grain crop, is critical to the grain safety of developing countries because of high nutritive value and strong environmental adaptability, but the growth process is often affected by various diseases. False smut is a fungal disease caused by false smut (Ustilaginoidea virens), and is now another important disease which severely restricts the high quality and high yield of rice after rice blast and bacterial leaf blight. The typical symptoms of the disease are that false smut is formed and various toxins are polluted, so that the false smut not only can seriously reduce the yield of rice and reduce the quality of rice, but also can endanger the safety of people and animals. At present, although chemical prevention and control are still the main means for preventing and controlling the current false smut, the chemical prevention and control still faces multiple technical dilemma that on one hand, the critical period of false smut drug application is difficult to accurately grasp, and practical difficulties are brought to field prevention and control, and on the other hand, the continuous use of chemical pesticides easily causes farmland environment pollution, and part of medicaments have single action sites, and the problem of drug resistance is extremely easy to induce after long-term continuous application. In order to meet the development requirement of green control of modern agriculture, the application of the resistance gene to biological breeding and agricultural production is a green and efficient strategy, which can effectively ensure the yield and quality of crops and has important significance for promoting sustainable development of agriculture. With the increasing depth of research on rice-ustilaginoidea virens interaction, an important entry point is provided for clarifying the pathogenic mechanism of plant pathogenic fungi. The deepening of the research is not only helpful for revealing the pathogenic rule of pathogenic bacteria, but also provides theoretical guidance and technical support for scientific prevention and control of false smut and molecular breeding for resisting false smut. Some genes of rice themselves may act against ustilaginoidea virens. For example, the invention with publication number CN111676228A discloses a false smut resistant gene OsRFS gene, and the invention can change the resistance of rice to false smut by changing the expression abundance of OsRFS gene through a genetic engineering technology. Through research on biological functions of OsRFS genes, it is proved that over-expression of OsRFS genes in rice can enable rice to show resistance enhancement capability to ustilaginoidea virens, and disease resistance of rice to ustilaginoidea virens is affected by adjusting OsRFS gene expression level. OsRFS2 gene can be used for cultivating rice varieties resistant to false smut. Therefore, the excavation of new genes of rice with anti-ustilaginoidea virens has important significance. Disclosure of Invention The invention aims to provide application of a rice OsCBSX gene in improving the resistance of rice to ustilaginoidea virens, so as to solve the problems in the prior art, and the resistance of the rice to ustilaginoidea virens can be obviously improved by over-expressing the OsCBSX gene in the rice. In order to achieve the above object, the present invention provides the following solutions: the invention provides application of rice OsCBSX gene in the following (1) or (2): (1) The application in improving the resistance of rice to ustilaginoidea virens; (2) Application in cultivating rice with anti-ustilaginoidea virens; Wherein the nucleotide sequence of the CDS region of the rice OsCBSX gene is shown as SEQ ID NO. 2. The invention also provides application of the protein coded by the rice OsCBSX gene in the following (1) or (2): (1) The application in improving the resistance of rice to ustilaginoidea virens; (2) Application in cultivating rice with anti-ustilaginoidea virens; Wherein the nucleotide sequence of the CDS region of the rice OsCBSX gene is shown as SEQ ID NO. 2. The invention also provides application of the recombinant vector containing the rice OsCBSX gene in the following (1) or (2): (1) The application in improving the resistance of rice to ustilaginoidea virens; (2) Application in cultivating rice with anti-ustilaginoidea virens; The recombinant vector is obtained by introducing a rice OsCBSX gene into an expression vector, and the nucleotide sequence of a CDS region of the rice OsCBSX gene is shown as SEQ ID NO. 2. The invention also provides application of the recombinant engineering bacteria containing the rice OsCBSX ge