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CN-121975850-A - Application of OsNAC15 gene in improving resistance of rice to rhizoctonia solani

CN121975850ACN 121975850 ACN121975850 ACN 121975850ACN-121975850-A

Abstract

The invention provides an application of an OsNAC15 gene in improving the resistance of rice rhizoctonia solani, belonging to the technical field of biology. The invention provides an application of an agent for inhibiting OsNAC15 gene expression in improving rice rhizoctonia solani resistance or improving rice sheath blight resistance, firstly provides an OsNAC15 negative regulation on rice rhizoctonia solani resistance, supplements the function of OsNAC15 in rice and rhizoctonia solani interaction and an NAC transcription factor regulation PR gene disease resistance path, and provides a new thought for rice-rhizoctonia solani interaction mechanism research.

Inventors

  • WANG YAN
  • WEI SONGHONG
  • WANG YIMING
  • WANG MENGYU

Assignees

  • 沈阳农业大学

Dates

Publication Date
20260505
Application Date
20260316

Claims (10)

  1. 1. Application of an agent for inhibiting OsNAC15 gene expression in improving resistance of rice to Rhizoctonia solani.
  2. 2. Application of an agent for inhibiting OsNAC15 gene expression in improving rice sheath blight resistance.
  3. 3. The use of claim 1 or 2, wherein the inhibition comprises knockout, silencing or knockdown.
  4. 4. The use according to claim 1 or 2, wherein the agent comprises an sgRNA having the nucleotide sequence shown in SEQ ID No. 1.
  5. 5. A recombinant expression vector comprising an interfering nucleic acid molecule that targets the coding region of the OsNAC15 gene.
  6. 6. The use of the recombinant expression vector of claim 5 for increasing resistance to Rhizoctonia solani in rice or for increasing banded sclerotial blight resistance in rice.
  7. 7. The sgRNA targeting the OsNAC15 gene of rice is characterized in that the nucleotide sequence of the sgRNA is shown as SEQ ID NO. 1.
  8. 8. A method for improving resistance of rice to rhizoctonia solani, which is characterized by inhibiting expression of an OsNAC15 gene in rice.
  9. 9. The method of claim 8, wherein the inhibiting comprises knocking out, silencing, or knocking down, wherein the knocking out comprises a CRISPR/Cas9 method, and wherein the nucleotide sequence of the sgRNA used in the CRISPR/Cas9 method is set forth in SEQ ID No. 1.
  10. 10. A method for screening banded sclerotial blight-resistant rice is characterized in that the expression level of OsNAC15 genes in rice samples is detected, wherein the lower the expression level is, the higher the banded sclerotial blight-resistant level of the rice samples to be detected is, and the higher the expression level is, the lower the banded sclerotial blight-resistant level of the rice samples to be detected is.

Description

Application of OsNAC15 gene in improving resistance of rice to rhizoctonia solani Technical Field The invention belongs to the technical field of biology, and particularly relates to application of an OsNAC15 gene in improving resistance of rice rhizoctonia solani. Background Rhizoctonia solani-induced rice sheath blight disease is one of the most serious rice diseases in the world. The incidence of banded sclerotial blight in the field has increased dramatically over the last few decades, mainly due to the application of high-dose nitrogen fertilizer and the large-scale planting of semi-dwarf tall product species. At present, the control of rice sheath blight is highly dependent on cultivation measures and chemical bactericides, but due to the high genetic variability of rhizoctonia solani, the broad host affinity and the ability of pathogens to survive from one crop season to the next by forming dormant sclerotium, the difficulty in controlling pathogens is increased. Therefore, the cultivation of disease-resistant varieties is considered to be the most economical and effective way and is the most friendly to the ecological environment. However, there is currently no rice variety in the field that is highly resistant or fully immunized. In recent years, more and more disease-resistant genes are identified, and many researches have shown that PR genes play important roles in regulating the disease-resistant function of rice. The family of transcription factors NAC, WRKY, C2H2, etc. play a key role in plant life activities. Wherein NAC transcription factor is one of the largest family of transcription factors in plants. NAC transcription factors are used as core regulatory factors in plants, the functions of the NAC transcription factors are highly diverse, and the NAC transcription factors are widely involved in the growth and development regulation of secondary cell wall development, seed germination, root system development, leaf senescence, flower organ formation, fruit maturation and the like of plants, and respond to biotic stress and abiotic stress. OsNAC15 is a rice NAC family transcription factor, the prior report discloses that OsNAC15 is related to abiotic stress of plants, the related technical content of interaction of OsNAC15 with pathogenic bacteria is not disclosed, and in the prior rice banded sclerotial blight resistance research, the reported NAC family members are all other NAC genes, and no report related to OsNAC15 is seen. Disclosure of Invention Therefore, the invention aims to provide the application of the agent for inhibiting the gene expression of the OsNAC15 in improving the resistance of rice to Rhizoctonia solani, and discloses the function of the OsNAC15 in the interaction of rice and Rhizoctonia solani, and the disease resistance path of the PR gene regulated by the NAC transcription factor is supplemented. In order to achieve the above object, the present invention provides the following technical solutions: The invention provides an application of an agent for inhibiting OsNAC15 gene expression in improving resistance of rice rhizoctonia solani. The invention also provides application of the agent for inhibiting OsNAC15 gene expression in improving rice sheath blight resistance. Preferably, the inhibition comprises knockout, silencing or knockdown. Preferably, the reagent comprises sgRNA, and the nucleotide sequence of the sgRNA is shown as SEQ ID NO. 1. The invention also provides a recombinant expression vector, which comprises an interference nucleic acid molecule targeting the coding region of the OsNAC15 gene. The invention also provides application of the recombinant expression vector in improving the resistance of rice rhizoctonia solani or improving the banded sclerotial blight resistance of rice. The invention also provides a sgRNA of the targeted rice OsNAC15 gene, and the nucleotide sequence of the sgRNA is shown as SEQ ID NO. 1. The invention also provides a method for improving the resistance of the rice to the rhizoctonia solani, and inhibiting the expression of the OsNAC15 gene in the rice. Preferably, the inhibition comprises knockout, silencing or knockdown, the knockdown method comprises a CRISPR/Cas9 method, and the nucleotide sequence of the sgRNA used in the CRISPR/Cas9 method is shown as SEQ ID NO. 1. The invention also provides a method for screening the banded sclerotial blight-resistant rice, which is used for detecting the expression quantity of the OsNAC15 gene in the rice sample, wherein the lower the expression quantity is, the higher the banded sclerotial blight-resistant level of the rice sample to be detected is, and the higher the expression quantity is, the lower the banded sclerotial blight-resistant level of the rice sample to be detected is. The invention has the beneficial effects that: The invention provides the OsNAC15 for negatively regulating the resistance of rice to rhizoctonia solani for the first time, supplements the function