CN-121975851-A - Application of MdCPP gene in regulation and control of apple low-temperature stress

Abstract

The invention provides an application of MdCPP gene in regulating and controlling low-temperature stress of apples, wherein MdCPP gene in apple callus is over-expressed to reduce low-temperature stress tolerance of apple callus, or/and MdCPP gene in apple callus is knocked down to enhance low-temperature stress tolerance of apple callus, and fresh weight of apple callus knocked down with MdCPP gene is higher than that of wild apple callus under low-temperature stress. The MdCPP gene provides a new view for screening and innovation of apple cold-resistant germplasm resources, and provides a key functional gene target and molecular design basis for realizing accurate and efficient breeding of apple cold-resistant characters.

Inventors

• DONG HAIQIANG
• LIU SHIYU
• JI SHUYUN
• KANG FUREN
• WANG YUFAN
• WU ZHENZHONG
• LIANG FEIHONG
• GAO WEI
• LI JUN
• LIU YANLI
• PAN GE

Assignees

• 榆林学院

Dates

Publication Date

20260505

Application Date

20260318

Claims (4)

1. 1. The application of MdCPP gene in regulating and controlling apple low temperature stress is characterized in that the nucleotide sequence of the MdCPP gene coding region is shown as SEQ ID No. 1, and the amino acid sequence of the MdCPP gene coding region is shown as SEQ ID No. 2; by overexpressing MdCPP gene in apple callus, for reducing low temperature stress tolerance of the apple callus; or/and knocking down MdCPP genes in apple callus for enhancing low temperature stress tolerance of the apple callus.
2. 2. Use of MdCPP gene according to claim 1 for regulating low temperature stress in apples, wherein when the MdCPP gene in apple callus is overexpressed, the fresh weight of apple callus is lower than that of wild-type apple callus under low temperature stress.
3. 3. Use of MdCPP gene according to claim 1 for regulating low temperature stress in apples, wherein when MdCPP gene in apple callus is knocked down, the fresh weight of apple callus is higher than that of wild type apple callus under low temperature stress.
4. 4. The use of MdCPP gene according to claim 1 for controlling apple low temperature stress, wherein the temperature of said low temperature stress is 4 ℃.

Description

Application of MdCPP gene in regulation and control of apple low-temperature stress Technical Field The invention belongs to the technical field of plant genetic engineering, and particularly relates to application of MdCPP gene in regulation and control of apple low-temperature stress. Background Apples are one of the most important fruits in the world, and are rich in nutrition and have higher economic value. Low temperature is an abiotic stress that affects plant growth. Low temperature stress, including cold and freeze injury, develops when the ambient temperature continues to be below the optimal temperature for plant growth. Cold injury refers to injury to plants at low temperature of zero or above, and no ice is formed in cells, but physiological disorder can be caused to warm-like plants, and injury or death of the plants can be caused, and cold injury refers to phenomenon that cells are damaged or even dead at low temperature of below zero. In recent years, with global climate change, extreme weather frequently occurs, and low-temperature damage caused by winter freezing injury and spring reverse and spring cold seriously affects the yield and quality of apples. Under low temperature stress, the plant is metabolized and growth is inhibited, and the plant is reduced in yield and even dead when serious. Disclosure of Invention Aiming at the defects of the prior art, the invention provides the application of MdCPP gene in regulating and controlling the low-temperature stress of apples, and the MdCPP gene in the callus of apples is knocked down, so that the low-temperature stress tolerance of the callus of apples can be effectively enhanced, and potential effective candidate genes are provided for fruit tree anti-reversion gene breeding, thereby realizing sustainable development of apple industry. In order to solve the technical problems, the invention adopts the technical scheme that the MdCPP gene is applied to regulating and controlling the low-temperature stress of apples, the nucleotide sequence of a MdCPP gene coding region is shown as SEQ ID No. 1, and the amino acid sequence of a MdCPP gene coding region is shown as SEQ ID No. 2; by overexpressing MdCPP gene in apple callus, for reducing low temperature stress tolerance of the apple callus; or/and knocking down MdCPP genes in apple callus for enhancing low temperature stress tolerance of the apple callus. Preferably, when the MdCPP gene in apple callus is overexpressed, the fresh weight of apple callus is lower than that of wild-type apple callus under low temperature stress. Preferably, when the MdCPP gene in apple callus is knocked down, the fresh weight of apple callus is higher than that of wild apple callus under low temperature stress. Preferably, the temperature of the low temperature stress is 4 ℃. Compared with the prior art, the invention has the following advantages: The invention provides and verifies MdCPP genes for the first time that the low temperature stress resistance of apples can be regulated and controlled, and finds that the over-expressed MdCPP3 transgenic apple callus has poorer low temperature stress resistance, and the knockdown MdCPP transgenic apple callus has stronger low temperature stress resistance, and the genes not only provide a new theoretical view for analyzing the molecular mechanism of the cold resistance of apples, but also provide key functional gene targets and molecular design basis for realizing accurate and efficient breeding of the cold resistance of apples. The invention is described in further detail below with reference to the drawings and examples. Drawings FIG. 1 is a graph showing the relative expression levels of MdCPP genes in different tissues of apple in example 1 according to the present invention. FIG. 2 is a map of the running gel bands of MdCPP gene clones in example 1 of the present invention. FIG. 3 is a map of the identification of apple callus over-expressed with MdCPP gene in example 1 of the present invention. FIG. 4 is a diagram showing identification of apple callus with the MdCPP gene knocked down by gene editing in example 1 of the present invention. FIG. 5 is a low temperature resistant phenotype of MdCPP transgenic calli of example 1 of the present invention. FIG. 6 is a fresh weight map of MdCPP transgenic calli under low temperature stress at 25℃and 4℃in example 1 according to the present invention. Detailed Description Example 1 This example is the role of MdCPP gene in regulating apple low temperature stress. The polysaccharide polyphenol plant total RNA extraction reagent of this example was purchased from Tiangen Biotechnology (Beijing) Co., ltd., reverse transcription kit was purchased from Beijing full gold Biotechnology Co., ltd., product number AE311-02, DNA product purification kit was purchased from Jiangsu kang as century Biotechnology Co., ltd., product number CW2301M, pGM-T product cloning kit vector was purchased from Tiangen Biotechnology (Beijing) Co., pro