CN-121975853-A - Application of Zmpr gene in regulation and control of corn chloroplast development, photosynthesis, leaf morphology and yield

Abstract

The invention provides application of Zmpr gene in regulation and control of corn chloroplast development, photosynthesis, leaf morphology and yield, wherein the amino acid sequence of protein encoded by Zmpr gene is shown as SEQ ID NO. 2. The invention obtains two different mutation sites of the gene by screening corn mutants, which are respectively positioned in a promoter region and a 5' non-coding region, and discloses that the gene codes corn AAa-aPase enzyme for the first time, regulates and controls the development of chloroplasts, photosynthesis efficiency and double biological functions in leaf morphogenesis, and has wide application prospect in agricultural production.

Inventors

• DU DENGXIANG
• LI MENGTING
• LIN YING
• CHEN ZHIHUI

Assignees

• 武汉轻工大学

Dates

Publication Date

20260505

Application Date

20260320

Claims (5)

1. The application of Zmpr gene in regulating corn chloroplast development, photosynthesis, leaf morphology and yield is characterized in that the amino acid sequence of the Zmpr gene coded protein is shown as SEQ ID NO. 2.
2. 2. The use according to claim 1, wherein the cDNA sequence of Zmpr gene is shown in SEQ ID No. 1.
3. 3. A biological material comprising the Zmpr gene of any one of claims 1 or 2, said biological material comprising any one of an expression cassette, an expression vector, a transgenic cell line, or a recombinant bacterium.
4. 4. A maize mutant plant, characterized in that the Zmpr gene of the mutant plant has a mutation site selected from any one of (a) and (b), (A) A promoter region located at 126917944-12691745bp of chromosome 7 of maize, resulting in transposon insertion mutation; (b) The 5' non-coding region located at 126929499-126929500bp of chromosome 7 in maize, resulted in transposon insertion mutation.
5. 5. Use of Zmpr gene according to any one of claims 1 or 2 or the biological material according to claim 3 or the maize mutant plant according to claim 4 for maize improved breeding, seed production.

Description

Application of Zmpr gene in regulation and control of corn chloroplast development, photosynthesis, leaf morphology and yield Technical Field The invention relates to the technical field of molecular biology, in particular to application of Zmpr genes in regulation and control of corn chloroplast development, photosynthesis, leaf morphology and yield. Background AAA + atpases (atpases associated with various cellular activities) are a family of enzymes that are evolutionarily highly conserved, and are characterized in the core by the inclusion of a conserved ATP binding domain (AAA domain) consisting of 200-250 amino acids. The domain comprises a characteristic nucleotide binding site (Walker a motif) and a hydrolysis site (Walker B motif), wherein the conserved lysine residues in the Walker a motif are directly involved in the binding of ATP phosphate groups, mutations of which usually result in the loss of nucleotide binding capacity and complete inactivation of enzymatic activity, whereas the Walker B motif coordinates the hydrolysis reaction of ATP via acidic amino acid residues. Aaa+ proteins generally function biologically in the form of hexamers whose central cavities are formed by the arrangement of specific residues of each subunit, a critical region for substrate binding and processing. The family of proteins mediate a wide range of substrate conformational remodelling by transmitting mechanochemical energy to the substrate proteins through ATP binding and hydrolysis-driven conformational changes. According to the N-terminal linker (N-linker) sequence characteristics, the AAA+ family can be further divided into two subfamilies, carrying glycine-glycine sequences or hydrophobic residue-glycine sequences, respectively, and this structural difference is closely related to its functional diversity. In plant cells, aaa+atpase is deeply involved in the homeostatic maintenance of vital activities through both protein degradation and activity regulation. In terms of protein degradation, aaa+atpase is a core component of the ubiquitin/26S proteasome pathway, catalyzing the unfolding and transport of ubiquitinated substrates in an ATP-dependent manner, ensuring efficient clearance of damaged or misfolded proteins. The mechanism not only regulates basic physiological processes such as cell cycle, aging response and the like, but also plays a key role in the environment stress coping of plants. In terms of activity modulation, aaa+atpase directly regulates the catalytic efficiency of key metabolic enzymes through conformational remodeling, of which ribulose-1, 5-bisphosphate carboxylase/oxygenase activating enzyme (Rubisco activating enzyme, rca) is typically represented. Rca is a aaa+ family member, and the P-ring structure of its Walker a motif is a functional core that activates Rubisco activity. Studies show that Rca specifically removes the tight binding of a phosphate sugar inhibitor (such as 2-carboxyarabinitol-1-phosphate and ribulose-1, 5-biphosphoric acid) and an active site of Rubisco through ATP-driven conformational changes, and restores the carbamylation state and the catalytic function of the Rubisco, thereby maintaining the carbon fixation efficiency of the Calvin cycle. Although the mechanism of activation of Rubisco by Rca has been analyzed in greater detail in plants of arabidopsis, rice, etc., the molecular characteristics, genetic sequence diversity and tissue-specific expression regulation mechanisms of endogenous Rca and other aaa+atpase members have remained lacking systematic studies. The prior report shows that the activity of corn Rubisco is also regulated and controlled by the ATP dependency of the Rca, and environmental factors such as high temperature, high CO 2 concentration and the like obviously change the conformation and activation efficiency of the Rca by influencing the ATP/ADP ratio, thereby restricting the photosynthetic productivity. However, the complete set of genes encoding AAA+ATPase family in corn genome has not been completely identified, the division mechanism of different family members in the specific physiological processes of cell division, vascular development, grain filling and the like is not clear, and the study of separating cloning and functional verification of ATPase genes related to specific cell activities is still blank. This current situation limits the rational design of maize molecular breeding strategies based on modulation of atpase activity. Corn (Zea mays l.) is an important crop, has a high added value, and is widely cultivated worldwide. Since the advent of Mendelian's law of genetics, maize has been the classical mode crop of plant genetics research. The photosynthesis rate needs to be optimized, and functional genes for controlling important characters of corn are continuously and deeply researched, so that a foundation is laid for breeding new varieties of corn. Therefore, AAA+ATPase coding genes related to various cell activities are s