CN-121975854-A - Application and method of phyllostachys pubescens gene PheGBP in regulating and controlling root system configuration and promoting overground parts

Abstract

The invention discloses a phyllostachys pubescens plant regulation gene PheGBP, and application and a method thereof in regulating plant root system configuration and promoting plant overground part growth, wherein the gene is a DNA sequence in SEQ ID No. 1. The invention discovers that the gene is related to root system configuration and overground part growth for the first time, and can improve the expression of auxin synthetic genes, regulate and control the root system configuration of transgenic plants by over-expressing the gene in rice, thereby promoting the overground part growth of plants. The invention can be applied to crop genetic breeding, in particular to breeding for changing the root system configuration of plants and improving the growth of overground parts of plants.

Inventors

• WU MIN
• JIANG WENTAO
• SHAN XIN
• LIU YIDONG
• SI QIN

Assignees

• 安徽农业大学

Dates

Publication Date

20260505

Application Date

20260324

Claims (6)

1. 1. By over-expressing the phyllostachys pubescens gene PheGBP in wild rice, the root system configuration of the transgenic rice is changed and the application of the transgenic rice in promoting the growth of overground parts of the transgenic plant is promoted.
2. 2. The use according to claim 1, wherein the DNA sequence of the phyllostachys pubescens gene PheGBP is shown in SEQ ID No. 1.
3. 3. The use according to claim 1, wherein the root system is configured as main root, crown root and lateral root, and the overground part grows as plant height, lignin content and cellulose content in the stalks.
4. 4. A method for regulating and controlling root system configuration and promoting overground part growth is characterized in that the phyllostachys pubescens gene PheGBP is overexpressed, so that the root system configuration of transgenic plants is regulated and the overground part growth is promoted.
5. 5. The method of claim 4, wherein a plant over-expression vector is constructed, and the plant over-expression vector is a pCAMBIA1301-PheGBP plant expression vector with a RCc3 root-specific promoter, a phyllostachys pubescens gene PheGBP and a terminator in sequence in a region of a multiple cloning site.
6. 6. The method of claim 4, wherein the DNA sequence of the phyllostachys pubescens gene PheGBP is shown in SEQ ID No. 1.

Description

Application and method of phyllostachys pubescens gene PheGBP in regulating and controlling root system configuration and promoting overground parts Technical Field The invention relates to the field of plant molecular biology, in particular to a phyllostachys pubescens gene PheGBP7, and application and a method thereof in regulating and controlling plant root system configuration and promoting plant overground part growth. Background Bamboo is one of important forest resources, has the characteristics of wide distribution, fast growth, multiple purposes, high ecological and economic values and the like, and is known as green gold mine. Stem jointing is a remarkable biological property of bamboo, and the high growth of the overground part of the bamboo is indistinguishable from the developed underground tissue density. The underground tissue of bamboo mainly comprises bamboo roots and bamboo whips, the bamboo roots are main nutrition organs forming the underground system and are mainly responsible for absorbing water and inorganic salt in soil for the growth and development of overground parts of bamboo, so that the growth and development degree of root systems can directly influence the high growth of overground parts of bamboo forests. With the deep research of growth and development traits of bamboos, few genes regulating root system development in bamboos are identified. For example, GATA transcription factor PeGATA in Phyllostachys pubescens significantly suppresses the root length and plant height of transgenic Arabidopsis plants by promoting gibberellic acid. The 14-3-3b protein in the moso bamboo is over-expressed, compared with a control plant, the transgenic arabidopsis shows longer root system tissue, so that the diameter of stems of the transgenic arabidopsis becomes thicker, the bolting time is early, and the Pe14-3-3b protein promotes the growth of the plant by regulating and controlling the root system development. In addition, cai (2022) et al uses yeast double hybrid and double fluorescent molecule complementation experiments to indicate that PheGRF e can interact with PheIAA30, and in situ hybridization experiment results show that both play a role in root tip meristem and vascular bundle cells of stems. Phyllostachys Pubescens RNA m 6 A methyltransferases PheMTA and PheMTA2 regulate plant root development and salt stress resistance. The results show that the developed underground root system tissue not only can promote the growth of overground parts, but also can improve the tolerance of plants to (drought and high salt) environments. However, research on root development is currently relatively weak compared to other growth traits in bamboo. GBP (GAGA BINDING PROTEIN) proteins and BPC (BASIC PENTACYSTEINE) and BBR (BARLEY B RECOMBINANT) are homologous proteins. GBP transcription factors play an important role in regulating plant growth and development and the like. For example, in Arabidopsis, BPCs down-regulate SHOOTMERISTEMLESS (STM) and BREVIPEDICELLUS/KNAT1 (BP) gene expression, resulting in flower organ malformation. Meanwhile, the BPC family genes in arabidopsis have been reported to influence the growth and development of plant root systems. Compared to wild-type plants grown under the same conditions, BPC1-1, BPC2, BPC4, BPC6 mutant plants produced fewer lateral roots, BPC1-1, BPC2 and BPC4, BPC6 double mutant plants also had fewer lateral roots, but the phenotype was weaker than the BPC1-1, BPC2, BPC4, BPC6 mutants, which findings indicate that both four class I and II BPCs contribute to the formation of lateral roots. In rice, GAGA-binding transcription factors OsGBP and OsGBP3 regulate grain size and plant growth, and overexpression OsGBP1 results in dwarfing plants. In apples, suppression of MdYUC a and MdYUC b genes in transgenic apple plants overexpressing MdBPC2 resulted in reduced auxin accumulation, suppressed plant growth and altered tree structure. The research results show that the GBP transcription factor plays an important role in regulating and controlling the development of flower organs, root system configuration, plant height and the like. Disclosure of Invention The invention aims to provide a moso bamboo gene PheGBP and application and a method thereof in regulating and controlling plant root system configuration and promoting plant overground part growth. The invention protects application of changing the root system configuration of transgenic rice and promoting the growth of overground parts of transgenic plants by over-expressing phyllostachys pubescens gene PheGBP in wild rice, wherein the nucleotide sequence of phyllostachys pubescens gene PheGBP is shown as SEQ ID No.1, the root system configuration is main root, crown root and lateral root, and the growth of overground parts is plant height, lignin content and cellulose content in stems. The invention also protects a method for regulating and controlling the root system configuration and promoting the