CN-121975865-A - Use of GhANS related biologicals for improving plant salt tolerance

Abstract

The invention relates to the field of plant genetic engineering and biotechnology, in particular to application of GhANS related biological products in improving plant salt tolerance. The invention amplifies the full length of GhANS nucleotide sequence and constructs the full length on super1300-GFP carrier, and the carrier is expressed in tobacco transiently to determine that GhANS is protein positioned in cytoplasm and nucleus. After GhANS is silenced in cotton using VIGS techniques, cotton seedlings become more salt sensitive and have reduced antioxidant capacity. In addition, the transgenic technology is used for constructing the arabidopsis with over-expression GhANS, and compared with a control, the transgenic arabidopsis strain has stronger salt resistance and oxidation resistance.

Inventors

• LI FANGJUN
• WANG YUXIAN
• ZHOU LIN
• DU MINGWEI
• TIAN XIAOLI
• LI ZHAOHU

Assignees

• 中国农业大学

Dates

Publication Date

20260505

Application Date

20260317

Claims (8)

1. Use of a ghans-related biologic for improving salt tolerance in a plant, wherein the base sequence of GhANS is shown in SEQ ID No. 1.
2. 2. The use according to claim 1, wherein the biologic is a GhANS recombinant overexpression vector or recombinant engineering bacterium.
3. 3. The use according to claim 2, wherein said recombinant overexpression vector is obtained by inserting said GhANS into a pMDC vector.
4. 4. The use according to claim 2, wherein the recombinant agrobacterium is obtained after introduction of the recombinant overexpression vector into agrobacterium.
5. 5. The use according to claim 4, wherein the agrobacterium is GV3101.
6. 6. The use according to claim 2, wherein the recombinant over-expression vector is used to increase the expression level of GhANS to increase the salt tolerance of the plant.
7. 7. The use according to claim 6, wherein the method for improving salt tolerance of plants is as follows: Extracting RNA of cotton and reversely transcribing the RNA into cDNA; using cDNA as a template, cloning by using primers shown in SEQ ID NO.13 and SEQ ID NO.14 to obtain GhANS; GhANS is connected into a vector to obtain a recombinant over-expression vector; Transforming the recombinant over-expression vector into agrobacterium to obtain recombinant agrobacterium; And (3) infecting the plants by utilizing recombinant agrobacterium to obtain over-expression plants, namely improving the salt tolerance of the plants.
8. 8. The use according to claim 7, wherein the plant is arabidopsis thaliana or cotton.

Description

Use of GhANS related biologicals for improving plant salt tolerance Technical Field The invention relates to the field of plant genetic engineering and biotechnology, in particular to application of GhANS related biological products in improving plant salt tolerance. Background Salt stress is one of the major abiotic stresses affecting global agricultural production. The high salt environment can lead to increased osmotic pressure, enhanced ion toxicity and unbalanced nutrition of the soil, seriously inhibit the growth of crops, reduce the yield and quality, and destroy the soil structure. The salinization area in the cultivated land in China is large, and the salt damage becomes a key obstacle for restricting the sustainable development of agriculture. Cotton is an important commercial crop with relatively limited salt tolerance, and salt stress severely affects cotton planting area and yield stability. Therefore, the salt tolerance of plants, especially cotton, is improved, and has important significance for utilizing saline-alkali soil resources and guaranteeing agricultural output. At present, the approaches for improving the salt tolerance of plants mainly comprise traditional breeding, agricultural cultivation measures and modern biotechnology means. The traditional breeding method has long period, limited efficiency and limited germplasm resources, and agricultural improvement measures such as soil improvement, water management and the like have certain effects, but have high cost and are difficult to popularize and apply in a large area. Along with the rapid development of molecular biology and genetic engineering technology, the development of salt tolerance related genes of plants, and the cultivation of new salt tolerance varieties by genetic engineering means have become an important strategy for current stress-tolerant breeding. In recent years, studies have shown that multiple gene families play a key role in plants responding to salt stress, such as regulating ion balance, synthesizing osmoregulation substances, activating antioxidant systems, etc. However, the excavation and functional verification of key salt-tolerant genes in cotton are still relatively insufficient, and the progress of cotton salt-tolerant molecular breeding is restricted. Therefore, a new strategy for improving the salt tolerance of the cotton through the salt tolerance related genes of the cotton is needed to be provided, and the novel strategy has important theoretical value and application prospect for cultivating salt tolerance cotton germplasm and expanding saline-alkali land application. Disclosure of Invention In order to solve the problems, the invention provides an application of GhANS related biological products in improving salt tolerance of plants. The invention is realized by the following technical scheme: Use of GhANS related biological products for improving plant salt tolerance, wherein the base sequence of GhANS is shown as SEQ ID NO. 1. Preferably, the biological product is GhANS recombinant over-expression vector or recombinant engineering bacteria. Preferably, the recombinant overexpression vector is obtained by inserting the GhANS into a pMDC vector. Preferably, the recombinant agrobacterium is obtained after introduction of the recombinant overexpression vector into agrobacterium. Preferably, the agrobacterium is GV3101. Preferably, the recombinant over-expression vector is used for increasing the expression level of GhANS so as to improve the salt tolerance of the plant. Preferably, the method for improving salt tolerance of plants is as follows: RNA from cotton was extracted and reverse transcribed into cDNA. GhANS was obtained by cloning with the primers shown in SEQ ID No.13 and SEQ ID No.14 using cDNA as a template. GhANS was ligated into the vector to obtain a recombinant overexpression vector. And (3) transforming the recombinant over-expression vector into agrobacterium to obtain recombinant agrobacterium. And (3) infecting the plants by utilizing recombinant agrobacterium to obtain over-expression plants, namely improving the salt tolerance of the plants. Preferably, the plant is arabidopsis thaliana or cotton. Compared with the prior art, the invention has the following beneficial effects: The invention provides an application of GhANS related biological products in improving salt tolerance of plants, wherein the base sequence of GhANS is shown as SEQ ID NO. 1. The invention clones GhANS gene in cotton, and uses VIGS technology to silence the gene in cotton, which confirms the positive regulation effect of GhANS on salt-tolerant passage of cotton. Further, the vector of the over-expression GhANS is transformed into the Arabidopsis through an agrobacterium-mediated transformation method, and the result shows that the salt tolerance and the oxidation resistance of the over-expression Arabidopsis are improved. GhANS can be used as candidate gene for cotton stress-resistant molecular breeding