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CN-121975887-A - Preparation method and device of dispersed yeast glucan

CN121975887ACN 121975887 ACN121975887 ACN 121975887ACN-121975887-A

Abstract

The invention belongs to the technical field of functional polysaccharide preparation, and discloses a preparation method and a device of dispersed yeast glucan, the pretreatment modified non-water-soluble glucan is used as a raw material, and the product is prepared through the steps of sample configuration, enzymolysis, enzyme deactivation and post-treatment. The enzymolysis stage adopts a transmission assembly to drive stirring and a gas mixing assembly to be filled with inert gas to form micro-bubble cooperative treatment, the enzyme deactivation stage adopts a gradient heating mode and combines stirring and inert gas purging to realize efficient enzyme deactivation, the post-treatment adopts a closed-loop fractionation-reflux-predrying cooperative process, and the material treatment is completed by using devices such as a flow guide assembly, a cyclone separator and the like. The invention improves the dispersion uniformity of the glucan by the cooperation of multi-step processes and functional integration of the device while protecting the natural structural integrity of the glucan, is suitable for industrial production, and can be applied to the fields of foods, medicines and cosmetics.

Inventors

  • ZHANG YAN
  • PENG NING
  • CHEN ZHIXIAN
  • WANG TAO
  • LI YAJUN

Assignees

  • 安琪纽特股份有限公司
  • 安琪酵母股份有限公司

Dates

Publication Date
20260505
Application Date
20260209

Claims (10)

  1. 1. The preparation method of the dispersed yeast glucan is characterized by comprising the following steps of, (S01) sample preparation, namely adding pretreated modified water-insoluble glucan and water into a reaction kettle assembly, mixing, adding an acid-base regulator to adjust the pH value, and heating and stirring through a solution heating assembly to form a uniform pasty material; (S02) enzymolysis, namely after the temperature and the pH value of the materials obtained in the step (S01) are stable, adding an enzyme preparation into the reaction kettle assembly, driving and stirring through the transmission assembly, and simultaneously introducing inert gas into the reaction kettle assembly through the gas mixing assembly to form micro-bubble synergistic enzymolysis treatment; (S03) judging the enzymolysis end point, detecting the viscosity of materials in the reaction kettle component in the step (S02) at intervals, and entering the next step when the viscosity reaches the highest point and has a descending trend; (S04) enzyme deactivation, namely carrying out gradient heating on materials in the reaction kettle assembly in the step (S02) through a solution heating assembly, simultaneously maintaining stirring through a transmission assembly, continuously introducing inert gas by matching with a gas mixing assembly, and carrying out sectional heat preservation to realize enzyme deactivation; And (S05) post-treatment, namely conveying the materials subjected to enzyme deactivation in the step (S04) to a cyclone separator for primary gas-solid separation through a flow guide assembly, collecting solid materials, refluxing the solid materials subjected to primary separation to a reaction kettle assembly through the flow guide assembly, introducing preheated gas provided by a gas mixing assembly and an air heating assembly into the reaction kettle assembly, maintaining low-speed stirring of a transmission assembly for pre-drying treatment, repeating the separation-reflux-pre-drying operation for 1-3 times, conveying the materials to the cyclone separator for secondary separation through the flow guide assembly, and drying to obtain the dispersed yeast glucan powder.
  2. 2. The method for producing a dispersible yeast glucan according to claim 1, wherein the step (S01) of pretreating the modified water-insoluble glucan comprises the steps of, (A01) Placing non-water-soluble glucan into low-temperature plasma treatment equipment, introducing inert gas, and adjusting treatment power and treatment time to obtain plasma pretreatment glucan; (A02) Adding calcium salt water solution into the plasma pretreatment glucan in the step (A01), regulating the temperature of the system, and soaking at constant temperature to obtain a mineralization pretreatment system; (A03) And (3) carrying out solid-liquid separation on the mineralized pretreatment system in the step (A02), collecting a solid product, washing 3-5 times with purified water, then placing the solid product in vacuum drying equipment, drying the solid product under the conditions of a certain temperature and a vacuum degree, and collecting the solid product to obtain the pretreated modified non-water-soluble glucan.
  3. 3. The preparation method of the dispersed yeast glucan according to claim 2 is characterized in that the calcium salt in the step (A02) is one of calcium chloride, calcium lactate or calcium gluconate, the concentration of a calcium salt aqueous solution is 0.05 mol/L-0.2 mol/L, the mass-volume ratio of the plasma pretreatment glucan in the step (A02) to the calcium salt aqueous solution is 1g:10 mL-1 g:20mL, the temperature in the step (A03) is 45-60 ℃, the vacuum degree is 0.06-0.09 MPa, and the drying time is 2-4 h.
  4. 4. The preparation method of the dispersed yeast glucan, which is disclosed in claim 1, is characterized in that the solid content of the material obtained by mixing the pretreated modified non-water-soluble glucan with water in the step (S01) is 5% -15%, the acid-base regulator in the step (S01) is one or more selected from sodium hydroxide, potassium hydroxide, acetic acid, lactic acid, hydrochloric acid and citric acid, the pH value of the enzyme preparation in the step (S01) is adjusted to 4.0-7.0, the enzyme preparation in the step (S02) is one or more selected from protease, amylolytic enzyme, pectolyase and cellulolytic enzyme, the protease in the step (S02) is one or more selected from papain, bromelain, trypsin and pepsin, the amylolytic enzyme in the step (S02) is one or more selected from alpha-amylase, beta-amylase and high-temperature amylase, the pectolyase is one or more selected from pectolyase, pectolyase and pectolyase in the step (S02) is one or more selected from cellulolytic enzyme, the enzyme and the enzyme in the step (S02) is one or more selected from cellulolytic enzyme and the enzyme.
  5. 5. The preparation method of the dispersed yeast glucan is characterized in that the addition amount of an enzyme preparation in the step (S02) is 0.5-2% of the solid content in the obtained material, the enzymolysis temperature in the step (S02) is 45-60 ℃, inert gas introduced into a gas mixing assembly in the step (S02) is argon or nitrogen, the flow is 0.1-0.5L/min, the pressure is 0.02-0.05 MPa, the stirring mode of a transmission assembly in the step (S02) is stepwise gradient alternating stirring, the stirring speed of the transmission assembly is 50-80 r/min for 1-2 h before enzymolysis, the stirring speed of the subsequent enzymolysis stage is 100-150 r/min, and the stirring direction is switched every 30 min.
  6. 6. The preparation method of the dispersed yeast glucan, which is disclosed in claim 1, is characterized in that the set time in the step (S03) is 0.5-1 h, the viscosity detection method in the step (S03) is that a material is sucked by a 5ml pipette, the time required for the material to fall by 4ml is measured to judge the change of viscosity, the temperature in the first stage of gradient heating in the step (S04) is 55-65 ℃ and the heat preservation time is 10-15 min, the temperature in the second stage of gradient heating in the step (S04) is 70-90 ℃ and the heat preservation time is 30-60 min, the flow rate of inert gas introduced into a gas mixing assembly in the step (S04) is 0.3-0.8L/min, and the stirring speed of a transmission assembly in the step (S04) is 60-90 r/min.
  7. 7. The preparation method of the dispersed yeast glucan, which is disclosed in claim 1, is characterized in that the preheating gas temperature in the step (S05) is 60-80 ℃, the stirring speed of a transmission assembly in the pre-drying treatment in the step (S05) is 30-50 r/min, the flow rate of the preheating gas introduced into a gas mixing assembly in the step (S05) is 0.6-1.2L/min, the volume ratio of purified water to materials and purified water in the step (S05) is 1:1-3, the final drying mode in the step (S05) is spray drying or freeze drying, and the drying temperature of the materials after secondary separation in the step (S05) is 80-120 ℃.
  8. 8. The preparation device of the dispersed yeast glucan comprises a reaction kettle assembly (100) and is characterized in that a gas mixing assembly (200) is arranged at the bottom of the reaction kettle assembly (100), a flow guiding assembly (300) is arranged at the bottom of the gas mixing assembly (200), one end of the flow guiding assembly (300) is connected to the top of the reaction kettle assembly (100) through a pipeline, a cyclone separator (400) is connected to the other end of the flow guiding assembly (300) through a pipeline, the bottom end of the gas mixing assembly (200) is communicated with the reaction kettle assembly (100) and the cyclone separator (400) through the flow guiding assembly (300), two groups of solution heating assemblies (500) are attached to the surface of the reaction kettle assembly (100), two groups of air heating assemblies (600) are respectively communicated to the right sides of the two groups of solution heating assemblies (500), a transmission assembly (700) is movably connected between the air heating assemblies (600) and the flow guiding assembly (300), the reaction kettle assembly (100) comprises a reaction kettle body (101), an air outlet (106) is formed in the top of the reaction kettle body, an air outlet (106) is formed in the reaction kettle body, and an activated carbon (107) is arranged in the reaction kettle body, the gas mixing assembly (200) comprises a mixing cylinder (201) arranged right below a reaction kettle body (101), a gas guide cover (202) is fixedly penetrated at the center of the top of the mixing cylinder (201), a centrifugal atomizer (203) is fixedly penetrated at the center of the gas guide cover (202), a gas pipe (204) is fixedly arranged on the side face of the gas guide cover (202), and the bottom end of the gas guide cover (202) is sleeved outside the bottom output end of the centrifugal atomizer (203).
  9. 9. The preparation facilities of dispersion type yeast glucan of claim 8, wherein solution heating element (500) is including two sets of semicircle stock solution shell (501) that distribute around, two sets of semicircle stock solution shell (501) are inside to be filled with silicon oil, semicircle stock solution shell (501) surface mounting has heater (502), heater (502) are used for heating silicon oil, semicircle stock solution shell (501) are used for laminating at reation kettle body (101) surface and heat reation kettle body (101), semicircle stock solution shell (501) top fixed mounting has outlet pipe (503), outlet pipe (503) bottom fixed mounting has inlet pipe (504), semicircle stock solution shell (501) both sides all fixed mounting have connecting plate (505), and two sets of guide bars (506) of homonymy run through sliding connection on connecting plate (505), all fixed mounting anchor plate (507) between the two sets of guide bars (506) tip of homonymy, and rotate between two sets of anchor plates (507) of homonymy and heat reation kettle body (101), threaded rod (508) the screw thread hole (508) are seted up in opposite directions on threaded rod (505), threaded connection is threaded connection in the opposite directions on threaded rod (505).
  10. 10. The device for preparing the dispersed yeast glucan according to claim 9, wherein the air heating assembly (600) comprises two groups of semicircular liquid storage shells II (605) which are attached to the front side and the rear side of the air pipe (204), communication pipes (606) are fixedly arranged at two ends of the semicircular liquid storage shells II (605), the semicircular liquid storage shells II (605) are respectively connected with the oil outlet pipe (503) and the oil inlet pipe (504) through the two communication pipes (606), and silicone oil in the semicircular liquid storage shells I (501) flows into the semicircular liquid storage shells II (605) through the oil outlet pipe (503) and flows back into the semicircular liquid storage shells I (501) through the oil inlet pipe (504); The semicircular liquid storage shell comprises a semicircular liquid storage shell (605), a semicircular liquid storage shell (607) and a sliding rod (608) are fixedly arranged at the other end of the semicircular liquid storage shell (607), the sliding rod (608) is parallel to the top of the semicircular liquid storage shell (605), a push plate (609) is connected to the guide groove (607) in a sliding mode, the sliding rod (608) penetrates through the push plate (609), a first spring (610) is fixedly arranged between the push plate (609) and the end portion of the guide groove (607), the first spring (610) is used for driving the push plate (609) to be close to the semicircular liquid storage shell (605), and the two groups of push plates (609) are fixedly arranged on connecting plates (505) on the right sides of the semicircular liquid storage shells (501) respectively.

Description

Preparation method and device of dispersed yeast glucan Technical Field The invention belongs to the technical field of functional polysaccharide preparation, and particularly relates to a preparation method and device of dispersed yeast glucan. Background Yeast beta-glucan is core functional polysaccharide of yeast cell walls, has various biological activities such as immunoregulation, antioxidation and the like, and has wide application prospects in the fields of foods, medicines, cosmetics and the like. However, the natural compact triple helix structure and the larger molecular weight thereof lead to poor water solubility and dispersibility, which makes it difficult to fully exert biological activity, therefore, the dispersibility is improved through a mild and efficient preparation and modification technology, and the method becomes a key research direction in the field. The preparation of existing yeast glucans generally extends around pretreatment, extraction, modification and post-treatment of the raw materials. The method mainly comprises the steps of pretreatment of raw materials, namely crushing yeast cell walls by adopting modes of autolysis, ball milling, ultrasonic or high-pressure homogenization and the like, wherein the modification means comprise physical modification, chemical modification and biological modification, wherein the physical modification usually damages a molecular structure through mechanical actions of high-pressure microjet, ultrasonic and the like, the chemical modification introduces hydrophilic groups through carboxymethylation, sulfation and the like, the biological modification mainly adopts an enzymolysis technology, and part of the process adopts various methods for cooperative treatment, such as enzymolysis and high-pressure microjet combination, ultrasonic assisted enzymolysis and the like. The post-treatment usually involves steps such as separation, washing and drying to obtain the target product. The prior art can improve the dispersibility of glucan to a certain extent, but still has the defects. The reaction conditions of partial chemical modification methods are relatively harsh, or exogenous impurities can be introduced, natural bioactive structures of glucan are easily damaged due to overlarge shearing force in the physical modification process, and even the synergistic modification process has room for optimization in terms of product dispersion uniformity, structural integrity and industrial production efficiency, so that development of a preparation technology which is milder, efficient and suitable for industrialization is needed to improve the product quality and application potential of the dispersed yeast glucan. In addition, in the preparation process of the yeast glucan, a reaction kettle is needed, acid liquor is needed to be added into the reaction kettle to adjust the pH, protease, amylase, cellulase and the like are needed to be added for enzymolysis, but when different enzymes are added for enzymolysis, the required temperature is different, the sample is needed to be heated or cooled at different stages, and after the sample is heated, the temperature is cooled again, the cooling process needs longer time, so that the preparation efficiency of the yeast glucan is reduced, and the defects that the reaction kettle cannot be used for enzymolysis can be further improved. Disclosure of Invention In order to overcome the defects in the prior art, the preparation method and the device for the dispersed yeast glucan solve the problem that the uniformity of glucan dispersion, the structural integrity of natural biological activity and the suitability for industrial production are difficult to consider in the prior art. The first technical scheme of the invention is that the preparation method of the dispersed yeast glucan comprises the following steps, (S01) sample preparation, namely adding pretreated modified water-insoluble glucan and water into a reaction kettle assembly, mixing, adding an acid-base regulator to adjust the pH value, and heating and stirring through a solution heating assembly to form a uniform pasty material; (S02) enzymolysis, namely after the temperature and the pH value of the materials obtained in the step (S01) are stable, adding an enzyme preparation into the reaction kettle assembly, driving and stirring through the transmission assembly, and simultaneously introducing inert gas into the reaction kettle assembly through the gas mixing assembly to form micro-bubble synergistic enzymolysis treatment; (S03) judging the enzymolysis end point, detecting the viscosity of materials in the reaction kettle component in the step (S02) at intervals, and entering the next step when the viscosity reaches the highest point and has a descending trend; (S04) enzyme deactivation, namely carrying out gradient heating on materials in the reaction kettle assembly in the step (S02) through a solution heating assembly, simultaneously maintainin