CN-121975893-A - Chicken peptide protein reactant capable of preventing or relieving alcoholic liver injury and synthetic peptide and application thereof

Abstract

The invention discloses a chicken peptide protein reactant capable of preventing or relieving alcoholic liver injury, and synthetic peptide and application thereof. According to the invention, protease and tryptophan are added into the chicken enzymolysis product, and the protease is deactivated after the protease-like reaction, so that the prepared chicken peptide protein reactant has better ADH stabilizing activity and antioxidant activity, and compared with the ADH stabilizing activity and antioxidant activity of the chicken enzymolysis product CP, the chicken peptide protein reactant has remarkable improvement, and meanwhile, the digestion stability of chicken peptide is improved, and the chicken peptide protein reactant has a better effect of preventing and treating alcoholic liver injury. Further analyzing peptide fragments by mass spectrometry, and identifying 6 synthesized peptides from chicken peptide protein reactants has better ADH stabilizing activity and antioxidant activity. The invention provides more sources and choices for protein polypeptide substances with ADH activity and antioxidant activity, and the preparation method is simple, convenient and quick, has mild reaction conditions, and can be better used for preparing products for preventing and treating alcoholic liver injury.

Inventors

• Xiao Chuqiao
• Zeng Hujiaan
• XIAO JIE
• Lang Jizhou
• LI XIANGGUANG

Assignees

• 广东工业大学

Dates

Publication Date

20260505

Application Date

20251226

Claims (10)

1. 1. A chicken peptide protein reactant is characterized in that chicken is subjected to enzymolysis by using 0.5-2% of protease to obtain a chicken enzymolysis product, 0.01-10% of protease and 1-10% of tryptophan are added into 25-60% of chicken enzymolysis product, the protease is deactivated after the protease-like reaction, and the chicken is centrifuged, and supernatant fluid is obtained.
2. 2. The preparation method of the chicken peptide protein reactant is characterized by comprising the steps of crushing chicken, adding distilled water with the volume of 3-5 times, mixing, adjusting the pH value to 7.9-8.1, adding 0.5-2% protease for enzymolysis, inactivating enzyme, centrifuging, taking supernatant to obtain a chicken enzymolysis product, adding 0.01-10% protease and 1-10% tryptophan into the chicken enzymolysis product, adjusting the pH value to 6-11, vibrating and hydrolyzing for 0.5-72h at 15-70 ℃, inactivating enzyme after the protein-like reaction, centrifuging, and taking supernatant to obtain the chicken peptide protein reactant.
3. 3. The method of claim 2, wherein the protease is selected from alkaline protease, neutral protease, or papain.
4. 4. The method of claim 2, wherein the protease is present at a concentration of 0.1-5%.
5. 5. The method according to claim 2, wherein the tryptophan has a concentration of 1 to 6%.
6. 6. Use of a chicken peptide protein reactant as defined in claim 1 for the preparation of an ADH-activated product and/or an antioxidant product.
7. 7. Use of the chicken peptide protein reactant of claim 1 in the preparation of a product for preventing or alleviating alcoholic liver injury.
8. 8. A synthetic peptide characterized by containing a peptide fragment AAW, WAF, VVWF, YFF, TLW, WLL identified from the chicken peptide protein reactant of claim 1.
9. 9. Use of a synthetic peptide according to claim 8 for the preparation of an ADH-activated product and/or an antioxidant product.
10. 10. An ADH-activated product and/or an antioxidant product comprising the chicken peptide protein reactant of claim 1 or the synthetic peptide of claim 8.

Description

Chicken peptide protein reactant capable of preventing or relieving alcoholic liver injury and synthetic peptide and application thereof Technical Field The invention relates to the technical field of polypeptide preparation and biological medicine, in particular to a chicken peptide protein reactant capable of preventing or relieving alcoholic liver injury, and synthetic peptide and application thereof. Background ADH (alcohol dehydrogenase) is a key enzyme for alcohol metabolism, and its activity directly affects the rate of alcohol decomposition and the tolerance of the body to alcohol. The human with high ADH activity has fast alcohol metabolism, less accumulation of acetaldehyde and lower risk of damage to liver and nervous system. While long-term consumption of alcohol in large amounts inhibits ADH activity. At present, medical intervention such as the use of liver protection drugs (such as silybin and compound glycyrrhizin) can indirectly improve the enzyme activity, thereby improving the alcohol metabolism capability, reducing the physical burden and maintaining the normal liver function. Alcoholic liver injury is closely related to the metabolic capacity of ADH. The key causes of alcoholic liver injury are direct toxicity of acetaldehyde, oxidative stress and inflammation and metabolic disorders. As the activity of ADH is inhibited, acetaldehyde accumulation is caused, and at the same time, glycolipid metabolism is disturbed, liver fibrosis is accelerated. Thus, reducing the accumulation of acetaldehyde by activating ADH activity can prevent or alleviate alcoholic liver injury, particularly acute alcoholism. Meanwhile, oxidative stress can promote hepatic stellate cell activation and accelerate fibrosis progress by generating a large amount of free radicals and destroying cell structures to trigger inflammation and apoptosis, so that the liver is further damaged. The prior studies disclose protein polypeptides for ADH activation as Qiya seed meal protein hydrolysates, zein, seaweed (Cyrtymenia Sparsa) proteins, whole weever protein hydrolysates, and the like. However, most studies have focused on either ADH activating activity (e.g., anti-hangover and liver-protecting direction) or antioxidant activity (e.g., anti-aging and anti-inflammatory directions). Currently, there are few studies and reports on protein polypeptides that can be used for both ADH (alcohol dehydrogenase) activation and antioxidant. Therefore, in order to further enhance the stabilizing and antioxidant activities of ADH, there is a need to develop more and more efficient protein polypeptide products that can be better used for preventing or alleviating alcoholic liver injury, and thus the present application was filed. Disclosure of Invention The invention aims to solve the technical problems that the existing substances with ADH stabilizing activity and oxidation resistance effect are insufficient, and ADH stabilizing activity and oxidation resistance effect of chicken source polypeptide are improved. It is a first object of the present invention to provide a chicken peptide protein reactant. The second object of the invention is to provide a preparation method of chicken peptide protein reactant. A third object of the present invention is to provide the use of chicken peptide protein reactants. It is a fourth object of the present invention to provide a synthetic peptide. It is a fifth object of the present invention to provide the use of synthetic peptides. A sixth object of the invention is to provide a product. The above object of the present invention is achieved by the following technical scheme: The invention provides a chicken peptide protein reactant, which is prepared by adopting 0.5-2% of protease to carry out enzymolysis on chicken to obtain a chicken enzymolysis product, adding 0.01-10% of protease and 1-10% of tryptophan into 25-60% of chicken enzymolysis product, carrying out protease-like reaction, inactivating enzyme, centrifuging, and taking supernatant. The invention carries out a plastein reaction on chicken source polypeptide, and the protein-like reactant prepared by adding exogenous amino acid has good ADH stabilizing activity and antioxidant activity, and compared with the ADH stabilizing activity and antioxidant activity of chicken enzymolysis product CP, the protein-like reactant has remarkable improvement. The ADH stabilizing activity of the chicken peptide protein reactant provided by the invention is raised to 134.68 +/-0.64% of the CP, the ABTS free radical scavenging activity is raised to 241.10 +/-2.95% of the CP, and the DPPH free radical scavenging activity is raised to 209.64 +/-2.20% of the CP. Meanwhile, the digestion stability of chicken peptide is improved, and the chicken peptide has better effect of preventing and treating alcoholic liver injury and can be used for relieving inflammation caused by alcoholic liver injury. Analysis by chemical driving force shows that the hydrogen bond and