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CN-121975894-A - Weever collagen peptide and preparation method and application thereof

CN121975894ACN 121975894 ACN121975894 ACN 121975894ACN-121975894-A

Abstract

The invention relates to the field of bioengineering, in particular to a weever collagen peptide, a preparation method and application thereof. The invention provides a preparation method of weever collagen, which comprises the following steps of acid extraction of pretreated weever scales, first enzyme hydrolysis, filtration, salting out of filtrate, centrifugation, collection of sediment, re-dissolution, purification, second enzyme hydrolysis, and heating to obtain the weever collagen, wherein enzymes adopted by the first enzyme hydrolysis and the second enzyme hydrolysis can be the same or different, and the enzymes comprise one or more of alkaline protease, papain, subtilisin and pepsin. The collagen peptide extracted from the sea bass fish scales and processed into the collagen peptide has excellent anti-photoaging effect and strong replicability. The collagen peptide has low molecular weight, strong antioxidant capacity and wide application range, and can be used in the fields of cosmetics, oral collagen supplements and the like.

Inventors

  • XU BAOJUN
  • LIU DONGCHENG

Assignees

  • 北师香港浸会大学

Dates

Publication Date
20260505
Application Date
20260203

Claims (10)

  1. 1. The preparation method of the weever collagen is characterized by comprising the following steps of acid extraction of pretreated weever scales, first enzyme hydrolysis, filtration, salting out of filtrate, centrifugation, collection of sediment, re-dissolution, purification, second enzyme hydrolysis, and heating to obtain the weever collagen; The enzymes used for the first enzymatic hydrolysis and the second enzymatic hydrolysis may be the same or different; the enzyme comprises one or more of alkaline protease, papain, subtilisin and pepsin.
  2. 2. The method of claim 1, wherein the first enzymatic hydrolysis is pepsin and the second enzymatic hydrolysis is alkaline protease.
  3. 3. The preparation method according to claim 1 or 2, wherein the pretreatment comprises a first soaking and a second soaking, wherein the first soaking adopts 0.1-0.2 mol/L sodium hydroxide solution, and the second soaking adopts 0.5-0.6 mol/L EDTA-2Na.
  4. 4. The method according to any one of claims 1 to 3, wherein the acid extraction is performed using one or more of citric acid, glacial acetic acid and hydrochloric acid in an amount of 0.5 to 0.6 mol/L.
  5. 5. The method of any one of claims 1 to 4, wherein the weever collagen has a molecular weight of less than 3kDa.
  6. 6. The weever collagen obtained by the preparation method according to any one of claims 1 to 5.
  7. 7. The weever collagen of claim 6, comprising a peptide composition; the peptide fragment composition has: (1) The amino acid sequence shown as SEQ ID NO. 1 to SEQ ID NO. 18, or (2) An amino acid sequence obtained by substituting, deleting or adding one or more amino groups to the amino acid sequence shown in (1) and having the same or similar function as the amino acid sequence shown in (1), or (3) An amino acid sequence having at least 80% identity to the amino acid sequence of (1) or (2).
  8. 8. Use of the weever collagen according to claim 6 or 7 for the preparation of a product resistant to photo-aging.
  9. 9. Use of the weever collagen according to claim 6 or 7 for the preparation of an antioxidant product.
  10. 10. The product is characterized by comprising the weever collagen according to claim 6 or 7 and acceptable auxiliary agents and/or auxiliary materials.

Description

Weever collagen peptide and preparation method and application thereof Technical Field The invention relates to the field of bioengineering, in particular to a weever collagen peptide, a preparation method and application thereof. Background The annual output of weever in China is over 90 ten thousand tons, and the output in Guangdong area is the largest. Of these yields, only a small fraction of weever is delivered directly to the aquaculture market for sale as a fresh product after harvesting. Most weever is required to be processed by a factory in a three-step process, namely fish scales, fishbone and viscera removal, and transported to various places in the country in a vacuum freezing preservation mode. The processing and preserving modes not only can ensure the freshness of weever, but also can improve the convenience and efficiency of transportation. However, in this process, a large amount of fish by-products is not fully utilized. These byproducts include fish scales, fish bones, fish viscera, etc., which are often processed into low value products such as pet food additives and aquaculture feeds due to their high protein content. Although this treatment approach solves some of the by-product issues, it does not fully exploit its potential economic and nutritional value. In addition, due to the high cost of transportation and storage of the byproducts, some plants choose to directly bury the byproducts in situ. This approach not only wastes valuable resources, but also creates serious environmental problems. The fish byproducts can generate a large amount of organic wastes in the burying process, so that soil and water body are polluted, and the surrounding ecological environment and resident life are influenced. Therefore, how to effectively utilize the byproducts generated in the weever processing process, improve the added value of the weever, reduce the environmental pollution and become a urgent problem to be solved. Through technical innovation and industrial upgrading, more high-value utilization ways can be explored, so that recycling and sustainable development of resources are realized. Currently, the mainstream collagen products are usually extracted from pig skin and bovine Achilles tendon, but these sources are at risk of contamination by biological pathogens. The biosynthesis of collagen is not expected due to structural loss. Thus, collagen extracted from fish becomes particularly important. The current research has mainly focused on the extraction of collagen from fish skin, in particular tilapia. However, for weever sold in whole fish, removal of the skin can greatly reduce the price of the whole fish, which is not appreciated. Therefore, other processing byproducts need to be considered. The protein content of the weever thorns is low, and the weever thorns are not suitable for extracting collagen, and the weever viscera are easy to rancidity and deteriorate due to high fat content when the transportation and storage conditions are not up to standard. In contrast, the weever scales are rich in collagen, and under normal processing conditions, the protein is more stable than the fat, making it the best candidate for the utilization of weever byproducts. In addition, mature fish scale collecting equipment is already available in the existing processing process, and the weever scales can be obtained in a large quantity, so that the method has great research potential. Collagen products are used for treating skin aging problems, and are mainly inspired by the traditional concept of "instant food". It has long been believed that by taking foods containing specific ingredients, the nutrition required by the body can be directly supplemented, thereby improving the corresponding health problems. Collagen, an important structural protein, has naturally become a popular choice for improving skin health. With the intensive research, it has been gradually discovered that collagen peptides, i.e., products of hydrolyzed collagen, have superior properties to the original collagen. The collagen peptide not only maintains the basic functions of the collagen, but also has stronger effects on the aspects of antioxidation, anti-inflammation, anti-aging and the like. This makes it easier for the hydrolyzed collagen peptide to be absorbed and utilized by the intestinal tract due to its lower molecular weight, thereby exerting its biological activity more effectively. Based on these findings, collagen peptides are widely used in various oral skin care products such as soft sweets, oral liquids, and the like. The products help improve the elasticity and moisture of the skin and slow down the aging process of the skin by taking orally. In addition, collagen peptides are also added to skin care products such as facial masks and skin creams that are applied directly to the skin. These topical products provide topical nutritional support and repair by direct contact with the skin, further enhancing the healt