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CN-121975895-A - Jellyfish crude polypeptide and jellyfish polypeptide with tyrosinase inhibitory activity and preparation methods and application thereof

CN121975895ACN 121975895 ACN121975895 ACN 121975895ACN-121975895-A

Abstract

The invention discloses jellyfish crude polypeptide with tyrosinase inhibitory activity, jellyfish polypeptide and a preparation method and application thereof, and belongs to the technical field of biology. The jellyfish crude polypeptide is prepared and identified and optimized, and the jellyfish polypeptide GGDYLSIHMRR is verified by experiments that both the jellyfish crude polypeptide and the jellyfish polypeptide provided by the invention can inhibit tyrosinase activity in vitro, and can inhibit melanin secretion of B16-F10 mouse melanoma cells and inhibit intracellular tyrosinase activity. Therefore, the jellyfish crude polypeptide and the jellyfish polypeptide can be used as tyrosinase inhibitors and anti-melanin synthesis agents and applied to the fields of cosmetics, medical treatment and the like. The jellyfish crude polypeptide and jellyfish polypeptide provided by the invention are proved to have no cytotoxicity through experiments, are derived from the ocean, and have no land-based animal disease risk and religion barrier.

Inventors

  • YU HUAHUA
  • Sheng Juhua
  • WANG WEIFENG
  • LI PENGCHENG

Assignees

  • 水母娘娘海洋生物科技有限公司
  • 中国科学院海洋研究所

Dates

Publication Date
20260505
Application Date
20260204

Claims (9)

  1. 1. A method for preparing jellyfish crude polypeptide with tyrosinase inhibitory activity, which is characterized by comprising the following steps: The jellyfish is dehydrated and desalted to obtain a jellyfish dry product; Sequentially carrying out enzymolysis treatment on the jellyfish dried product by using composite protease, alkaline protease and flavourzyme, and collecting supernatant after enzymolysis; And (5) freeze-drying the supernatant to obtain the jellyfish crude polypeptide.
  2. 2. The method according to claim 1, wherein the enzyme addition amount of the compound protease is 4000U/g, the enzyme is hydrolyzed for 8 hours under the condition of 50℃, pH and 7.0, the enzyme addition amount of the alkaline protease is 2000U/g, the enzyme is hydrolyzed for 8 hours under the condition of 60℃, pH and 9.5, and the enzyme addition amount of the flavor protease is 3000U/g, and the enzyme is hydrolyzed for 6 hours under the condition of 40℃, pH and 7.0.
  3. 3. A crude jellyfish polypeptide produced by the production process according to any one of claims 1 to 2.
  4. 4. A jellyfish polypeptide with tyrosinase inhibitory activity is characterized in that the amino acid sequence of the jellyfish polypeptide is shown as SEQ ID NO. 5.
  5. 5. Use of a crude jellyfish polypeptide of claim 3 or a jellyfish polypeptide of claim 4 in any one of the following: (1) Preparing a tyrosinase inhibitor; (2) Preparing a whitening and skin-protecting cosmetic; (3) Preparing a health product for improving chloasma; (4) Preparing the medicine for treating skin pigmentation diseases.
  6. 6. A tyrosinase inhibitor comprising the jellyfish crude polypeptide of claim 3 or the jellyfish polypeptide of claim 4.
  7. 7. A cosmetic for whitening and caring skin, comprising the jellyfish crude polypeptide of claim 3 or the jellyfish polypeptide of claim 4.
  8. 8. A health product for improving chloasma, which is characterized by comprising the jellyfish crude polypeptide of claim 3 or the jellyfish polypeptide of claim 4.
  9. 9. A medicament for treating skin pigmentation disorders, comprising the jellyfish crude polypeptide of claim 3 or the jellyfish polypeptide of claim 4.

Description

Jellyfish crude polypeptide and jellyfish polypeptide with tyrosinase inhibitory activity and preparation methods and application thereof Technical Field The invention relates to the technical field of biology, in particular to jellyfish crude polypeptide with tyrosinase inhibitory activity, jellyfish polypeptide, and a preparation method and application thereof. Background Melanin is a substance produced by normal human cells to balance skin color and resist skin irritation and damage caused by ultraviolet rays, and is the first barrier for protecting human skin and weakening photoaging, and is mainly present in keratinocytes and melanocytes. Normal production of melanin helps to protect the skin from uv damage, but abnormal accumulation of melanin can lead to skin problems such as chloasma, freckles and post-inflammatory pigmentation, and even can induce melanoma. Tyrosinase is the rate-limiting enzyme in the process of melanin formation, and participates in two main processes in catalyzing melanin synthesis, first in catalyzing tyrosine formation of 3, 4-dihydroxyphenylalanine (dopa) in the human body. After the dopa is formed, tyrosinase further shows the activity of bisphenol enzyme, and the dopa is catalyzed to continuously synthesize dopa quinone, then substances such as 5, 6-dihydroxyindole, 5, 6-dihydroxyindole carboxylic acid and the like are synthesized, and finally brown pigment and melanin are formed through oxidative polymerization. Therefore, the purpose of inhibiting melanin synthesis can be achieved by inhibiting tyrosinase activity. Currently, whitening cosmetics and medicines often contain chemical whitening components, such as kojic acid, hydroquinone, nicotinamide and the like, which have certain irritation and cytotoxicity, and can weaken skin barrier, damage sensitive skin and even risk causing cancer. In recent years, the number of jellyfish is increased in an explosive manner under the influence of factors such as global warming, eutrophication and the like, and the main component of jellyfish organic matters is protein, so that the jellyfish is a high-quality raw material of active polypeptide. However, jellyfish polypeptides having a whitening effect have been rarely studied at present. Disclosure of Invention The invention aims to provide jellyfish crude polypeptide and jellyfish polypeptide with tyrosinase inhibitory activity, as well as preparation methods and application thereof, so as to solve the problems of the prior art, and the jellyfish crude polypeptide and jellyfish polypeptide provided by the invention can inhibit tyrosinase activity in vitro, inhibit melanin secretion of B16-F10 mouse melanoma cells and inhibit intracellular tyrosinase activity. In order to achieve the above object, the present invention provides the following solutions: the invention provides a preparation method of jellyfish crude polypeptide with tyrosinase inhibitory activity, which comprises the following steps: The jellyfish is dehydrated and desalted to obtain a jellyfish dry product; Sequentially carrying out enzymolysis treatment on the jellyfish dried product by using composite protease, alkaline protease and flavourzyme, and collecting supernatant after enzymolysis; And (5) freeze-drying the supernatant to obtain the jellyfish crude polypeptide. Optionally, the enzyme adding amount of the compound protease is 4000U/g, the enzymolysis is carried out for 8 hours under the condition of 50℃, pH and 7.0, the enzyme adding amount of the alkaline protease is 2000U/g, the enzymolysis is carried out for 8 hours under the condition of 60℃, pH and 9.5, the enzyme adding amount of the flavor protease is 3000U/g, and the enzymolysis is carried out for 6 hours under the condition of 40℃, pH and 7.0. The invention also provides jellyfish crude polypeptide prepared by the preparation method. The invention also provides jellyfish polypeptide with tyrosinase inhibitory activity, and the amino acid sequence of the jellyfish polypeptide is shown as SEQ ID NO. 5. The invention also provides the application of the jellyfish crude polypeptide or the jellyfish polypeptide in any one of the following: (1) Preparing a tyrosinase inhibitor; (2) Preparing a whitening and skin-protecting cosmetic; (3) Preparing a health product for improving chloasma; (4) Preparing the medicine for treating skin pigmentation diseases. The invention also provides a tyrosinase inhibitor, which comprises the jellyfish crude polypeptide or the jellyfish polypeptide. The invention also provides a cosmetic for whitening and protecting skin, which comprises the jellyfish crude polypeptide or the jellyfish polypeptide. The invention also provides a health product for improving chloasma, which comprises the jellyfish crude polypeptide or the jellyfish polypeptide. The invention also provides a medicine for treating skin pigmentation diseases, which comprises the jellyfish crude polypeptide or the jellyfish polypeptide. The invention discloses