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CN-121975898-A - Deer myocardial peptide and preparation method and application thereof

CN121975898ACN 121975898 ACN121975898 ACN 121975898ACN-121975898-A

Abstract

The invention relates to the field of small molecular peptides and discloses deer myocardial peptide, a preparation method and application thereof, wherein the preparation method comprises the following steps of thawing frozen deer heart, removing fascia, a tube and fat, cutting into blocks, cleaning and mincing into minced meat; performing enzymolysis, enzyme deactivation, centrifugation, supernatant taking and drying on the meat emulsion to obtain deer myocardial peptide; the protease used for enzymolysis is papain and flavourzyme, the mass ratio of feed liquid is 1:3-3.5, the enzymolysis time is 4-5 h, the papain and flavourzyme with the enzyme addition ratio of 4-4.5%2-2.5%are used for enzymolysis to obtain the deer myocardial peptide product with good flavor, the surface of the deer myocardial peptide is coated with the composite wall material, and the deer myocardial peptide with high oxidation resistance, good antibacterial property, high storage stability and low cost can be prepared by combining ultrasonic treatment and drying technology.

Inventors

  • ZHANG LULU
  • YU LI
  • XU CHENGYE
  • XU NING
  • LI QING
  • PI YUZHEN
  • GUAN MEIYU

Assignees

  • 北京同仁堂(辽宁)科技药业有限公司

Dates

Publication Date
20260505
Application Date
20260403

Claims (10)

  1. 1. A method for preparing deer myocardial peptide, which is characterized by comprising the following steps: (1) Thawing frozen deer heart, removing fascia, a tube and fat, cutting into pieces, cleaning, and mincing into meat emulsion; (2) Performing enzymolysis, enzyme deactivation, centrifugation, supernatant taking and drying on the meat emulsion to obtain deer myocardial peptide; the protease used for enzymolysis is papain and flavourzyme, the mass ratio of feed liquid is 1:3-3.5, and the enzymolysis time is 4-5 h.
  2. 2. The method for preparing deer myocardial peptide according to claim 1, wherein the addition ratio of papain to flavourzyme is 4-4.5%o and 2-2.5%o.
  3. 3. The method for preparing deer myocardial peptide according to claim 1, wherein the protein content is 3.83-3.94%, the protein recovery rate is 90.23-93.36%, and the molecular weight distribution rate of 202-3000 Da is 90.6-93.8% measured after enzymolysis.
  4. 4. The method for preparing deer myocardial peptide according to claim 1, wherein the drying mode is one of spray drying, freeze drying and low temperature vacuum belt drying.
  5. 5. The deer myocardial peptide is characterized by being prepared by the preparation method of any one of claims 1-4, wherein the peptide fragment sequence of the deer myocardial peptide comprises DWRPPRQ, WHEP, RHPYF and NGPDHWHE, DWRPPRQ with the amino acid sequence shown in SEQ ID NO 1, the amino acid sequence of WHEP shown in SEQ ID NO 2, the amino acid sequence of RHPYF shown in SEQ ID NO 3 and the amino acid sequence of NGPDHWHE shown in SEQ ID NO 4.
  6. 6. The use of deer myocardial peptide according to claim 5, wherein the deer myocardial peptide is mixed with the composite wall material and then subjected to ultrasonic treatment, the composite wall material is coated on the surface of the deer myocardial peptide, and the deer myocardial peptide microcapsule is formed after drying; The ratio of the core material to the composite wall material of the deer myocardial peptide microcapsule is 1:3-5, the ultrasonic treatment time is 30-60 min, the power is 125-175W, and the temperature is 40-60 ℃.
  7. 7. The use of deer myocardial peptide according to claim 6, wherein the composite wall material is resistant dextrin and chitosan derivatives.
  8. 8. The use of deer myocardial peptide according to claim 6, wherein the composite wall material is resistant dextrin, modified zein and chitosan derivatives.
  9. 9. The use of deer myocardial peptide according to claim 8, wherein the preparation method of the modified zein comprises the following steps: (1) Removing starch and decoloring corn protein powder by adopting alpha-amylase and acetone, leaching by using an ethanol solution with the volume fraction of 70%, centrifuging for 20-30 min under the condition of 3950-4100 r/min, taking supernatant, leaching by using ice water, taking precipitate, and freeze-drying to obtain zein; (2) Dissolving zein in an ethanol solution with the volume fraction of 70%, slowly adding the zein into a glucose solution with the mass fraction of 5%, stirring for reaction, and placing the mixture in a water bath with the temperature of 55-65 ℃ for ultrasonic treatment for 5-8min to prepare modified zein; the mass ratio of the glucose to the zein is 1:8-12, the stirring reaction time is 20-40 min, and the ultrasonic power is 400-500W.
  10. 10. The use of deer myocardial peptide according to claim 7 or 8, wherein the preparation method of the chitosan derivative comprises the steps of dissolving chitosan in acetic acid aqueous solution, magnetically stirring to become clear and transparent solution, then adding ascorbic acid and hydrogen peroxide, continuously stirring for 25-40 min under the protection of nitrogen, then adding rosmarinic acid, continuously reacting at room temperature for 10-12 h, introducing the obtained polymer solution into a dialysis bag after the reaction is completed, immersing into a glass container containing deionized water for dialysis, and finally freeze-drying to prepare the chitosan derivative; The molar ratio of the chitosan repeating unit to the rosmarinic acid is 1:1, the concentration of the ascorbic acid is 0.2-0.4 mmol/L, and the concentration of the hydrogen peroxide is 600-1000 mmol/L.

Description

Deer myocardial peptide and preparation method and application thereof Technical Field The invention belongs to the technical field of small molecular peptides, and particularly relates to deer myocardial peptide, and a preparation method and application thereof. Background The deer heart peptide is a deer heart small molecular peptide product, the influence of bitterness and astringency on the peptide product is larger, the peptide product is a main factor influencing the flavor of the deer heart small molecular peptide product, in addition, the peptide product is easy to be inactivated by the influence of light, heat, oxygen, acid-base environment or enzyme in the processing and storage processes, the deer heart peptide is wrapped in a microcapsule formed by a wall material through a microcapsule embedding technology to form a physical barrier so as to prevent the deer heart peptide from being directly contacted with the external environment, thus improving the stability and slow release performance of the deer heart peptide in the processing and storage processes, but the traditional embedding method comprises spray drying, an emulsifying method, a complex coacervation method and the like, has the problems of low embedding rate, high energy consumption, complex process and the like, is not suitable for industrial production, and the existing microcapsule wall materials are mostly used with soybean protein, whey protein or casein, but the wall materials are weak in oxidation resistance or do not have the oxidation resistance, and the oxidation resistance effect of the prepared deer heart peptide microcapsule in the storage processes is not ideal, and the application of products is influenced. Disclosure of Invention In order to solve the defects in the background art, the invention aims to provide the deer myocardial peptide, the preparation method and the application thereof, wherein papain and flavourzyme with the addition ratio of 4-4.5 per mill to 2-2.5 per mill are utilized for enzymolysis to obtain a deer myocardial peptide product with good flavour, and the deer myocardial peptide is prepared by coating a composite wall material on the surface of the deer myocardial peptide and combining an ultrasonic treatment and drying technology. The aim of the invention can be achieved by the following technical scheme: a method for preparing deer myocardial peptide, comprising the following steps: (1) Thawing frozen deer heart, removing fascia, a tube and fat, cutting into pieces, cleaning, and mincing into meat emulsion; (2) Performing enzymolysis, enzyme deactivation, centrifugation, supernatant taking and drying on the meat emulsion to obtain deer myocardial peptide; the protease used for enzymolysis is papain and flavourzyme, the mass ratio of feed liquid is 1:3-3.5, and the enzymolysis time is 4-5 h. Preferably, the addition ratio of the papain to the flavourzyme is 4-4.5 per mill and 2-2.5 per mill. Preferably, the protein content is 3.83-3.94%, the protein recovery rate is 90.23-93.36%, the molecular weight distribution rate of 202-3000 Da is 90.6-93.8%, and the average molecular weight is about 500Da. Preferably, the drying mode adopts one of spray drying, freeze drying and low-temperature vacuum belt drying. The deer myocardial peptide is prepared by the preparation method, wherein the peptide fragment sequence of the deer myocardial peptide comprises DWRPPRQ, WHEP, RHPYF and NGPDHWHE, DWRPPRQ, the amino acid sequence of WHEP is shown as SEQ ID NO 1, the amino acid sequence of WHEP is shown as SEQ ID NO 2, the amino acid sequence of RHPYF is shown as SEQ ID NO 3, and the amino acid sequence of NGPDHWHE is shown as SEQ ID NO 4. The application of the deer myocardial peptide comprises the steps of mixing the deer myocardial peptide with a composite wall material, performing ultrasonic treatment, coating the composite wall material on the surface of the deer myocardial peptide, and drying to form a deer myocardial peptide microcapsule; The ratio of the core material to the composite wall material of the deer myocardial peptide microcapsule is 1:3-5, the ultrasonic treatment time is 30-60 min, the power is 125-175W, and the temperature is 40-60 ℃. Preferably, the composite wall material is resistant dextrin and chitosan derivative. Preferably, the composite wall material is resistant dextrin, modified zein and chitosan derivative. Preferably, the preparation method of the modified zein comprises the following steps: (1) Removing starch and decoloring corn protein powder by adopting alpha-amylase and acetone, leaching by using an ethanol solution with the volume fraction of 70%, centrifuging for 20-30 min under the condition of 3950-4100 r/min, taking supernatant, leaching by using ice water, taking precipitate, and freeze-drying to obtain zein; (2) Dissolving zein in an ethanol solution with the volume fraction of 70%, slowly adding the zein into a glucose solution with the mass fraction of 5%, stirring