CN-121975903-A - Application of electrochemical active bacteria sensitive to polycyclic aromatic hydrocarbon pollutants in detection of water quality biotoxicity

CN121975903ACN 121975903 ACN121975903 ACN 121975903ACN-121975903-A

Abstract

The invention discloses an application of electrochemical active bacteria sensitive to polycyclic aromatic hydrocarbon pollutants in detecting water quality biotoxicity, which belongs to the technical field of water quality biotoxicity detection and is used for detecting the water quality biotoxicity of toxic pollutants according to the electrical signal inhibition rate of bacterial liquid of the electrochemical active bacteria, wherein the electrochemical active bacteria are azoospira, in particular to azoospira bassiana. The novel water quality biotoxicity detection technology based on the electrochemical active bacteria can directly convert toxicity into an electric signal, is hopeful to improve the sensitivity of measuring the biotoxicity of the polycyclic aromatic hydrocarbon pollutant, overcomes the application bottleneck of low sensitivity, and provides technical support for biotoxicity monitoring work in China.

Inventors

YI YUE
LI JIA
FENG YUXIN
LUO AIQIN

Assignees

北京理工大学

Dates

Publication Date: 20260505
Application Date: 20260206
Priority Date: 20251211

Claims (7)

1. The application of electrochemical active bacteria sensitive to polycyclic aromatic hydrocarbon pollutants in detecting water quality biotoxicity is characterized in that the water quality biotoxicity of toxic pollutants is measured according to the electrical signal inhibition rate of bacterial liquid of the electrochemical active bacteria; The toxic pollutant is polycyclic aromatic hydrocarbon pollutant; the electrochemical active bacteria are azospirillum, in particular to azospirillum bassinense.
2. The use according to claim 1, wherein the preparation method of the bacterial liquid of the electrochemical active bacteria comprises the following steps: And culturing the electrochemical active bacteria to a stationary phase, centrifuging, and re-suspending bacterial precipitation by using a solution to be detected or pure water to obtain bacterial liquid of the electrochemical active bacteria.
3. The use of claim 1, wherein the polycyclic aromatic hydrocarbon contaminant comprises one of benzanthracene, benzopyrene, benzophenanthrene, anthracene, benzo [ b ] fluoranthene.
4. The use according to claim 1, wherein the electrical signal rejection rate is calculated by: electric signal inhibition ratio cr= (C 0 -C）/C 0 ×100%; Wherein, C is an electric signal of bacterial liquid of electrochemical active bacteria obtained by using a solution to be detected to resuspend bacterial precipitation; C 0 is an electrical signal of a bacterial liquid of the electrochemically active bacteria obtained by re-suspending bacterial precipitation using pure water.
5. The use according to claim 4, wherein the method of detecting the electrical signal comprises the steps of: Adding NaCl solution into the bacterial liquid of the electrochemical active bacteria, exposing at Wen Zhendang deg.C, adding potassium ferricyanide solution, high-temperature oscillating reaction, centrifuging after the reaction, adding the supernatant into screen printing electrode, and testing current by using a chronoamperometry.
6. The use according to claim 5, wherein the high temperature shock exposure is at 50 ℃, for 2 hours, and the vortex shock speed is 200rpm.
7. The use according to claim 6, wherein the high temperature shaking reaction is carried out at a temperature of 50 ℃ for a time of 1h and a shaking speed of 200rpm.

Description

Application of electrochemical active bacteria sensitive to polycyclic aromatic hydrocarbon pollutants in detection of water quality biotoxicity Technical Field The invention belongs to the technical field of water quality biotoxicity detection, and particularly relates to application of electrochemical active bacteria sensitive to polycyclic aromatic hydrocarbon pollutants in detection of water quality biotoxicity. Background Currently, the detection of toxic and harmful pollutants is mainly automatic and manually assisted fusion monitoring, and only manual analysis can detect toxic and harmful pollutants. However, the manual analysis has limited types of pollutants, the frequency is about 1 time per month, and the early warning of sudden water pollution is difficult. In order to improve the early warning capability, the biotoxicity index is used for expanding the water quality automatic monitoring project. Different from analyzing the specific pollutant types and contents, the biotoxicity index comprehensively reflects the toxic effect of pollutants by analyzing the inhibition effect of all toxic pollutants in the water body on biological metabolism. The determination of biotoxicity requires the test organism, the most commonly used test organism being a luminescent bacterium. The luminescent bacteria are able to respond to toxic contaminants in a broad spectrum and in real time, for a period of only 15 minutes. However, the chromaticity and turbidity of the water body directly influence the light intensity of the luminous bacteria, so that the turbid surface water and colored wastewater are easy to generate biological toxicity false positive. Aiming at the defect of determining the biotoxicity of water quality by using luminescent bacteria, it is proposed to use electrochemical active bacteria to replace luminescent bacteria in the world to determine the biotoxicity of water quality. Electrochemically active bacteria are bacteria with unique energy metabolism patterns that are capable of transporting electrons generated by respiration to the outside of the cell. Under normal physiological conditions, electrochemically active bacteria are capable of producing stable electrical signals. When the electrochemical active bacteria are exposed to toxic water, the metabolic activity of the electrochemical active bacteria is reduced, and the electrical signal is reduced, so that the water biotoxicity can be judged by analyzing the electrical signal of the electrochemical active bacteria. The electrochemically active bacteria and the luminescent bacteria belong to the same genus of prokaryotes and can respond to toxic pollutants rapidly. Meanwhile, compared with luminescent bacteria, the electrochemical active bacteria are mainly derived from fresh water environment, and the salinity is not required to be preset. In addition, the electrochemical active bacteria can directly generate electric signals, the detection process is not interfered by the chromaticity and turbidity of the water body, and the measurement result has higher accuracy, so that the electrochemical active bacteria can be used as the only tested organism which can directly reflect the biotoxicity of the water quality by the electric signals, and become a research hot spot at home and abroad. Currently, a key technical bottleneck in determining biotoxicity using electrochemically active bacteria is lower sensitivity. The basis for the response of electrochemically active bacteria to poisoning contaminants is the sensitivity of the bacteria. In reported studies, only pure culture electrochemically active bacterial model strains, including SHEWANELLA ONEIDENSISMR-1 and SHEWANELLA LOIHICAPV-4, were used to determine water biotoxicity. However, few studies on electrochemically active bacteria sensitive to poisoning contaminants have been performed, and particularly, electrochemically active bacteria sensitive to polycyclic aromatic hydrocarbon contaminants have not been reported. Polycyclic aromatic hydrocarbons are a widely occurring class of persistent organic pollutants in the environment, mainly produced by incomplete combustion of carbon-containing organic matter. Due to its high stability, high fat solubility and ability to be enriched by the food chain, polycyclic aromatic hydrocarbons pose a serious threat to the ecosystem and human health, and the international cancer research institute has listed some polycyclic aromatic hydrocarbons (e.g. benzo [ a ] pyrene) as well-defined human carcinogens. Therefore, how to provide an electrochemical active bacteria sensitive to polycyclic aromatic hydrocarbon pollutants, and to improve the sensitivity of determining biotoxicity are technical problems that need to be solved by those skilled in the art. Disclosure of Invention In order to solve the technical problems, the invention provides application of electrochemical active bacteria sensitive to polycyclic aromatic hydrocarbon pollutants in detecting w