CN-121975950-A - Application of PIEZO1 gene mutation in selection of lambing number of Macheng black goats

Abstract

The invention discloses application of SNP markers affecting goat lambing number. The PIEZO1 gene marked on goat (Capra hircus) 18 chromosome has specific SNP marked site of A/C base mutation at 236bp of SEQ ID NO. 1 in the sequence table, and the CC genotype and the lambing number of AC genotype goat individuals are obviously higher than those of AA genotype individuals. According to the invention, macheng black goats are taken as research objects, DNA sequences of 33 rd exons of PIEZO1 genes are amplified by PCR, SNP variation sites exist in the sequences, the influence of the site polymorphism on the lambing number of the goats is analyzed, and accordingly, the seed reserving of Macheng black goats is carried out, so that the method can be used for improving the lambing number of Macheng black goats, accelerating the cultivation of new varieties (strains) taking Macheng black goats as breeding materials, and providing marking resources for marking auxiliary selection breeding of the lambing number characters of goats.

Inventors

• LIU GUIQIONG
• JIANG XUNPING
• LIU JUNCHENG
• Tie Zhuoying
• LI JIACHENG
• DAI HONGJIE
• LI LONG

Assignees

• 华中农业大学
• 湖北都宏生态农业发展有限公司

Dates

Publication Date

20260505

Application Date

20260316

Claims (2)

1. 1. A method for selecting a high lambing goat based on a molecular marker associated with the lambing number of the goat, which molecular marker is located in the nucleotide sequence SEQ ID NO:1, wherein the 236bp site base of the sequence has a C/A polymorphism, and the lambing number of a CC genotype and an AC genotype goat individual of the molecular marker site is significantly higher than that of an AA genotype goat individual, which method comprises the following steps: (1) Extracting goat genome DNA; (2) Performing PCR amplification by using two specific primers to obtain 690bp amplification products, wherein the sequences of the two specific primers are SEQ ID NO.2 and SEQ ID NO. 3; (3) Sequencing the PCR amplification product to obtain a sequencing result; (4) Determining the genotype of the goat individual to be detected in the molecular marker according to the sequencing result; (5) Selecting goat individuals of the CC genotypes of the molecular markers for seed reservation; The goat is Macheng black goats.
2. 2. Use of the molecular marker of claim 1 for screening black goats with high lambing number Macheng, selecting CC genotype goats individuals with said molecular marker for seed retention.

Description

Application of PIEZO1 gene mutation in selection of lambing number of Macheng black goats Technical Field The invention belongs to the technical field of animal molecular breeding, and relates to application of SNP markers related to Macheng black goats lambing number traits in Macheng black goats breeding. Background The goat lambing number is a key character affecting the economic benefit of breeding, the hereditary power is low, the hereditary progress obtained by the traditional breeding method is slow and the selection effect is unstable, the early selection can be realized by molecular marker assisted selection, and the selection efficiency and the accuracy are high. The number of lambs has major genes, and the presently known number of lambs major genes are mainly focused on hormone and follicular development pathways regulated by TGF-beta signaling pathways (e.g., BMPR1B, GDF 9) and steroid hormone metabolic pathways (e.g., ESR 1). The PIEZO family is a key mechanical sensitive cation channel family in mammals, core members are PIEZO1 and PIEZO2, and the PIEZO1 and the PIEZO2 are provided with unique three-blade propeller-shaped trimer structures, and the structures can realize the conversion from mechanical stimulation to electrochemical signals, so that the wide mechanical force transduction process of an organism is mediated. PIEZO2 is mainly concentrated in sensory neurons and related tissues, and core functions are concentrated in somatosensory regulation and control, including signal transduction of touch sense, proprioception and mechanical pain sense, and simultaneously participate in respiratory tract stretch sensing, bladder filling monitoring and construction of blood pressure reflex arcs. PIEZO1 takes mechanical force transduction as a core function to participate in a plurality of physiological processes, and in a cardiovascular system, blood tension, blood pressure steady state and angiogenesis are regulated and controlled by sensing blood flow shearing force, so that tissue blood supply is ensured. In the reproductive system, PIEZO1 is closely related to animal reproductive performance, can regulate and control proliferation and differentiation of placenta trophoblast cells, can maintain uterine blood supply during pregnancy by regulating and controlling vasodilation of uterine arteries, and can influence follicular dominance selection and ovulation efficiency by participating in perception of mechanical microenvironment during follicular development. Thus PIEZO1 performs reproductive regulation from dominant follicular selection, ovulation, placental trophoblast fusion, uterine blood supply during gestation and even delivery initiation, which regulates physiological processes of reproduction which may affect lambing numbers. The invention discovers that a mutation site of the PIEZO1 gene is obviously related to the lambing number of goats, and provides a marker resource for molecular marker-assisted selective breeding of Macheng black goats with the lambing number character. Disclosure of Invention The invention provides a method for selecting goat seed retention according to the genotype of the marker loci. According to the embodiment of the invention, the SNP marker is positioned at the 236 th base of the nucleotide sequence shown in SEQ ID NO. 1, and the CC genotype and the lambing number of the AC genotype goat individual of the SNP marker locus are obviously higher than those of the AA genotype goat individual. By detecting the SNP marker of Macheng black goats, the lambing number of goat individuals can be estimated according to the genotype of the SNP marker locus. Therefore, the SNP marker is closely related to the lambing number character of Macheng black goats, can be effectively used for auxiliary selective breeding of the molecular marker of Macheng black goats, and further can select goat breeding materials according to actual breeding requirements, so that goats with a large lambing number can be accurately and efficiently bred, and the efficiency and accuracy of selection in the breeding process are improved. The invention provides a primer pair for detecting the SNP marker. According to an embodiment of the present invention, the primer has the nucleotide sequences shown in SEQ ID NO. 2 and SEQ ID NO. 3 for detecting the SNP marker. According to the embodiment of the invention, the primer pair can be used for effectively amplifying the DNA fragment of the SNP marker related to the lambing number character of the goat to be detected, the SNP marker can be effectively detected through sequencing, the genotype of the goat individual to be detected at the SNP marker locus is determined, and the lambing number of the goat to be detected can be effectively predicted. Specifically, the number of the lambings of individuals with the CC genotype and the AC genotype of the SNP marker locus is obviously higher than that of individuals with the AA genotype, which shows that the C