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CN-121975952-A - SNP molecular marker related to grass carp specific growth rate and application thereof

CN121975952ACN 121975952 ACN121975952 ACN 121975952ACN-121975952-A

Abstract

The invention belongs to the field of aquatic animal molecular markers and aquatic genetic breeding, and particularly discloses an SNP marker remarkably related to a grass carp Specific Growth Rate (SGR) and application thereof. The SNP molecular marker is shown as follows, 1) is positioned at 31625913 th position of chromosome 13 of grass carp, polymorphic base is C/T, 2) is positioned at 31648914 th position of chromosome 13 of grass carp, and polymorphic base is G/A. The invention also discloses application of the SNP molecular marker, the amplification primer or the kit in breeding grass carp with high specific growth rate. The SNP molecular marker can be used for early breeding of grass carp with rapid growth potential, effectively avoids the limitation of relying on the later stage phenotype measurement of breeding in traditional breeding, obviously shortens the breeding period, improves the breeding efficiency, reduces the breeding cost, and has important application value for improving the production performance of grass carp, shortening the breeding period and improving the production benefit.

Inventors

  • XU XIAOYAN
  • Han Caitou
  • LI JIALE
  • Gui lang
  • SHEN YUBANG

Assignees

  • 上海海洋大学

Dates

Publication Date
20260505
Application Date
20260327

Claims (8)

  1. 1. SNP molecular markers related to grass carp specific growth rate, characterized in that the SNP molecular markers are 1) or 2) as follows: 1) The polymorphic base is C/T at 31625913 th position of chromosome 13 of grass carp; 2) The polymorphic base is G/A at 31648914 th chromosome 13 of grass carp.
  2. 2. Amplification primers for detecting SNP molecular markers associated with a specific growth rate of grass carp according to claim 1, characterized by comprising a primer pair capable of detecting one or both of the two SNP molecular markers.
  3. 3. The amplification primer of claim 2, comprising: A pair of primers shown as SEQ ID NO. 1 and SEQ ID NO. 2 for detecting the molecular marker 1), and/or, A pair of primers shown in SEQ ID NO. 3 and SEQ ID NO. 4 for detecting the molecular marker 2).
  4. 4. A method for breeding grass carp with high specific growth rate, which is characterized by comprising the following steps: step one, extracting individual fin DNA of grass carp to be detected; Step two, using the genome DNA in the step one as a template, and performing PCR amplification by using the amplification primer according to claim 3; Step three, sequencing the amplification product obtained in the step two, determining the genotype of the SNP molecular marker of the grass carp individual to be detected, and determining whether the grass carp has the genetic potential of high specific growth rate through genotype analysis.
  5. 5. The method for breeding grass carp with high specific growth rate according to claim 4, wherein in the third step, when PCR amplification is performed by using a pair of primers shown as SEQ ID NO.1 and SEQ ID NO. 2, a polymorphic base at 31625913 th position of chromosome 13 of grass carp is detected, and when the genotype of the locus is CC, the grass carp individual is judged to have higher specific growth rate genetic potential.
  6. 6. The method for breeding grass carp with high specific growth rate according to claim 4, wherein in the third step, when PCR amplification is performed by using a pair of primers shown as SEQ ID NO.3 and SEQ ID NO. 4, polymorphic nucleotide at 31648914 of chromosome 13 of grass carp is detected, and when genotype of the locus is GG, the grass carp individual is judged to have higher specific growth rate genetic potential.
  7. 7. A kit comprising amplification primers capable of detecting SNP molecular markers associated with a particular growth rate of grass carp as defined in claim 1.
  8. 8. Use of the SNP molecular marker of claim 1, the amplification primer of claim 2 or 3 or the kit of claim 7 for breeding grass carp with high specific growth rate.

Description

SNP molecular marker related to grass carp specific growth rate and application thereof Technical Field The invention relates to the technical field of aquatic animal molecular markers and aquatic genetic breeding, and relates to SNP molecular markers related to grass carp Specific Growth Rate (SGR) and application thereof. Background Grass carp (Ctenopharyngodon idella) is one of the most important freshwater aquaculture economic fishes in China, and the growth speed is a key index for influencing the aquaculture benefit. Wherein, the specific growth rate (Specific Growth Rate, SGR) is used as a core parameter for measuring the growth performance, and directly determines the cultivation period and the production efficiency. However, grass carp has biological characteristics of late sexual maturity (usually 4-5 years), long breeding cycle, so that the traditional phenotype-based breeding method is long in time consumption and low in efficiency, and the cultivation process of excellent grass carp varieties is severely limited. Therefore, the development of molecular markers closely related to Specific Growth Rate (SGR) and the realization of early and efficient molecular marker assisted selection have become urgent demands in the grass carp genetic breeding field. Molecular markers are genetic markers based on nucleotide sequence variations in the genetic material between individuals, directly reflecting genetic polymorphisms at the DNA level. Among them, single nucleotide polymorphisms (Single nucleotide polymorphism, SNPs) are widely used for molecular breeding of animals and plants because of the largest number and the largest distribution in the genome as third generation molecular markers. Molecular marker assisted breeding is a breeding technology for selecting target traits by detecting molecular markers by utilizing the characteristic that the molecular markers are closely linked with genes for controlling the target traits. However, there is currently a lack of molecular markers for molecular marker-assisted breeding of grass carp growth traits, which restricts improvement of grass carp growth traits. Disclosure of Invention The invention aims to improve SNP molecular markers related to grass carp Specific Growth Rate (SGR) and application thereof so as to solve the problems. In order to achieve the above purpose, the invention adopts the following specific technical scheme: in a first aspect, the present invention provides a SNP molecular marker associated with a particular growth rate (SGR) of grass carp, said SNP molecular marker being 1) or 2) as follows: 1) The polymorphism site base is C/T at 31625913 th chromosome of grass carp No. 13, and is called molecular marker SNP Chr13:31625913 in the text, and the polymorphism site genotype is CC and corresponds to high specific growth rate; 2) The polymorphism site base is G/A at 31648914 th chromosome of grass carp No. 13, and is called molecular marker SNP Chr13:31648914, and the polymorphism site genotype is GG, which corresponds to high specific growth rate. In a second aspect, the invention provides amplification primers for detecting said SNP molecular markers associated with Specific Growth Rate (SGR) of grass carp, comprising a primer pair capable of detecting one or both of the two SNP molecular markers. Preferably, the amplification primers comprise one or more of the following pairs: a pair of primer pairs shown as SEQ ID NO. 1 and SEQ ID NO.2 can amplify the molecular marker SNP, namely, chr13:31625913; a pair of primer pairs shown as SEQ ID NO. 3 and SEQ ID NO. 4 can amplify the molecular marker SNP, namely, chr13:31648914; in a third aspect, the present invention provides a method of breeding grass carp having a high Specific Growth Rate (SGR), comprising the steps of: step one, extracting individual fin DNA of grass carp to be detected; And step two, taking the genome DNA in the step one as a template, and carrying out PCR amplification by using the amplification primer. Step three, sequencing analysis is carried out on the amplified product obtained in the step two, the genotype of the SNP molecular marker of the grass carp individual to be detected is determined, and whether the grass carp has the genetic potential of high Specific Growth Rate (SGR) is determined through genotype analysis. Preferably, in the third step, when PCR amplification is carried out by using a pair of primer pairs shown as SEQ ID NO. 1 and SEQ ID NO. 2, polymorphic base at 31625913 th position of chromosome 13 of grass carp is detected, and grass carp with genotype CC (base sequence shown as SEQ ID NO. 5) has the potential of excellent high Specific Growth Rate (SGR). Preferably, in the third step, when PCR amplification is carried out by using a pair of primer pairs shown as SEQ ID NO. 3 and SEQ ID NO. 4, polymorphic base at 31648914 th position of chromosome 13 is detected, and grass carp with genotype GG (the base sequence shown as SEQ ID NO. 6) has the po