CN-121975955-A - SNP molecular marker associated with weight under turbot fermented soybean meal feeding and application thereof

Abstract

The invention relates to a SNP molecular marker related to turbot weight under fermented soybean meal feeding and application thereof, belonging to the technical field of molecular markers, the invention utilizes whole genome related analysis to analyze turbot weight traits to obtain SNP molecular markers obviously related to the weight traits under fermented soybean meal feeding, the method comprises at least one of 6 SNP loci, can be applied to turbot molecular assisted breeding, can greatly shorten the breeding period, reduce the dependence on phenotype characteristics, improve the selective breeding efficiency and have good application prospect by screening on a genome level by the breeding method disclosed by the invention.

Inventors

• SUN ZHIBIN
• MA AIJUN
• YANG JINGKUN
• WANG XINAN
• LIU ZHIFENG
• HUANG ZHIHUI
• GAO YUNYI
• MA CONG
• WANG YILIN

Assignees

• 中国水产科学研究院黄海水产研究所

Dates

Publication Date

20260505

Application Date

20260408

Claims (6)

1. 1. A turbot fermented soybean meal feeding weight-related SNP molecular marker is characterized in that the molecular marker is at least one of the following, the nucleotide sequence of the molecular marker is shown as SEQ ID NO.1, the 101 st position of the molecular marker is provided with a turbot weight-related SNP locus, the allele of the locus is A or C, and the molecular marker is denoted as chr12:4477381; The nucleotide sequence of the molecular marker is shown as SEQ ID NO.5, the 101 st position of the molecular marker is provided with a SNP locus related to turbot body weight, the allele of the locus is A or T, and the molecular marker is marked as chr12:4477315; The nucleotide sequence of the molecular marker is shown as SEQ ID NO.9, the 101 st position of the molecular marker is provided with a SNP locus related to turbot body weight, the allele of the locus is G or T, and the molecular marker is marked as chr12:4630675; The nucleotide sequence of the molecular marker is shown as SEQ ID NO.13, and the 101 st position of the molecular marker is provided with a SNP locus related to turbot body weight, and the allele of the locus is T or G, wherein the molecular marker is marked as chr1:9125902; The nucleotide sequence of the molecular marker is shown as SEQ ID NO.17, and the 101 st position of the molecular marker is provided with a SNP locus related to turbot body weight, and the allele of the locus is T or G, wherein the molecular marker is marked as chr1:9114554; the nucleotide sequence of the molecular marker is shown as SEQ ID NO.21, the 101 st position of the molecular marker is provided with a SNP locus related to turbot body weight, the allele of the locus is G or A, and the molecular marker is marked as chr1:9271275.
2. 2. The SNP molecular marker combination is characterized in that the SNP molecular marker combination is one of chr1:9125902-TG and chr1:9114554-TG; chr1:9114554-TG and chr1:9271275-GA; chr12:4477381-AA, chr1:9125902-TG and chr1:9114554-TG; the nucleotide sequences of the SNP molecular markers are as described in claim 1, and the SNP molecular markers have the advantage of gene type on the SNP molecular markers, wherein the nucleotide sequences are chr12:4477381-AA, chr12:4477315-AA and chr1:9114554-TG, chr12:4477381-AA, chr12:4477315-AA, chr1:9125902-TG and chr1:9125902-TG, chr12: 9125902-AA, chr1:9125902-TG and chr1: 9125902-GA, chr12: 9125902-AA, chr12: 9125902-TT, chr1:9125902-TG and chr1: 9125902-GA.
3. 3. The primer for amplifying the SNP molecular marker is characterized in that the primer can amplify the SNP molecular marker.
4. 4. Use of the SNP molecular marker according to claim 1 in turbot body weight trait breeding, characterized in that the use is by selecting individuals with dominant genotypes as body weight trait breeding parents.
5. 5. Use of a combination of molecular markers according to claim 2 in the breeding of turbot body weight traits by selecting as body weight trait breeding parents individuals having a dominant genotype at the corresponding SNP locus in the combination of molecular markers.
6. 6. The application of the primer in turbot body weight trait breeding, which is characterized in that the primer is used for amplifying turbot individuals, and individuals with dominant genotypes in the molecular markers are screened as body weight trait breeding parents.

Description

SNP molecular marker associated with weight under turbot fermented soybean meal feeding and application thereof Technical Field The invention belongs to the technical field of molecular markers, and particularly relates to a SNP molecular marker associated with weight under the condition of turbot fermented soybean meal feeding and application thereof. Background Turbot (Scophthalmus maximus) is an important economic fish for cold water mariculture in northern China, and has high commercial value due to high growth speed and delicious meat quality. In the traditional cultivation mode, the growth of turbots is highly dependent on ration with high fish meal content. However, with the shortage of global fish meal resources and rising prices, the development of efficient, low cost vegetable protein sources (e.g., soybean meal) to replace fish meal has become a necessary trend in the aquaculture feed industry. Research shows that the anti-nutritional factors in the common soybean meal can induce turbots to have physiological problems such as intestinal inflammation, reduced digestibility and the like. Although the fermented soybean meal remarkably reduces the content of anti-nutritional factors and improves the palatability through microbial fermentation, in actual production, turbot individuals with different genetic backgrounds are found to have remarkable differences in the utilization capacity of the fermented soybean meal daily ration. This genotype interaction with nutritional metabolism results in significant differentiation of individuals' weight gain in the population under low fish meal (high fermented soybean meal) feeding conditions. The traditional breeding method mainly depends on phenotype selection, is greatly influenced by environment, has long period, and is difficult to accurately screen out good varieties with high-efficiency transformation capability on novel feeds. Although turbot growth trait-associated SNPs (single nucleotide polymorphisms) have been reported in recent years, the contribution rate of genetic variation explained by single SNP sites is generally limited. Haplotypes (Haplotype) consist of a set of SNP sites that are closely linked in physical position, and compared with single SNP, haplotypes can provide more abundant genetic information, and have higher polymorphism and genetic stability. Under the background of fermented soybean meal feeding, haplotypes obviously related to body weight traits are excavated and applied to early molecular marker assisted breeding (MAS), and the method has important economic value and scientific research significance for cultivating novel turbot varieties with excellent plant protein fish meal feed tolerance and growth traits. Disclosure of Invention Aiming at the technical problems, the invention provides a group of SNP molecular markers in a fermented soybean meal feeding background, which can be used for molecular assisted breeding of new lines of turbot body weight traits. The invention aims to provide a SNP molecular marker related to the body weight of turbot under the condition of fermented soybean meal feeding, wherein the molecular marker is at least one of the following nucleotide sequences shown in SEQ ID NO.1, the 101 st position of the molecular marker is provided with a SNP locus related to the body weight of the turbot, the allele of the locus is A or C, and the molecular marker is marked as chr12:4477381; The nucleotide sequence of the molecular marker is shown as SEQ ID NO.5, the 101 st position of the molecular marker is provided with a SNP locus related to turbot body weight, the allele of the locus is A or T, and the molecular marker is marked as chr12:4477315; The nucleotide sequence of the molecular marker is shown as SEQ ID NO.9, the 101 st position of the molecular marker is provided with a SNP locus related to turbot body weight, the allele of the locus is G or T, and the molecular marker is marked as chr12:4630675; The nucleotide sequence of the molecular marker is shown as SEQ ID NO.13, and the 101 st position of the molecular marker is provided with a SNP locus related to turbot body weight, and the allele of the locus is T or G, wherein the molecular marker is marked as chr1:9125902; The nucleotide sequence of the molecular marker is shown as SEQ ID NO.17, and the 101 st position of the molecular marker is provided with a SNP locus related to turbot body weight, and the allele of the locus is T or G, wherein the molecular marker is marked as chr1:9114554; the nucleotide sequence of the molecular marker is shown as SEQ ID NO.21, the 101 st position of the molecular marker is provided with a SNP locus related to turbot body weight, the allele of the locus is G or A, and the molecular marker is marked as chr1:9271275. The second purpose of the invention is to provide a group of SNP molecular marker combinations related to the weight of turbot fermented soybean meal fed, wherein the SNP molecular marker combinations a