CN-121975974-A - SV (SV) marker closely linked with resistance to brown spot of eggplant and application thereof

Abstract

The invention belongs to the field of molecular biology, and particularly relates to an SV marker closely linked with eggplant brown spot resistance and application thereof, wherein the SV marker is a 119 bp deletion sequence of Chr11:98755912, the nucleotide sequence of the SV marker is shown as SEQ ID NO.1, and a theoretical basis and an effective molecular marker can be provided for eggplant brown spot resistance molecular breeding by developing a specific molecular marker of SV 98755912.

Inventors

• YOU QIAN
• Jiang Yaolan
• ZHAO WEI
• SUN BAOJUAN
• LI TAO

Assignees

• 广东省农业科学院蔬菜研究所

Dates

Publication Date

20260505

Application Date

20260318

Claims (8)

1. 1. An SV marker closely linked to resistance to eggplant brown spot is characterized in that the nucleotide sequence of the SV marker is shown as SEQ ID NO. 1.
2. 2. A primer for detecting the SV mark as claimed in claim 1, wherein the nucleotide sequences of the primer are shown in SEQ ID NO.2 and SEQ ID NO. 3.
3. 3. A kit for detecting the SV marker of claim 1 comprising the primer of claim 2.
4. 4. A screening method of eggplant brown spot resistant materials is characterized by comprising the steps of extracting DNA of eggplant samples, amplifying the extracted DNA of the eggplant samples by using the primer according to claim 2 or the kit according to claim 3, and performing electrophoresis detection, wherein if amplified fragments exist, the eggplant samples are brown spot resistant materials, and if no amplified fragments are brown spot resistant materials.
5. 5. The method for screening eggplant brown spot resistant material according to claim 4, wherein the amplification reaction procedure is 94 ℃ pre-denaturation for 3min, 94 ℃ denaturation for 30 s,56 ℃ annealing for 30 s,72 ℃ extension for 30 s, 31 cycles, 72 ℃ extension for 5min.
6. 6. Use of the SV marker of claim 1 in screening eggplant brown streak resistant material.
7. 7. Use of the SV marker of claim 1 in the detection of eggplant brown streak.
8. 8. Use of the primer of claim 2 or the kit of claim 3 for the preparation of a reagent for detecting eggplant brown streak resistant material.

Description

SV (SV) marker closely linked with resistance to brown spot of eggplant and application thereof Technical Field The invention belongs to the field of molecular biology, and particularly relates to an SV (SV) marker closely linked with resistance to eggplant brown spot and application thereof. Background Brown spot of eggplant is a global fungal disease caused by brown spot germ (Phomopsis vexans) infection, can cause large-area yield reduction of eggplant, is one of destructive diseases which seriously affect quality and yield of eggplant, and seriously hinders development of eggplant industry. Eggplant brown spot resistance is a complex trait controlled by multiple genes. However, the existing eggplant brown spot resistant varieties at home and abroad are lack, and the research reports on the aspects of brown spot resistance inheritance, resistance gene positioning, linkage marking, disease resistance molecular mechanism and the like are less. Therefore, the development of the marker and the gene closely linked to brown spot resistance, and the breeding of excellent disease-resistant varieties by utilizing a molecular breeding means are the most effective measures for preventing brown spot. The application utilizes forward genetics means (GWAS positioning) to locate eggplant evm.model.Chr11.2544 as a brown spot resistance candidate gene, and performs research of related analysis and molecular marker development, thereby laying a foundation for eggplant brown spot resistance molecular marker assisted selection breeding. Disclosure of Invention Aiming at the defects of the prior art, the invention aims to provide an SV marker closely linked with eggplant brown spot resistance and application thereof, lays a foundation for the assisted selective breeding of eggplant brown spot resistance molecular markers, and improves breeding efficiency. In order to solve the problems, the invention adopts the following technical scheme: In a first aspect, the invention provides an SV marker closely linked to resistance to eggplant brown spot, wherein the nucleotide sequence of the SV marker is shown in SEQ ID NO. 1. In a second aspect, the invention provides a primer for detecting the SV mark, which is characterized in that the nucleotide sequence of the primer is shown as SEQ ID NO.2 and SEQ ID NO. 3. In a third aspect, the present invention provides a kit for detecting the SV marker, which is characterized by comprising the primer. In a fourth aspect, the invention provides a screening method of eggplant brown spot resistant materials, which comprises the steps of extracting DNA of eggplant samples, amplifying the extracted DNA of the eggplant samples by adopting the primer or the kit, and carrying out electrophoresis detection, wherein if amplified fragments exist, the eggplant samples are brown spot resistant materials, and if no amplified fragments are brown spot resistant materials. Further, the amplification reaction procedure was 94℃pre-denaturation for 3min, 94℃denaturation for 30s, 56℃annealing for 30s, 72℃extension for 30s, 31 cycles, and 72℃extension for 5min. In a fifth aspect, the invention provides the use of an SV marker as described in the screening of eggplant brown streak resistant material. In a sixth aspect, the invention provides the use of the SV marker in detection of brown streaks in eggplants. In a seventh aspect, the invention provides an application of the primer or the kit in preparing a reagent for detecting eggplant brown spot resistance materials. The invention has the beneficial effects that based on positioning the eggplant brown spot resistance linkage site by utilizing the GWAS analysis of various variations, the eggplant brown spot resistance candidate gene evm.model.Chr11.2544 is obtained through screening by bioinformatics analysis such as genome sequence analysis, the high linkage between the SV98755912 molecular marker and the resistance is determined, the specific molecular marker of the SV98755912 is developed, and a theoretical basis and an effective molecular marker can be provided for eggplant brown spot resistance molecular breeding. Drawings FIG. 1 is a map of SNPs-GWAS-localized eggplant brown spot resistance-related loci based on the values of the multiple-year, multiple-spot phenotype BLUE. FIG. 2 is a plot of InDel-GWAS-localized eggplant brown spot resistance-related sites based on years of multipoint phenotype BLUE values. FIG. 3 is a map of SV-GWAS localized eggplant brown spot resistance-related sites based on years of multipoint phenotype BLUE values. FIG. 4 is a haplotype map of eggplant brown streak resistance candidate genes. FIG. 5 is a graph showing the result of electrophoresis detection of 66 parts of eggplant germplasm resources by using SV98755912 molecular markers. Detailed Description The present invention will be described in further detail with reference to specific examples. It should be noted that these examples are only for illustrating the present in