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CN-121975977-A - Nucleic acid molecule combination of pathogen standard and application thereof

CN121975977ACN 121975977 ACN121975977 ACN 121975977ACN-121975977-A

Abstract

The invention belongs to the technical field of sequencing, and particularly relates to a nucleic acid molecule combination of pathogen standard substances and application thereof. The nucleic acid molecule combination of the pathogen standard can monitor multiple pathogens simultaneously, and can avoid sample pollution to generate false positive results by means of letter rejection, so that the problems that the existing pathogen standard can not completely monitor the detection level of a product to a target pathogen by only using one or more known pathogens are solved, but the type of PTC pathogen is easily polluted when the type of the PTC pathogen is increased.

Inventors

  • REN HAN
  • LIU JIUCHENG
  • WANG MINGFEI
  • LI QINGFEI
  • LIU JIAHUI

Assignees

  • 吉因加生物医学科技(绍兴)有限公司
  • 苏州吉因加生物医学工程有限公司
  • 深圳吉因加生物科技有限公司

Dates

Publication Date
20260505
Application Date
20260115

Claims (10)

  1. 1. Nucleic acid molecule combinations of pathogen standard comprising at least two of rabies virus, rhinovirus type A, staphylococcus cephalus, bordetella pertussis, enterovirus D, or The pathogen includes Cronobacter sakazakii, candida albicans, enterobacter cloacae, candida glabrata, influenza C virus, staphylococcus haemolyticus, human respiratory virus type 3, norovirus GII, severe acute respiratory syndrome related coronavirus, acinetobacter baumannii, enterococcus faecalis, adenovirus type 21, hanchen virus, human immunodeficiency virus type 1, measles virus, streptococcus pyogenes, vibrio vulnificus, corynebacterium diphtheriae, parvovirus B19, campylobacter foetus, enterococcus faecium, streptococcus agalactiae, respiratory syncytial virus, bacillus anthracis, clostridium perfringens, mycoplasma pneumoniae, yersinia enterocolitica, rubella virus, Coronavirus of the middle east respiratory syndrome, poliovirus type 1, human mumps virus type 2, coronavirus HCoV-OC43, cryptosporidium hominis, pr Luo Waci g (Pr) rickettsia, gettryococcus, shigella, staphylococcus Wona, rhinovirus type B, zika virus, human coronavirus HKU1, nocardia astrovirus, enterovirus C, aspergillus flavus, human herpesvirus type 3, human herpesvirus type 5, west Nile virus, enterovirus, influenza B virus, japanese blood fluke, rotavirus group A, clostridium difficile, mycobacterium tuberculosis, yersinia pseudotuberculosis, phagostimulant, Chlamydia psittaci, listeria monocytogenes, cryptococcus neoformans, aspergillus terreus, acinetobacter calcoaceticus, human respiratory tract virus type 1, rickettsia, klebsiella pneumoniae, rabies, crimenia-Congo hemorrhagic fever virus, human herpesvirus type 4, pseudomonas aeruginosa, human Bokavirus type 2, leishmania donovani, yersinia pestis, staphylococcus epidermidis, vibrio cholerae, chlamydia pneumoniae, adenovirus type 4, dengue virus, streptococcus suis, novel coronavirus, vibrio fluvialis, legionella pneumophila, laxafever virus, burkholderia glabra, adenovirus type 31, Clostridium tetani, escherichia coli, stenotrophomonas maltophilia, monkey pox virus, mumps virus type 4, rhinovirus type a, mucor global, human bocavirus type 3, franciscensis, streptococcus pneumoniae, toxoplasma gondii, staphylococcus aureus, staphylococcus cephalosum, mumps virus, staphylococcus saprophyticus, aeromonas hydrophila, treponema pallidum, mycobacterium leprae, brucella abortus, human bocavirus type 1, bordetella parapertussis, rhinovirus type C, rift valley fever virus, ebola virus, campylobacter jejuni, human bocavirus type 4, adenovirus type 2, human metapneumovirus, burkholderia pseudomelitensis, Pertussis, burkholderia, influenza A virus, burkholderia melitensis, ehrlichia glabra, vibrio parahaemolyticus, type 2 herpes simplex virus, type 1 herpes simplex virus, enterovirus D, chikungunya virus, human coronavirus NL63, guangzhou pipe round nematode, plasmodium vivax, neisseria gonorrhoeae, norovirus GI, borrelia burgdorferi, bursaphenous nematode, aspergillus fumigatus, lumbricus-like nematode, aspergillus niger, clonorchis sinensis, cochinella americana, yersinia pneumoconica, candida tropicalis, cochinella duodenalis, endomonas histolytica, enterovirus B, leptospira question mark, echinococcus multi-chamber, emmerce pig encephalitis, epidemic type B virus, at least two of enterobiasis/enterobiasis, neisseria meningitidis, enterovirus A, wuzehnia (Ban Shi filarial), haemophilus influenzae, fever with thrombocytopenia syndrome virus, adenovirus D17; when the pathogen comprises rabies virus, the combination of nucleic acid molecules comprises a 1-1) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 63, and/or a 1-2) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 211; where the pathogen comprises rhinovirus type A, the combination of nucleic acid molecules comprises a 2-1) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 88, and/or a 2-2) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 236; When the pathogen comprises Staphylococcus cephali, the combination of nucleic acid molecules comprises a 3-1) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 95, and/or a 3-2) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 243; where the pathogen comprises Botrytis pertussis, the combination of nucleic acid molecules comprises a 4-1) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 112, and/or a 4-2) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 260; When the pathogen comprises enterovirus D, the combination of nucleic acid molecules comprises a 5-1) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 119 and/or a 5-2) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 267; when the pathogen comprises Cronobacter sakazakii, the combination of nucleic acid molecules comprises a 6-1) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 1, and/or a 6-2) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 149; Where the pathogen comprises Candida albicans, the combination of nucleic acid molecules comprises a 7-1) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 2, and/or a 7-2) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 150; When the pathogen comprises Enterobacter cloacae, the combination of nucleic acid molecules comprises a 8-1) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 3, and/or a 8-2) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 151; Where the pathogen comprises Candida glabrata, the combination of nucleic acid molecules comprises a 9-1) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 4, and/or a 9-2) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 152; where the pathogen comprises influenza C virus, the combination of nucleic acid molecules comprises a 10-1) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 5, and/or a 10-2) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 153; Where the pathogen comprises Staphylococcus haemolyticus, the combination of nucleic acid molecules comprises a 11-1) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 6, and/or a 11-2) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 154; Where the pathogen comprises human respiratory virus type 3, the combination of nucleic acid molecules comprises a 12-1) a nucleic acid molecule comprising the nucleotide sequence set forth in SEQ ID NO. 7, and/or a 12-2) a nucleic acid molecule comprising the nucleotide sequence set forth in SEQ ID NO. 155; Where the pathogen comprises norovirus GII, the combination of nucleic acid molecules comprises a 13-1) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 8, and/or a 13-2) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 156; Where the pathogen comprises severe acute respiratory syndrome associated coronavirus, the combination of nucleic acid molecules comprises a 14-1) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 9, and/or a 14-2) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 157; when the pathogen comprises Acinetobacter baumannii, the combination of nucleic acid molecules comprises a 15-1) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 10, and/or a 15-2) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 158; where the pathogen comprises enterococcus faecalis, the combination of nucleic acid molecules comprises a 16-1) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 11, and/or a 16-2) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 159; Where the pathogen comprises adenovirus type 21, the combination of nucleic acid molecules comprises a 17-1) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 12, and/or a 17-2) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 160; where the pathogen comprises a Hancheng virus, the combination of nucleic acid molecules comprises a 18-1) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 13 and/or a 18-2) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 161; Where the pathogen comprises human immunodeficiency virus type 1, the combination of nucleic acid molecules comprises a 19-1) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 14, and/or a 19-2) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 162; where the pathogen comprises measles virus, the combination of nucleic acid molecules comprises a 20-1) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 15 and/or a 20-2) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 163; Where the pathogen comprises Streptococcus pyogenes, the combination of nucleic acid molecules comprises a 21-1) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 16, and/or a 21-2) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 164; When the pathogen comprises Vibrio vulnificus, the combination of nucleic acid molecules comprises a 22-1) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 17, and/or a 22-2) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 165; Where the pathogen comprises Corynebacterium diphtheriae, the combination of nucleic acid molecules comprises a 23-1) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 18, and/or a 23-2) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 166; Where the pathogen comprises parvovirus B19, the combination of nucleic acid molecules comprises a 24-1) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 19 and/or a 24-2) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 167; Where the pathogen comprises Campylobacter fetus, the combination of nucleic acid molecules comprises a 25-1) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 20, and/or a 25-2) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 168; Where the pathogen comprises enterococcus faecium, the combination of nucleic acid molecules comprises a 26-1) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 21, and/or a 26-2) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 169; Where the pathogen comprises Streptococcus agalactiae, the combination of nucleic acid molecules comprises a 27-1) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 22, and/or a 27-2) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 170; where the pathogen comprises respiratory syncytial virus, the combination of nucleic acid molecules comprises a 28-1) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 23, and/or a 28-2) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 171; where the pathogen comprises Bacillus anthracis, the combination of nucleic acid molecules comprises a 29-1) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 24, and/or a 29-2) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 172; Where the pathogen comprises Clostridium perfringens, the combination of nucleic acid molecules comprises a 30-1) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 25, and/or a 30-2) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 173; where the pathogen comprises Mycoplasma pneumoniae, the combination of nucleic acid molecules comprises a 31-1) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 26, and/or a 31-2) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 174; Where the pathogen comprises Yersinia enterocolitica, the combination of nucleic acid molecules comprises a 32-1) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 27, and/or a 32-2) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 175; where the pathogen comprises a rubella virus, the combination of nucleic acid molecules comprises a 33-1) a nucleic acid molecule comprising the nucleotide sequence set forth in SEQ ID NO. 28, and/or a 33-2) a nucleic acid molecule comprising the nucleotide sequence set forth in SEQ ID NO. 176; Where the pathogen comprises a middle east respiratory syndrome coronavirus, the combination of nucleic acid molecules comprises a 34-1) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 29, and/or a 34-2) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 177; where the pathogen comprises poliovirus type 1, the combination of nucleic acid molecules comprises a 35-1) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 30, and/or a 35-2) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 178; where the pathogen comprises human mumps virus type 2, the combination of nucleic acid molecules comprises a 36-1) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 31 and/or a 36-2) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 179; Where the pathogen comprises coronavirus HCoV-OC43, the combination of nucleic acid molecules comprises a 37-1) nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 32, and/or a 37-2) nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 180; where the pathogen comprises Cryptosporidium human, the combination of nucleic acid molecules comprises a 38-1) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 33, and/or a 38-2) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 181; When the pathogen comprises Prinsepia utilis, luo Waci g (Prinsepia) Rickettsia, the combination of nucleic acid molecules comprises a 39-1) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 34, and/or a 39-2) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 182; where the pathogen comprises Cryptococcus gartertiaryana, the combination of nucleic acid molecules comprises a 40-1) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 35, and/or a 40-2) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 183; Where the pathogen comprises Shigella dysenteriae, the combination of nucleic acid molecules comprises a 41-1) nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 36, and/or a 41-2) nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 184; where the pathogen comprises Staphylococcus Wobbe, the combination of nucleic acid molecules comprises a 42-1) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 37, and/or a 42-2) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 185; Where the pathogen comprises rhinovirus type B, the combination of nucleic acid molecules comprises a 43-1) nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 38, and/or a 43-2) nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 186; where the pathogen comprises a Zika virus, the combination of nucleic acid molecules comprises a 44-1) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO:39, and/or a 44-2) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO: 187; where the pathogen comprises human coronavirus HKU1, the combination of nucleic acid molecules comprises a 45-1) a nucleic acid molecule comprising the nucleotide sequence set forth in SEQ ID NO. 40, and/or a 45-2) a nucleic acid molecule comprising the nucleotide sequence set forth in SEQ ID NO. 188; Where the pathogen comprises nocardia astrotricha, the combination of nucleic acid molecules comprises a 46-1) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 41, and/or a 46-2) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 189; where the pathogen comprises enterovirus C, the combination of nucleic acid molecules comprises a 47-1) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 42, and/or a 47-2) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 190; When the pathogen comprises Aspergillus flavus, the combination of nucleic acid molecules comprises a 48-1) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 43, and/or a 48-2) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 191; where the pathogen comprises human herpesvirus type 3, the combination of nucleic acid molecules comprises a 49-1) nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 44, and/or a 49-2) nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 192; Where the pathogen comprises human herpesvirus type 5, the combination of nucleic acid molecules comprises a 50-1) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 45, and/or a 50-2) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 193; Where the pathogen comprises a West Nile virus, the combination of nucleic acid molecules comprises a 51-1) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 46, and/or a 51-2) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 194; Where the pathogen comprises Salmonella enterica, the combination of nucleic acid molecules comprises a 52-1) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 47, and/or a 52-2) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 195; Where the pathogen comprises influenza B virus, the combination of nucleic acid molecules comprises a 53-1) nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 48 and/or a 53-2) nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 196; When the pathogen comprises Schistosoma japonicum, the combination of nucleic acid molecules comprises a 54-1) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 49 and/or a 54-2) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 197; when the pathogen comprises rotavirus group A, the combination of nucleic acid molecules comprises a 55-1) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 50, and/or a 55-2) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 198; where the pathogen comprises Clostridium difficile, the combination of nucleic acid molecules comprises a 56-1) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 51, and/or a 56-2) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 199; When the pathogen comprises Mycobacterium tuberculosis, the combination of nucleic acid molecules comprises a 57-1) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 52, and/or a 57-2) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 200; Where the pathogen comprises Yersinia pseudotuberculosis, the combination of nucleic acid molecules comprises a 58-1) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 53, and/or a 58-2) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 201; Where the pathogen comprises a phagocyte anaplasma, the combination of nucleic acid molecules comprises a 59-1) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 54, and/or a 59-2) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 202; Where the pathogen comprises Chlamydia psittaci, the combination of nucleic acid molecules comprises a 60-1) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 55 and/or a 60-2) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 203; Where the pathogen comprises Listeria monocytogenes, the combination of nucleic acid molecules comprises a 61-1) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO:56, and/or a 61-2) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO: 204; Where the pathogen comprises Cryptococcus neoformans, the combination of nucleic acid molecules comprises a 62-1) a nucleic acid molecule comprising the nucleotide sequence set forth in SEQ ID NO. 57, and/or a 62-2) a nucleic acid molecule comprising the nucleotide sequence set forth in SEQ ID NO. 205; When the pathogen comprises A.terreus, the combination of nucleic acid molecules comprises a 63-1) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 58, and/or a 63-2) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 206; Where the pathogen comprises Acinetobacter calcoaceticus, the combination of nucleic acid molecules comprises a 64-1) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 59, and/or a 64-2) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 207; where the pathogen comprises human respiratory virus type 1, the combination of nucleic acid molecules comprises a 65-1) a nucleic acid molecule comprising the nucleotide sequence set forth in SEQ ID NO. 60, and/or a 65-2) a nucleic acid molecule comprising the nucleotide sequence set forth in SEQ ID NO. 208; Where the pathogen comprises rickettsia, the combination of nucleic acid molecules comprises a 66-1) a nucleic acid molecule comprising the nucleotide sequence set forth in SEQ ID NO. 61, and/or a 66-2) a nucleic acid molecule comprising the nucleotide sequence set forth in SEQ ID NO. 209; Where the pathogen comprises Klebsiella pneumoniae, the combination of nucleic acid molecules comprises a 67-1) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 62, and/or a 67-2) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 210; Where the pathogen comprises a Crimedes Congo hemorrhagic fever virus, the combination of nucleic acid molecules comprises a 68-1) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 64 and/or a 68-2) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 212; Where the pathogen comprises human herpesvirus type 4, the combination of nucleic acid molecules comprises a 69-1) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 65, and/or a 69-2) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 213; when the pathogen comprises Pseudomonas aeruginosa, the combination of nucleic acid molecules comprises a 70-1) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 66, and/or a 70-2) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 214; Where the pathogen comprises human bocavirus type 2, the combination of nucleic acid molecules comprises a 71-1) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID No. 67, and/or a 71-2) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID No. 215; Where the pathogen comprises Leishmania donovani, the combination of nucleic acid molecules comprises a 72-1) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 68, and/or a 72-2) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 216; When the pathogen comprises Yersinia pestis, the combination of nucleic acid molecules comprises a 73-1) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO:69, and/or a 73-2) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO: 217; Where the pathogen comprises Staphylococcus epidermidis, the combination of nucleic acid molecules comprises a 74-1) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 70, and/or a 74-2) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 218; When the pathogen comprises Vibrio cholerae, the combination of nucleic acid molecules comprises a 75-1) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 71, and/or a 75-2) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 219; Where the pathogen comprises Chlamydia pneumoniae, the combination of nucleic acid molecules comprises a 76-1) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO:72, and/or a 76-2) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO: 220; Where the pathogen comprises adenovirus type 4, the combination of nucleic acid molecules comprises a 77-1) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 73, and/or a 77-2) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 221; Where the pathogen comprises dengue virus, the combination of nucleic acid molecules comprises a 78-1) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 74, and/or a 78-2) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 222; Where the pathogen comprises Streptococcus suis, the combination of nucleic acid molecules comprises a 79-1) a nucleic acid molecule comprising the nucleotide sequence set forth in SEQ ID NO. 75, and/or a 79-2) a nucleic acid molecule comprising the nucleotide sequence set forth in SEQ ID NO. 223; Where the pathogen comprises a novel coronavirus, the combination of nucleic acid molecules comprises a 80-1) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 76, and/or a 80-2) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 224; When the pathogen comprises Vibrio fluvialis, the combination of nucleic acid molecules comprises a 81-1) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 77, and/or a 81-2) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 225; When the pathogen comprises Staphylococcus lugdunensis, the combination of nucleic acid molecules comprises a 82-1) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO:78, and/or a 82-2) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO: 226; Where the pathogen comprises Legionella pneumophila, the combination of nucleic acid molecules comprises a 83-1) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 79, and/or a 83-2) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 227; Where the pathogen comprises a lassa fever virus, the combination of nucleic acid molecules comprises a 84-1) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 80, and/or a 84-2) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 228; Where the pathogen comprises Burkholderia gladioli, the combination of nucleic acid molecules comprises a 85-1) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 81, and/or a 85-2) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 229; where the pathogen comprises adenovirus type 31, the combination of nucleic acid molecules comprises a 86-1) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 82, and/or a 86-2) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 230; Where the pathogen comprises Clostridium tetani, the combination of nucleic acid molecules comprises a 87-1) nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 83, and/or a 87-2) nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 231; When the pathogen comprises Escherichia coli, the combination of nucleic acid molecules comprises a 88-1) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 84, and/or a 88-2) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 232; When the pathogen comprises stenotrophomonas maltophilia, the combination of nucleic acid molecules comprises a 89-1) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 85, and/or a 89-2) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 233; Where the pathogen comprises a monkey poxvirus, the combination of nucleic acid molecules comprises a 90-1) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 86 and/or a 90-2) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 234; Where the pathogen comprises human mumps virus type 4, the combination of nucleic acid molecules comprises a 91-1) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 87, and/or a 91-2) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 235; where the pathogen comprises Mucor racemosus, the combination of nucleic acid molecules comprises a 92-1) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 89, and/or a 92-2) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 237; Where the pathogen comprises human bocavirus type 3, the combination of nucleic acid molecules comprises a 93-1) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID No. 90, and/or a 93-2) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID No. 238; where the pathogen comprises Francisella tularensis, the combination of nucleic acid molecules comprises a 94-1) a nucleic acid molecule comprising the nucleotide sequence set forth in SEQ ID NO. 91, and/or a 94-2) a nucleic acid molecule comprising the nucleotide sequence set forth in SEQ ID NO. 239; Where the pathogen comprises Streptococcus pneumoniae, the combination of nucleic acid molecules comprises a 95-1) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 92, and/or a 95-2) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 240; Where the pathogen comprises toxoplasma gondii, the combination of nucleic acid molecules comprises a 96-1) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 93, and/or a 96-2) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 241; Where the pathogen comprises Staphylococcus aureus, the combination of nucleic acid molecules comprises a 97-1) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 94, and/or a 97-2) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 242; Where the pathogen comprises mumps virus, the combination of nucleic acid molecules comprises a 98-1) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 96 and/or a 98-2) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 244; Where the pathogen comprises Staphylococcus saprophyticus, the combination of nucleic acid molecules comprises a 99-1) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 97, and/or a 99-2) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 245; When the pathogen comprises Aeromonas hydrophila, the combination of nucleic acid molecules comprises a 100-1) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 98, and/or a 100-2) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 246; Where the pathogen comprises treponema pallidum, the combination of nucleic acid molecules comprises a 101-1) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID No. 99, and/or a 101-2) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID No. 247; Where the pathogen comprises Mycobacterium leprae, the combination of nucleic acid molecules comprises a 102-1) a nucleic acid molecule comprising the nucleotide sequence set forth in SEQ ID NO. 100, and/or a 102-2) a nucleic acid molecule comprising the nucleotide sequence set forth in SEQ ID NO. 248; where the pathogen comprises Brucella abortus, the combination of nucleic acid molecules comprises a 103-1) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 101 and/or a 103-2) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 249; Where the pathogen comprises human bocavirus type 1, the combination of nucleic acid molecules comprises a 104-1) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID No. 102, and/or a 104-2) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID No. 250; where the pathogen comprises bordetella parapertussis, the combination of nucleic acid molecules comprises a 105-1) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 103, and/or a 105-2) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 251; Where the pathogen comprises rhinovirus type C, the combination of nucleic acid molecules comprises a 106-1) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 104, and/or a 106-2) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 252; where the pathogen comprises a rift valley fever virus, the combination of nucleic acid molecules comprises a 107-1) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 105 and/or a 107-2) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 253; where the pathogen comprises an Ebola virus, the combination of nucleic acid molecules comprises a 108-1) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 106, and/or a 108-2) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 254; When the pathogen comprises Campylobacter jejuni, the combination of nucleic acid molecules comprises a 109-1) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 107, and/or a 109-2) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 255; where the pathogen comprises human bocavirus type 4, the combination of nucleic acid molecules comprises a 110-1) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID No. 108, and/or a 110-2) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID No. 256; where the pathogen comprises adenovirus type 2, the combination of nucleic acid molecules comprises a 111-1) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 109, and/or a 111-2) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 257; where the pathogen comprises a human metapneumovirus, the combination of nucleic acid molecules comprises a 112-1) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 110, and/or a 112-2) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 258; when the pathogen comprises Burkholderia pseudomeliotis, the combination of nucleic acid molecules comprises a 113-1) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO:111, and/or a 113-2) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO: 259; where the pathogen comprises influenza A virus, the combination of nucleic acid molecules comprises a 114-1) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 113, and/or a 114-2) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 261; where the pathogen comprises Burkholderia meliotidis, the combination of nucleic acid molecules comprises a 115-1) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 114, and/or a 115-2) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 262; Where the pathogen comprises E.chaffeensis, the combination of nucleic acid molecules comprises a 116-1) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 115 and/or a 116-2) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 263; when the pathogen comprises Vibrio parahaemolyticus, the combination of nucleic acid molecules comprises a 117-1) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 116, and/or a 117-2) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 264; where the pathogen comprises herpes simplex virus type 2, the combination of nucleic acid molecules comprises a 118-1) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 117, and/or a 118-2) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 265; Where the pathogen comprises herpes simplex virus type 1, the combination of nucleic acid molecules comprises a 119-1) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 118, and/or a 119-2) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 266; Where the pathogen comprises chikungunya virus, the combination of nucleic acid molecules comprises a 120-1) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 120, and/or a 120-2) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 268; Where the pathogen comprises human coronavirus NL63, the combination of nucleic acid molecules comprises a 121-1) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 121 and/or a 121-2) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 269; where the pathogen comprises a Guangdong nematode, the combination of nucleic acid molecules comprises a 122-1) a nucleic acid molecule comprising the nucleotide sequence set forth in SEQ ID NO. 122, and/or a 122-2) a nucleic acid molecule comprising the nucleotide sequence set forth in SEQ ID NO. 270; where the pathogen comprises plasmodium vivax, the combination of nucleic acid molecules comprises a 123-1) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID No. 123, and/or a 123-2) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID No. 271; Where the pathogen comprises Neisseria gonorrhoeae, the combination of nucleic acid molecules comprises a 124-1) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 124, and/or a 124-2) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 272; Where the pathogen comprises norovirus GI, the combination of nucleic acid molecules comprises a 125-1) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 125, and/or a 125-2) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 273; where the pathogen comprises borrelia burgdorferi, the combination of nucleic acid molecules comprises a 126-1) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 126, and/or a 126-2) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 274; Where the pathogen comprises a Bursaphelenchus, the combination of nucleic acid molecules comprises a 127-1) a nucleic acid molecule comprising the nucleotide sequence set forth in SEQ ID NO:127, and/or a 127-2) a nucleic acid molecule comprising the nucleotide sequence set forth in SEQ ID NO: 275; Where the pathogen comprises A.fumigatus, the combination of nucleic acid molecules comprises a 128-1) a nucleic acid molecule comprising the nucleotide sequence set forth in SEQ ID NO. 128, and/or a 128-2) a nucleic acid molecule comprising the nucleotide sequence set forth in SEQ ID NO. 276; Where the pathogen comprises a lumbricus-like nematode, the combination of nucleic acid molecules comprises a 129-1) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 129, and/or a 129-2) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 277; Where the pathogen comprises Aspergillus niger, the combination of nucleic acid molecules comprises a 130-1) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 130, and/or a 130-2) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 278; When the pathogen comprises clonorchis sinensis, the combination of nucleic acid molecules comprises a 131-1) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 131, and/or a 131-2) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 279; Where the pathogen comprises ancylostoma americanum, the combination of nucleic acid molecules comprises a 132-1) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 132, and/or a 132-2) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 280; When the pathogen comprises P.yersicolor, the combination of nucleic acid molecules comprises a 133-1) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO:133, and/or a 133-2) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO: 281; when the pathogen comprises candida tropicalis, the combination of nucleic acid molecules comprises a 134-1) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 134 and/or a 134-2) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 282; Where the pathogen comprises a species of E.duodenum, the combination of nucleic acid molecules comprises a 135-1) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO:135, and/or a 135-2) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO: 283; Where the pathogen comprises Entamoeba histolytica, the combination of nucleic acid molecules comprises a 136-1) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 136, and/or a 136-2) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 284; when the pathogen comprises enterovirus B, the combination of nucleic acid molecules comprises a 137-1) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 137, and/or a 137-2) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 285; where the pathogen comprises leptospira interrogans, the combination of nucleic acid molecules comprises a 138-1) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 138, and/or a 138-2) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 286; where the pathogen comprises Echinococcus multilocularis, the combination of nucleic acid molecules comprises a 139-1) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 139 and/or a 139-2) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 287; where the pathogen comprises taenia solium, the combination of nucleic acid molecules comprises a 140-1) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 140, and/or a 140-2) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 288; Where the pathogen comprises an epidemic encephalitis B virus, the combination of nucleic acid molecules comprises a 141-1) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 141, and/or a 141-2) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO. 289; where the pathogen comprises vermicular-shaped enterobiasis/enterobiasis, the nucleic acid molecule combination comprises a 142-1) a nucleic acid molecule comprising the nucleotide sequence set forth in SEQ ID NO:142, and/or a 142-2) a nucleic acid molecule comprising the nucleotide sequence set forth in SEQ ID NO: 290; Where the pathogen comprises Neisseria meningitidis, the combination of nucleic acid molecules comprises a 143-1) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO:143, and/or a 143-2) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO: 291; when the pathogen comprises enterovirus A, the combination of nucleic acid molecules comprises a 144-1) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 144 and/or a 144-2) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 292; Where the pathogen comprises Wuzella (Ban Shi filarial), the combination of nucleic acid molecules comprises a 145-1) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO:145, and/or a 145-2) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO: 293; Where the pathogen comprises haemophilus influenzae, the combination of nucleic acid molecules comprises a 146-1) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 146, and/or a 146-2) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 294; Where the pathogen comprises a febrile thrombocytopenia syndrome virus, the combination of nucleic acid molecules comprises a 147-1) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO:147 and/or a 147-2) a nucleic acid molecule comprising the nucleotide sequence shown in SEQ ID NO: 295; where the pathogen comprises adenovirus D17, the combination of nucleic acid molecules comprises a 148-1) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 148, and/or a 148-2) a nucleic acid molecule comprising the nucleotide sequence shown as SEQ ID NO. 296.
  2. 2. The combination of nucleic acid molecules of a pathogen standard according to claim 1, The nucleic acid molecule is single-stranded DNA; Preferably, the nucleic acid molecule is double-stranded DNA; Preferably, the nucleic acid molecule is double-stranded DNA and one or more nucleotides in the nucleic acid molecule are biotin or a derivative modified nucleotide thereof.
  3. 3. A blocked nucleic acid molecule combination obtained by blocking a nucleic acid molecule combination of a pathogen standard according to any one of claims 1-2.
  4. 4. The blocked nucleic acid molecule combination of claim 3, Sealing by adopting a form of protein binding and/or packaging; Preferably, the nucleic acid molecule is single-stranded DNA, blocked in a protein-bound form; preferably, the nucleic acid molecule is double-stranded DNA, and is blocked in a packaged form.
  5. 5. The blocked nucleic acid molecule combination of any one of claims 3 to 4, The nucleic acid molecule is single-stranded DNA, and is blocked by mixing the nucleic acid molecule combination of the pathogen standard according to any one of claims 1-2, single-stranded binding protein and hemoglobin, and reacting to obtain the pathogen standard; preferably, the nucleic acid molecule is double-stranded DNA, and the blocking is performed by mixing and reacting the nucleic acid molecule combination, avidin or derivatives thereof of the pathogen standard according to any one of claims 1-2, wherein one or more nucleotides in the nucleic acid molecule are modified nucleotides of biotin or derivatives thereof.
  6. 6. A pathogen standard comprising the combination of nucleic acid molecules of the pathogen standard of any one of claims 1-2 or the blocked combination of nucleic acid molecules of any one of claims 3-5.
  7. 7. The pathogen standard according to claim 6, wherein, The pathogen standard comprising the blocked nucleic acid molecule combination of any one of claims 3-5; Preferably, the pathogen standard further comprises a matrix component; Preferably, the matrix composition comprises human nucleic acid, glycerol, EDTA, phenol red, and water.
  8. 8. The pathogen standard according to claim 7, wherein, The nucleic acid molecule is double-stranded DNA, and the matrix component further comprises peptidoglycan and chitin.
  9. 9. Use of the nucleic acid molecule combination of the pathogen standard of any one of claims 1-2, the blocked nucleic acid molecule combination of any one of claims 3-5 or the pathogen standard of any one of claims 6-8 in any one of b 1) -b 2): b1 Detecting a pathogen; b2 A product for detecting pathogens is prepared.
  10. 10. A product comprising the nucleic acid molecule combination of the pathogen standard of any one of claims 1-2, the blocked nucleic acid molecule combination of any one of claims 3-5, or the pathogen standard of any one of claims 6-8; preferably, the product comprises a pathogen standard according to any one of claims 6-8; Preferably, the product is a reagent, kit or kit of reagents.

Description

Nucleic acid molecule combination of pathogen standard and application thereof Technical Field The invention belongs to the technical field of sequencing, and particularly relates to a nucleic acid molecule combination of pathogen standard substances and application thereof. Background Recently, metagenomic second-generation high-throughput sequencing technology (mNGS) and pathogen microorganism-targeted sequencing (tNGS) have begun to be widely used in many research and clinical fields, which utilize sequencing and alignment of nucleic acids to identify the vast majority of microorganism species in a sample. During the application, each of mNGS and tNGS is tested using a negative control (NTC) and a positive control (PTC) to monitor the presence of anomalies during the test to ensure the reliability and stability of the test results. Typically, NTCs will use a pathogen-free nucleic acid of human genome, the test results of which can be used to monitor the reagent background and environmental background during the course of this run of experiments, to distinguish responsible from background pathogens in the sample batch. PTC typically incorporates a known pathogen or pathogen nucleic acid into a human genomic nucleic acid that is free of the pathogen nucleic acid, and the test results can be used to monitor whether abnormalities occur during this batch of experiments, resulting in undetectable positive pathogens. The pathogen in the PTC may be in the form of inactivated bacteria to mimic the actual infection of a bacterial fluid, or fragmented genomic nucleic acid to mimic the status of pathogen free nucleic acid (e.g., cfDNA) in a sterile fluid. Currently, commonly used PTC generally employ one or more known clinically pathogenic pathogens, such as acinetobacter baumannii, staphylococcus aureus. Part of the positive standard substance also has probiotics as PTC, so that pathogens in the positive standard substance are prevented from polluting the sample to be tested, and the interpretation is prevented from being correctly reported. Therefore, the number of pathogens in the PTC used at present cannot be too large, one is to avoid cross contamination between samples in the same batch, and the other is to avoid laboratory environmental pollution caused by long-term use of PTC in the laboratory, which results in sample contamination between laboratory batches. However, the performance of the mNGS and tNGS products of different companies is currently uneven, and the detection level of the target pathogen of the products cannot be completely monitored by only using one or more known clinical pathogenic pathogens, especially for monitoring the detection actual conditions in the pathogen spectrum of tNGS products. But can easily cause contamination problems for increasing the variety of PTC pathogens. In addition, for a complete PTC, its function requires monitoring the entire process of mNGS and tNGS, including extraction, pooling, hybridization and sequencing. The extraction and monitoring of the bacteria liquid is also a difficult problem, because different pathogens are cultured in different modes, the preparation of different pathogens and the simultaneous incorporation of PTC (Positive temperature coefficient) preparation are time-consuming and labor-consuming, and some pathogens are often aggregated together or have volume differences, so that the quantity of the incorporated pathogens cannot be controlled accurately. Therefore, PTC of a variety of pathogens can be prepared without contamination problems, easy to operate and low cost, and the difficulty of accurately controlling the number of copies of pathogen standard is great. Disclosure of Invention It is an object of a first aspect of the present invention to provide a combination of nucleic acid molecules of a pathogen standard. It is an object of the second aspect of the present invention to provide a blocked nucleic acid molecule combination. It is an object of a third aspect of the present invention to provide pathogen standard. The object of the fourth aspect of the present invention is to provide a combination of nucleic acid molecules of the pathogen standard of the first aspect of the present invention, a combination of blocked nucleic acid molecules of the second aspect or the use of the pathogen standard of the third aspect. The object of the fifth aspect of the invention is to provide a product. In order to achieve the above purpose, the technical scheme adopted by the invention is as follows: In a first aspect of the invention, there is provided a combination of nucleic acid molecules of a pathogen standard, The pathogen comprises at least two (such as two, three, four or five; preferably five) of rabies virus, rhinovirus A type, staphylococcus cephalus, pertussis baud bacteria, enterovirus D, or The pathogen includes Cronobacter sakazakii, candida albicans, enterobacter cloacae, candida glabrata, influenza C virus, staphylococcus haemolyt